Overview
Type: Synthetic peptide
Form: Lyophilized powder
TRPV4 Blocking Peptide (#BLP-CC034) is the original antigen used for immunization during Anti-TRPV4 Antibody (#ACC-034) generation. The blocking peptide binds and ‘blocks’ Anti-TRPV4 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only, not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain lysates:1. Anti-TRPV4 Antibody (#ACC-034), (1:200).
2. Anti-TRPV4 Antibody, preincubated with TRPV4 Blocking Peptide (#BLP-CC034).
Western blot analysis of ND7/23 Mouse neuroblastoma /rat dorsal root ganglion neuron hybrid cell line lysate:1. Anti-TRPV4 Antibody (#ACC-034), (1:200).
2. Anti-TRPV4 Antibody, preincubated with TRPV4 Blocking Peptide (#BLP-CC034).
Expression of TRPV4 in rat DRGImmunohistochemical staining of rat dorsal root ganglion (DRG) frozen sections using Anti-TRPV4 Antibody (#ACC-034). A. TRPV4 (red) in DRG neurons. B. Staining with mouse anti-Parvalbumin (green) in the same DRG section. C. Confocal merge of TRPV4 and Parvalbumin demonstrates colocalization.
Western blot analysis of rat brain membranes:1. Guinea pig Anti-TRPV4 Antibody (#Acc-034-GP), (1:1500).
2. Guinea pig Anti-TRPV4 Antibody, preincubated with TRPV4 Blocking Peptide (#BLP-CC034).
Western blot analysis of rat DRG lysates:3. Guinea pig Anti-TRPV4 Antibody (#Acc-034-GP), (1:200).
4. Guinea pig Anti-TRPV4 Antibody, preincubated with TRPV4 Blocking Peptide (#BLP-CC034).
Properties
Sequence
- CDGHQQGYAPKWRAEDAPL, corresponding to amino acid residues 853-871 of rat TRPV4 (Accession Q9ERZ8).
Accession (Uniprot) Number Q9ERZ8
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
Shipping and Ordering information
Need Help With This Product?
Our reagent specialists are here to help you find the best product for your application. Please call or email us and we will be happy to help you find the right product for the job.
Call us at
+972 2587 2202 or Email Us
