Trichostatin A

Antibiotic A300, TSA
A Powerful Histone Deacetylase Inhibitor
    Cat #: T-300
    Alternative Name Antibiotic A300, TSA
  • Lyophilized Powder
  • Bioassay Tested
  • Origin Streptomyces platensis.
    Source Natural
    MW: 302.4
    Purity: >98%
    Effective concentration 100 nM - 1 µM.
    Structure
    • Trichostatin A
    Chemical name (2E,4E,6R)-7-(4-(Dimethylamino)phenyl)-N-hydroxy-4,6- di methyl-7-oxo-2,4-heptadienamide.
    Molecular formula C17H22N2O3.
    CAS No.: 58880-19-6.
    Activity Trichostatin A, an antifungal antibiotic, irreversibly inhibits histone deacetylase1. It could also promote apoptosis2 and in some cases even inhibits the proliferation of some cancer cells3.
    References-Activity
    1. Yoshida, M. et al. (1990) J. Biol. Chem265, 17174.
    2. Yamashita, Yet al. (2003) Int. J. Cancer 103, 572.
    3. Donadelli, Met al. (2003) Mol. Carcinog38, 59.
    Shipping and storage Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
    Solubility DMSO or ethanol. Centrifuge all product preparations before use (10000 x g 5 min).
    Storage of solutions Up to two weeks at 4°C or six months at -20°C.
    Our bioassay
    • Trichostatin A
      Alomone Labs Trichostatin A inhibits histone deacetylases in NIH/3T3 cells.
      Cells from logarithmic were incubated overnight with different concentrations of Trichostatin A (#T-300). Cell proteins were resolved by SDS-PAGE and probed with anti-acetylated histone antibodies.
    References - Scientific background
    1. Tsuji, N. et al. (1976) J. Antibiot. (Tokyo)29, 1.
    2. Yoshida, M. et al. (1990) J. Biol. Chem265, 17174.
    3. Yoshida, M. et al. (1995) Bioessays 17, 423.
    4. Hoshikawa, Y. et al. (1994) Exp. Cell Res214, 189.
    5. Futamura, M. et al. (1995) Oncogene 10, 1119.
    6. Papeleu, P. et al. (2003) J. Hepatol39, 374.
    7. Yamashita, Y. et al. (2003) Int. J. Cancer 103, 572.
    8. Donadelli, M. et al. (2003) Mol. Carcinog38, 59.
    9. Ailenberg, M. et al. (2002) Biochem. Biophys. Res. Commun298, 110.
    10. Sawa, H. et al. (2002) Brain Tumor Pathol19, 77.
    11. Shaker, S. et al. (2003) Leuk. Res27, 437.
    Scientific background

    Trichostatin A (TSA), an antifungal antibiotic derived from Streptomyces, is a potent and reversible inhibitor of histone deacetylase.1 TSA causes accumulation of highly acetylated histone molecules in mammalian cells,2 that leads to chromatin relaxation and modulation of gene transcription/expression.3

    TSA blocked cell cycle progression at the G1 phase in HeLa cells.4 In NIH 3T3 cells, it induced reversion of oncogenic ras-transformed cells to a normal morphology.5 TSA did not induce apoptosis in primary rat hepatocytes,6 however, it induced cell-cycle arrest and apoptosis in human hepatoma cell lines (HepG2, Huh-7).7

    Unlike sodium butyrate, Trichostatin A action is very specific and reversible and thus, became a widely used tool to study the consequences of histone acetylation in vitro and in vivo.

    TSA strongly inhibited the cellular growth of nine pancreatic adenocarcinoma cell lines. It induced up-regulation of p21 (WAF1/CIP1), cell cycle arrest at G2, and apoptosis. Therefore, TSA may have a potential utility in treatment of pancreatic cancer.8

    Recently, TSA was reported as a potent inhibitor of Gelatinase A, a matrix metalloproteinase implicated in metastasis,9 and it also inhibited the secretion of vascular endothelial growth factor (VEGF) from human glioblastoma cells.10 Preclinical evaluation of TSA in combination with DNA methylation inhibitors, demonstrated a synergistic anti-neoplastic effect against myeloid leukemic cells.11

    Last update: 24/01/2020

    Trichostatin A (#T-300) is a highly pure, natural, and biologically active compound.

    For research purposes only, not for human use

    Applications

    Specifications

    Scientific Background

    Citations

    Citations
    Product citations
    1. Ling, Let al. (2013) J. Mol. Endocrinol. 50, 279.
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