Overview
Type: Synthetic peptide
Form: Lyophilized powder
A Blocking peptide for Anti-TRPV4 Antibody (#ACC-034), Anti-TRPV4-ATTO Fluor-550 Antibody (#ACC-034-AO) and Guinea pig Anti-TRPV4 Antibody (#AGP-147). Intended to be used as a negative control.
Applications: bl
For research purposes only, not for human use
Applications
Applications
Blocking
Western blot analysis of rat brain lysates:1. Anti-TRPV4 Antibody (#ACC-034), (1:200).
2. Anti-TRPV4 Antibody, preincubated with TRPV4 Blocking Peptide (#BLP-CC034).
Western blot analysis of ND7/23 Mouse neuroblastoma /rat dorsal root ganglion neuron hybrid cell line lysate:1. Anti-TRPV4 Antibody (#ACC-034), (1:200).
2. Anti-TRPV4 Antibody, preincubated with TRPV4 Blocking Peptide (#BLP-CC034).
Expression of TRPV4 in rat DRGImmunohistochemical staining of rat dorsal root ganglion (DRG) frozen sections using Anti-TRPV4 Antibody (#ACC-034). A. TRPV4 (red) in DRG neurons. B. Staining with mouse anti-Parvalbumin (green) in the same DRG section. C. Confocal merge of TRPV4 and Parvalbumin demonstrates colocalization.
Expression of TRPV4 in rat DRGImmunohistochemical staining of rat dorsal root ganglia (DRG) frozen sections with Anti-TRPV4-ATTO Fluor-550 Antibody (#ACC-034-AO), (1:20). TRPV4 is present in DRG neurons (red). Note that staining is stronger in small cells (arrows). Hoechst 33342 is used as the counterstain.
Western blot analysis of rat brain membranes:1. Guinea pig Anti-TRPV4 Antibody (#AGP-147), (1:1500).
2. Guinea pig Anti-TRPV4 Antibody, preincubated with TRPV4 Blocking Peptide (#BLP-CC034).
Western blot analysis of rat DRG lysates:3. Guinea pig Anti-TRPV4 Antibody (#AGP-147), (1:200).
4. Guinea pig Anti-TRPV4 Antibody, preincubated with TRPV4 Blocking Peptide (#BLP-CC034).
Properties
Properties
Sequence
- CDGHQQGYAPKWRAEDAPL, corresponding to amino acid residues 853-871 of rat TRPV4 (Accession Q9ERZ8).
Accession (Uniprot) Number Q9ERZ8
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
Shipping and Ordering information
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