SNAP-25 Blocking Peptide (#BLP-NR001) is the original antigen used for immunization during Anti-SNAP-25 Antibody (#ANR-001) generation. The blocking peptide binds and ‘blocks’ Anti-SNAP-25 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of rat brain membranes:1. Anti-SNAP-25 Antibody (#ANR-001), (1:1000).
2. Anti-SNAP-25 Antibody, preincubated with SNAP-25 Blocking Peptide (BLP-NR001).
- Rat brain sections.
- GST fusion protein with the sequence MAEDADMRNELEEMQRRADQLADESLESTRRMLQLVEESKDAGIRTLVMLDEQGEQLERIEEGMDQINKDMKEAEKNLTDLGKFCGLCVCPCNKLKSSDAYKKAWGNNQDGVVASQPARV VDEREQMAISGGFIRRVTNDARENEMDENLEQVSGIIGNLRHMALDMGNEIDTQNRQIDRIMEKADSNKTRIDEANQRATKMLGSG, corresponding to the full-length of rat SNAP-25 (Accession P60881).