Overview
Type: Synthetic peptide
Form: Lyophilized Powder
GPR132 (extracellular) Blocking Peptide (#BLP-GR036) is the original antigen used for immunization during Anti-GPR132 (extracellular) Antibody (#AGR-036) generation. The blocking peptide binds and ‘blocks’ Anti-GPR132 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat dorsal root ganglia lysate:1. Anti-GPR132 (extracellular) Antibody (#AGR-036), (1:200).
2. Anti-GPR132 (extracellular) Antibody, preincubated with GPR132 (extracellular) Blocking Peptide (BLP-GR036).
Western blot analysis of mouse brain membranes (lanes 1 and 3) and rat brain membranes (lanes 2 and 4):1-2. Anti-GPR132 (extracellular) Antibody (#AGR-036), (1:200).
3-4. Anti-GPR132 (extracellular) Antibody, preincubated with GPR132 (extracellular) Blocking Peptide (BLP-GR036).
Western blot analysis of mouse BV-2 microglia cell line lysate:1. Anti-GPR132 (extracellular) Antibody (#AGR-036), (1:200).
2. Anti-GPR132 (extracellular) Antibody, preincubated with GPR132 (extracellular) Blocking Peptide (BLP-GR036).
Properties
Sequence
- Peptide CFEPLRMNSKIAGYH, corresponding to amino acid residues 184 - 198 of mouse GPR132 (Accession Q9Z282).
Accession (Uniprot) Number Q9Z282
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
