Cat #: BLP-NR001
Type: GST fusion protein
Form: Lyophilized powder
SNAP-25 Blocking Peptide (#BLP-NR001) is the original antigen used for immunization during Anti-SNAP-25 Antibody (#ANR-001) generation. The blocking peptide binds and ‘blocks’ Anti-SNAP-25 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
WB-western blot, IHC-Immunohistochemistry
For research purposes only, not for human use
Demonstration of Pre-adsorption control
- Western blot analysis of rat brain membranes:1. Anti-SNAP-25 Antibody (#ANR-001), (1:1000).
2. Anti-SNAP-25 Antibody, preincubated with SNAP-25 Blocking Peptide (BLP-NR001).
- Rat brain sections.
- GST fusion protein with the sequence MAEDADMRNELEEMQRRADQLADESLESTRRMLQLVEESKDAGIRTLVMLDEQGEQLERIEEGMDQINKDMKEAEKNLTDLGKFCGLCVCPCNKLKSSDAYKKAWGNNQDGVVASQPARV VDEREQMAISGGFIRRVTNDARENEMDENLEQVSGIIGNLRHMALDMGNEIDTQNRQIDRIMEKADSNKTRIDEANQRATKMLGSG, corresponding to the full-length of rat SNAP-25 (Accession P60881).
Accession (Uniprot) Number P60881
Peptide Confirmation Confirmed by DNA sequence and SDSPAGE.
Purity >95% (SDS-PAGE)
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 μl PBS.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 5 µg fusion protein per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.