Cardiac Failure

Cardiac Failure

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  • The expected molecular weight (MW) listed in the product datasheet is based solely on the size of the target protein’s amino acid sequence. It’s important to remember that many factors can affect the banding pattern of your western blot including: (1) the existence of a splice variant, (2) the quality of the loaded sample, (3) the protein extraction method, and (4) the protein transfer conditions. To achieve accurate results, you may need to systematically adjust the protein extraction method or the protein transfer conditions.

    • If the band’s MW is below the expected MW it could be due to a splice variant with a slightly different MW.
      • Heat the samples at 70°C for 10 min.
      • Increase the transfer time.
    • If the band’s MW is above the expected MW, it could be due to post-translational modifications.
    • In either event, you should use a blocking peptide as a negative control.

  • Some toxins and peptides are soluble in DMSO; you can find this information in the product specification section. For these products, prepare a concentrated stock solution first.

    1. Centrifuge vial (10,000 x g, 5 minutes) before adding solvent.
    2. Dissolve the lyophilized reagent in DMSO. We recommend keeping a concentrated stock solution at 1-10 mM (100-1000X higher than the final working concentration).
    3. Once the peptide is completely dissolved in DMSO, slowly dilute the peptide in pure water (or buffer) to the desired final working concentration.

    Note: We recommend that you maintain a DMSO concentration as low as possible. For cell assays, a final concentration of 0.1–0.5% DMSO (v/v) is considered acceptable. For other experiments, 5% DMSO (v/v) is recommended; adjust this according to your experimental requirements.

  • FAQ 3FAQ 3FAQ 3FAQ 3

286 Results
  • Anti-β1-Adrenergic Receptor (extracellular)-FITC Antibody
    KO Validate'
    Anti-β1-Adrenergic Receptor (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to β1-Adrenergic Receptor  (extracellular) conjugated to the fluorescent dye FITC
    • Product Code: AAR-023-F
    • Short Description: A Rabbit Polyclonal Antibody to β1-Adrenergic Receptor  (extracellular) conjugated to the fluorescent dye FITC
    • Host: Rabbit
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
  • Apamin
    Bioassay Tested
    Apamin
    A blocker of small conductance Ca2+-Activated K+ Channels (SK Type, KCa2)
    • Product Code: STA-200
    • Applications: Electrophysiology
    • CAS No.: 24345-16-2
    • MW: 2027.3 Da
  • Apamin-Biotin
    Bioassay Tested
    Apamin-Biotin
    A biotinylated apamin for imaging and sensitive detection of KCa2 potassium channels (SK channels)
    • Product Code: STA-200-B
    • Short Description: A biotinylated apamin for imaging and sensitive detection of KCa2 potassium channels (SK channels)
    • Applications: Electrophysiology, Immunofluorescence
    • MW: 2367 Da
  • Aquaporin 2 Blocking Peptide
    Aquaporin 2 Blocking Peptide
    A Blocking Peptide for Anti-Aquaporin 2 Antibody
    • Product Code: BLP-QP002
    • Short Description: A Blocking Peptide for Anti-Aquaporin 2 Antibody
    • Applications: Western blot, Immunohistochemistry
    • Target: AQP2
  • ATPA
    Bioassay Tested
    ATPA
    A Potent and Selective Agonist of GluK1 Kainate Receptor
    • Product Code: A-480
    • Short Description: A Potent and Selective Agonist of GluK1 Kainate Receptor
    • CAS No.: 140158-50-5
    • MW: 228.25
  • Atrasentan
    Bioassay Tested
    Atrasentan
    A Potent and Selective Antagonist of Endothelin A Receptors
    • Product Code: A-455
    • Short Description: A Potent and Selective Antagonist of Endothelin A Receptors
    • CAS No.: 173937-91-2
    • MW: 510.62
  • ATX-II
    Bioassay Tested
    ATX-II
    A potent activator of voltage-gated sodium NaV channels that primarily targets cardiac NaV1.5 channels by delaying their fast inactivation
    • Product Code: STA-700
    • Short Description: A potent activator of voltage-gated sodium NaV channels that primarily targets cardiac NaV1.5 channels by delaying their fast inactivation
    • Applications: Electrophysiology
    • CAS No.: 60748-45-0
    • MW: 4935 Da
  • Avosentan
    Bioassay Tested
    Avosentan
    A Selective Antagonist of Endothelin A Receptors
    • Product Code: A-355
    • Short Description: A Selective Antagonist of Endothelin A Receptors
    • CAS No.: 290815-26-8
    • MW: 479.51
  • Azemiopsin
    Bioassay Tested
    Azemiopsin
    An antagonist of muscle α1/β1/γ/δ nicotinic acetylcholine receptors (nAChR)
    • Product Code: STA-100
    • Short Description: An antagonist of muscle α1/β1/γ/δ nicotinic acetylcholine receptors (nAChR)
    • Applications: Electrophysiology
    • CAS No.: 1391936-86-9
    • MW: 2541 Da
  • BDS-I
    Bioassay Tested
    BDS-I
    A blocker of KV potassium channels and a modulator that slows the inactivation of NaV sodium channels (particularly NaV1.7)
    • Product Code: STB-400
    • Short Description: A blocker of KV potassium channels and a modulator that slows the inactivation of NaV sodium channels (particularly NaV1.7)
    • Applications: Electrophysiology
    • CAS No.: 207621-38-3
    • MW: 4708 Da
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