Overview
Type: Synthetic peptide
Form: Lyophilized Powder
GFRAL (extracellular) Blocking Peptide (#BLP-NT029) is the original antigen used for immunization during Anti-GFRAL (extracellular) Antibody (#ANT-029) generation. The blocking peptide binds and ‘blocks’ Anti-GFRAL (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain lysates (lanes 1 and 4), mouse brain lysates (lanes 2 and 5) and rat brain stem lysates (lanes 3 and 6):1-3. Anti-GFRAL (extracellular) Antibody (#ANT-029), (1:400).
4-6. Anti-GFRAL (extracellular) Antibody, preincubated with GFRAL (extracellular) Blocking Peptide (BLP-NT029).
Expression of GFRAL in rat nucleus of the solitary tract.Immunohistochemical staining of perfusion-fixed frozen rat brain sections using Anti-GFRAL (extracellular) Antibody (#ANT-029), (1:200), followed by goat anti-rabbit-AlexaFluor-488. A. GFRAL immunoreactivity (green) appears in cells lining the wall of the 4th ventricle (down pointing arrow) and in neurons in the nucleus of the solitary tract (horizontal arrows). B. Pre-incubation of the antibody with GFRAL (extracellular) Blocking Peptide (BLP-NT029), suppressed staining. Cell nuclei are stained with DAPI (blue).
Properties
Sequence
- Peptide (C)YPTPNVKDISSYERK, corresponding to amino acid residues 320 - 334 of rat GFRAL (Accession D3ZB94).
Accession (Uniprot) Number D3ZB94
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
