Overview
Type: Synthetic peptide
Form: Lyophilized powder
TRPV4 Blocking Peptide (#BLP-CC034) is the original antigen used for immunization during Anti-TRPV4 Antibody (#ACC-034) generation. The blocking peptide binds and ‘blocks’ Anti-TRPV4 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only, not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain lysates:1. Anti-TRPV4 Antibody (#ACC-034), (1:200).
2. Anti-TRPV4 Antibody, preincubated with TRPV4 Blocking Peptide (#BLP-CC034).
Western blot analysis of ND7/23 Mouse neuroblastoma /rat dorsal root ganglion neuron hybrid cell line lysate:1. Anti-TRPV4 Antibody (#ACC-034), (1:200).
2. Anti-TRPV4 Antibody, preincubated with TRPV4 Blocking Peptide (#BLP-CC034).
Expression of TRPV4 in rat DRGImmunohistochemical staining of rat dorsal root ganglion (DRG) frozen sections using Anti-TRPV4 Antibody (#ACC-034). A. TRPV4 (red) in DRG neurons. B. Staining with mouse anti-Parvalbumin (green) in the same DRG section. C. Confocal merge of TRPV4 and Parvalbumin demonstrates colocalization.
Western blot analysis of rat brain membranes:1. Guinea pig Anti-TRPV4 Antibody (#Acc-034-GP), (1:1500).
2. Guinea pig Anti-TRPV4 Antibody, preincubated with TRPV4 Blocking Peptide (#BLP-CC034).
Western blot analysis of rat DRG lysates:3. Guinea pig Anti-TRPV4 Antibody (#Acc-034-GP), (1:200).
4. Guinea pig Anti-TRPV4 Antibody, preincubated with TRPV4 Blocking Peptide (#BLP-CC034).
Properties
Sequence
- CDGHQQGYAPKWRAEDAPL, corresponding to amino acid residues 853-871 of rat TRPV4 (Accession Q9ERZ8).
Accession (Uniprot) Number Q9ERZ8
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
