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  • The expected molecular weight (MW) listed in the product datasheet is based solely on the size of the target protein’s amino acid sequence. It’s important to remember that many factors can affect the banding pattern of your western blot including: (1) the existence of a splice variant, (2) the quality of the loaded sample, (3) the protein extraction method, and (4) the protein transfer conditions. To achieve accurate results, you may need to systematically adjust the protein extraction method or the protein transfer conditions.

    • If the band’s MW is below the expected MW it could be due to a splice variant with a slightly different MW.
      • Heat the samples at 70°C for 10 min.
      • Increase the transfer time.
    • If the band’s MW is above the expected MW, it could be due to post-translational modifications.
    • In either event, you should use a blocking peptide as a negative control.

  • Some toxins and peptides are soluble in DMSO; you can find this information in the product specification section. For these products, prepare a concentrated stock solution first.

    1. Centrifuge vial (10,000 x g, 5 minutes) before adding solvent.
    2. Dissolve the lyophilized reagent in DMSO. We recommend keeping a concentrated stock solution at 1-10 mM (100-1000X higher than the final working concentration).
    3. Once the peptide is completely dissolved in DMSO, slowly dilute the peptide in pure water (or buffer) to the desired final working concentration.

    Note: We recommend that you maintain a DMSO concentration as low as possible. For cell assays, a final concentration of 0.1–0.5% DMSO (v/v) is considered acceptable. For other experiments, 5% DMSO (v/v) is recommended; adjust this according to your experimental requirements.

  • FAQ 3FAQ 3FAQ 3FAQ 3

Category: Conjugated Venom-Derived Toxins
Category: GABA (B) Receptors
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36 Results
  • γ-Aminobutyric acid
    Bioassay Tested
    γ-Aminobutyric acid
    A Ligand of GABA(A), GABA(B) Receptors and GABA Transporters
    • Product Code: G-110
    • CAS No.: 56-12-2
    • MW: 103.1
  • Osu1 Toxin-ATTO Fluor-647N
    Bioassay Tested
    Osu1 Toxin-ATTO Fluor-647N
    A fluorescent probe for imaging and sensitive detection of KV1.5 channels.
    • Product Code: STK-010-FRN
    • Short Description: A fluorescent probe for imaging and sensitive detection of KV1.5 channels.
    • Applications: Electrophysiology, Direct flow cytometry, Fluorescence staining, Live cell imaging, Immunofluorescence
    • MW: ~8418 Da
  • α-Dendrotoxin-ATTO Fluor-647N
    Bioassay Tested
    α-Dendrotoxin-ATTO Fluor-647N
    A fluorescent probe for imaging and sensitive detection of Kv1.1, Kv1.2, and Kv1.6 channels.
    • Product Code: D-350-FRN
    • Applications: Electrophysiology, Direct flow cytometry, Fluorescence staining, Live cell imaging, Immunofluorescence
    • MW: ~7990 Da
  • Iberiotoxin-ATTO Fluor-647N
    Bioassay Tested
    Iberiotoxin-ATTO Fluor-647N
    A fluorescent probe for imaging and sensitive detection of KCa1.1 (BK) K+ channels
    • Product Code: STI-400-FRN
    • Short Description: A fluorescent probe for imaging and sensitive detection of KCa1.1 (BK) K+ channels
    • Applications: Electrophysiology, Direct flow cytometry, Fluorescence staining, Live cell imaging, Immunofluorescence
    • MW: 4858 Da
  • Hm1a-ATTO Fluor 647N
    Bioassay Tested
    Hm1a-ATTO Fluor 647N
    A fluorescent probe for imaging and sensitive detection of NaV1.1 voltage-gated sodium channels
    • Product Code: STH-600-FRN
    • Short Description: A fluorescent probe for imaging and sensitive detection of NaV1.1 voltage-gated sodium channels
    • Applications: Electrophysiology, Direct flow cytometry, Fluorescence staining, Live cell imaging, Immunofluorescence
    • MW: 4625 Da
  • GsMTx-4-ATTO Fluor-647N
    Bioassay Tested
    GsMTx-4-ATTO Fluor-647N
    A fluorescent probe for imaging and sensitive detection of mechanosensitive ion channels (Piezo1, TRPC1, TRPC6) and NaV1.7 sodium channels
    • Product Code: STG-100-FRN
    • Applications: Electrophysiology, Direct flow cytometry, Fluorescence staining, Live cell imaging, Immunofluorescence
    • MW: 4723 Da
  • ATX-II-ATTO Fluor-647N
    Bioassay Tested
    ATX-II-ATTO Fluor-647N
    A fluorescent probe for imaging and sensitive detection of NaV sodium channels
    • Product Code: STA-700-FRN
    • Short Description: A fluorescent probe for imaging and sensitive detection of NaV sodium channels
    • Applications: Electrophysiology, Direct flow cytometry, Fluorescence staining, Live cell imaging, Immunofluorescence
    • MW: 5561 Da
  • ω-Conotoxin MVIIC-ATTO Fluor 647N
    Bioassay Tested
    ω-Conotoxin MVIIC-ATTO Fluor 647N
    A fluorescent probe for imaging and sensitive detection of N-type (CaV2.2) and P/Q-type (CaV2.1) voltage-gated calcium channels in neurons
    • Product Code: C-150-FRN
    • Short Description: A fluorescent probe for imaging and sensitive detection of N-type (CaV2.2) and P/Q-type (CaV2.1) voltage-gated calcium channels in neurons
    • Applications: Electrophysiology, Direct flow cytometry, Fluorescence staining, Live cell imaging, Immunofluorescence
    • MW: 3376 Da
  • ω-Agatoxin IVA-ATTO Fluor-647N
    Bioassay Tested
    ω-Agatoxin IVA-ATTO Fluor-647N
    A fluorescent probe for imaging and sensitive detection of P/Q-type (CaV2.1) calcium channels
    • Product Code: STA-500-FRN
    • Short Description: A fluorescent probe for imaging and sensitive detection of P/Q-type (CaV2.1) calcium channels
    • Applications: Electrophysiology, Direct flow cytometry, Fluorescence staining, Live cell imaging, Immunofluorescence
    • MW: ~6144 Da
  • α-Bungarotoxin-ATTO Fluor-647N
    Bioassay Tested
    α-Bungarotoxin-ATTO Fluor-647N
    A fluorescent probe for imaging and sensitive detection of neuromuscular junctions, GABA(A) receptors, nAChRs, and α-Bungarotoxin binding sites
    • Product Code: B-100-FRN
    • Applications: Electrophysiology, Direct flow cytometry, Fluorescence staining, Live cell imaging, Immunofluorescence
    • MW: ~8925 Da
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