Extracellular antibodies are ideal research tools for cell-surface proteins.
No permeabilization/fixation because using extracellular antibodies allows you to work with live cells!
Benefits of live cell imaging:
✓ Monitor cell movement
✓ Study protein transport across the cell
✓ Investigate protein internalization
N-type Ca2+ channels are mainly involved in neurotransmitter release from central neurons, including glutamate, γ-aminobutyric acid (GABA), acetylcholine, dopamine and noradrenaline neurons.