Microglia Markers

Microglia Markers

Microglia are the resident immune cells of the central nervous system (CNS). They originate from yolk sac progenitors during embryonic development and serve as the brain’s primary innate immune defense. In the healthy CNS, microglia continuously survey the microenvironment, contributing to synaptic pruning, neurogenesis, and tissue homeostasis. Upon injury, infection, or neurodegeneration, they become activated and undergo morphological and functional changes, including cytokine release, phagocytosis, and antigen presentation.

To support microglia research, Alomone Labs offers a wide range of high-quality rabbit and guinea pig polyclonal antibodies, including both conjugated and unconjugated formats. These antibodies are validated for use in multiple applications such as immunohistochemistry (IHC), immunofluorescence (IF), Western blotting (WB), and flow cytometry (FACS), enabling precise detection and characterization of microglial markers across experimental systems.

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FAQs

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  • The expected molecular weight (MW) listed in the product datasheet is based solely on the size of the target protein’s amino acid sequence. It’s important to remember that many factors can affect the banding pattern of your western blot including: (1) the existence of a splice variant, (2) the quality of the loaded sample, (3) the protein extraction method, and (4) the protein transfer conditions. To achieve accurate results, you may need to systematically adjust the protein extraction method or the protein transfer conditions.

    • If the band’s MW is below the expected MW it could be due to a splice variant with a slightly different MW.
      • Heat the samples at 70°C for 10 min.
      • Increase the transfer time.
    • If the band’s MW is above the expected MW, it could be due to post-translational modifications.
    • In either event, you should use a blocking peptide as a negative control.

  • Some toxins and peptides are soluble in DMSO; you can find this information in the product specification section. For these products, prepare a concentrated stock solution first.

    1. Centrifuge vial (10,000 x g, 5 minutes) before adding solvent.
    2. Dissolve the lyophilized reagent in DMSO. We recommend keeping a concentrated stock solution at 1-10 mM (100-1000X higher than the final working concentration).
    3. Once the peptide is completely dissolved in DMSO, slowly dilute the peptide in pure water (or buffer) to the desired final working concentration.

    Note: We recommend that you maintain a DMSO concentration as low as possible. For cell assays, a final concentration of 0.1–0.5% DMSO (v/v) is considered acceptable. For other experiments, 5% DMSO (v/v) is recommended; adjust this according to your experimental requirements.

  • FAQ 3FAQ 3FAQ 3FAQ 3

90 Results
  • Anti-Adenosine A<sub>2A</sub> Receptor (extracellular) Antibody
    Anti-Adenosine A2A Receptor (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Adenosine A2A Receptor
    • Product Code: AAR-008
    • Short Description: A Rabbit Polyclonal Antibody to Adenosine A2A Receptor
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunohistochemistry
    • Target: ADORA2A, A2AAR, Adenosine A2aR
  • Anti-Adenosine A2A Receptor (extracellular)-FITC Antibody
    Anti-Adenosine A2A Receptor (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to Adenosine A2A Receptor Conjugated to the Fluorescent Dye FITC
    • Product Code: AAR-008-F
    • Short Description: A Rabbit Polyclonal Antibody to Adenosine A2A Receptor Conjugated to the Fluorescent Dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: ADORA2A, A2AAR, Adenosine A2aR
  • Anti-Adenosine A<sub>2A</sub> Receptor (extracellular)-PE Antibody
    Anti-Adenosine A2A Receptor (extracellular)-PE Antibody
    A Rabbit Polyclonal Antibody to Adenosine A2A Receptor (extracellular) conjugated to the fluorescent dye R-Phycoerythrin (R-PE)
    • Product Code: AAR-008-PE
    • Short Description: A Rabbit Polyclonal Antibody to Adenosine A2A Receptor (extracellular) conjugated to the fluorescent dye R-Phycoerythrin (R-PE)
    • Host: Rabbit
    • Label: R-PE
    • Reactivity: Rat, Mouse
    • Applications: Flow cytometry
    • Target: ADORA2A, A2AAR, Adenosine A2aR
  • Anti-Adenosine A<sub>2A</sub> Receptor Antibody
    KO Validate'
    Anti-Adenosine A2A Receptor Antibody
    A Rabbit Polyclonal Antibody to Adenosine A2A Receptor
    • Product Code: AAR-002
    • Short Description: A Rabbit Polyclonal Antibody to Adenosine A2A Receptor
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: ADORA2A, A2AAR, Adenosine A2aR
  • Anti-AXL (extracellular) Antibody
    Anti-AXL (extracellular) Antibody
    A Rabbit Polyclonal antibody to AXL (extracellular)
    • Product Code: ATR-032
    • Short Description: A Rabbit Polyclonal antibody to AXL (extracellular)
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunohistochemistry
    • Target: AXL Receptor Tyrosine Kinase, Tyrosine-Protein Kinase Receptor UFO, AXL Oncogene, Tyro7, UFO
  • Anti-AXL (extracellular)-FITC Antibody
    Anti-AXL (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to AXL (extracellular) conjugated to the fluorescent dye FITC
    • Product Code: ATR-032-F
    • Short Description: A Rabbit Polyclonal Antibody to AXL (extracellular) conjugated to the fluorescent dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: AXL Receptor Tyrosine Kinase, Tyrosine-Protein Kinase Receptor UFO, AXL Oncogene, Tyro7, UFO, ARK
  • Anti-C3aR1 (extracellular) Antibody
    Anti-C3aR1 (extracellular) Antibody
    A Rabbit Polyclonal antibody to C3aR1 (extracellular)
    • Product Code: AAR-036
    • Short Description: A Rabbit Polyclonal antibody to C3aR1 (extracellular)
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunohistochemistry
    • Target: Complement C3a Receptor 1, C3AR, C3a Anaphylatoxin Chemotactic Receptor
  • Anti-C3aR1 (extracellular)-FITC Antibody
    Anti-C3aR1 (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to C3aR1 (extracellular) conjugated to the fluorescent dye FITC
    • Product Code: AAR-036-F
    • Short Description: A Rabbit Polyclonal Antibody to C3aR1 (extracellular) conjugated to the fluorescent dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: Complement C3a Receptor 1, C3AR, C3a Anaphylatoxin Chemotactic Receptor
  • Anti-C3aR1 (extracellular)-mFluor™ Violet 450 Antibody
    Anti-C3aR1 (extracellular)-mFluor™ Violet 450 Antibody
    A Rabbit Polyclonal Antibody to C3aR1 (extracellular) conjugated to the fluorescent dye mFluor™ Violet 450 fluorophore.
    • Product Code: AAR-036-V
    • Short Description: A Rabbit Polyclonal Antibody to C3aR1 (extracellular) conjugated to the fluorescent dye mFluor™ Violet 450 fluorophore.
    • Host: Rabbit
    • Label: mFluor™ Violet 450
    • Reactivity: Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: Complement C3a Receptor 1, C3AR, C3a Anaphylatoxin Chemotactic Receptor
  • Anti-CD39 (extracellular) Antibody
    Anti-CD39 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to CD39
    • Product Code: ANT-065
    • Short Description: A Rabbit Polyclonal Antibody to CD39
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot
    • Target: Ectonucleoside triphosphate diphosphohydrolase 1, NTPDase 1, Ecto-ATP diphosphohydrolase 1, Ecto-ATPDase 1, Ecto-apyrase, Lymphoid cell activation antigen, ENTPD1
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