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The expected molecular weight (MW) listed in the product datasheet is based solely on the size of the target protein’s amino acid sequence. It’s important to remember that many factors can affect the banding pattern of your western blot including: (1) the existence of a splice variant, (2) the quality of the loaded sample, (3) the protein extraction method, and (4) the protein transfer conditions. To achieve accurate results, you may need to systematically adjust the protein extraction method or the protein transfer conditions.
- If the band’s MW is below the expected MW it could be due to a splice variant with a slightly different MW.
  • Heat the samples at 70°C for 10 min.
  • Increase the transfer time.
- If the band’s MW is above the expected MW, it could be due to post-translational modifications.
- In either event, you should use a blocking peptide as a negative control.
Some toxins and peptides are soluble in DMSO; you can find this information in the product specification section. For these products, prepare a concentrated stock solution first.
1. Centrifuge vial (10,000 x g, 5 minutes) before adding solvent.
2. Dissolve the lyophilized reagent in DMSO. We recommend keeping a concentrated stock solution at 1-10 mM (100-1000X higher than the final working concentration).
3. Once the peptide is completely dissolved in DMSO, slowly dilute the peptide in pure water (or buffer) to the desired final working concentration.
Note: We recommend that you maintain a DMSO concentration as low as possible. For cell assays, a final concentration of 0.1–0.5% DMSO (v/v) is considered acceptable. For other experiments, 5% DMSO (v/v) is recommended; adjust this according to your experimental requirements.
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Cystic Fibrosis Transmembrane Conductance Regulator (CFTR)

Cannabinoid Receptors

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Trimethylapigenin

A Blocker of KV1.5 and KV11.1 and an Activator of CFTR Channels
- Product Code: T-145
- Short Description: A Blocker of KV1.5 and KV11.1 and an Activator of CFTR Channels
- CAS No.: 5631-70-9
- MW: 312.3
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Olvanil

An Activator of TRPV1 Channel and Cannabinoid Receptors
- Product Code: O-115
- Short Description: An Activator of TRPV1 Channel and Cannabinoid Receptors
- CAS No.: 58493-49-5
- MW: 417.6
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Cannabichromene

An Activator of TRPA1 Channels and an Antagonist of Cannabinoid Receptor 1 and TRPM8 Channels
- Product Code: C-315
- Short Description: An Activator of TRPA1 Channels and an Antagonist of Cannabinoid Receptor 1 and TRPM8 Channels
- CAS No.: 20675-51-8
- MW: 314.5
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Arvanil

An Agonist of TRPV1 Channels and a Ligand of CB1 Receptors
- Product Code: A-335
- Short Description: An Agonist of TRPV1 Channels and a Ligand of CB1 Receptors
- CAS No.: 128007-31-8
- MW: 439.64

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