Cardiac Failure

Cardiac Failure

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  • The expected molecular weight (MW) listed in the product datasheet is based solely on the size of the target protein’s amino acid sequence. It’s important to remember that many factors can affect the banding pattern of your western blot including: (1) the existence of a splice variant, (2) the quality of the loaded sample, (3) the protein extraction method, and (4) the protein transfer conditions. To achieve accurate results, you may need to systematically adjust the protein extraction method or the protein transfer conditions.

    • If the band’s MW is below the expected MW it could be due to a splice variant with a slightly different MW.
      • Heat the samples at 70°C for 10 min.
      • Increase the transfer time.
    • If the band’s MW is above the expected MW, it could be due to post-translational modifications.
    • In either event, you should use a blocking peptide as a negative control.

  • Some toxins and peptides are soluble in DMSO; you can find this information in the product specification section. For these products, prepare a concentrated stock solution first.

    1. Centrifuge vial (10,000 x g, 5 minutes) before adding solvent.
    2. Dissolve the lyophilized reagent in DMSO. We recommend keeping a concentrated stock solution at 1-10 mM (100-1000X higher than the final working concentration).
    3. Once the peptide is completely dissolved in DMSO, slowly dilute the peptide in pure water (or buffer) to the desired final working concentration.

    Note: We recommend that you maintain a DMSO concentration as low as possible. For cell assays, a final concentration of 0.1–0.5% DMSO (v/v) is considered acceptable. For other experiments, 5% DMSO (v/v) is recommended; adjust this according to your experimental requirements.

  • FAQ 3FAQ 3FAQ 3FAQ 3

286 Results
  • Guinea pig Anti-KCNN2 (K<sub>Ca</sub>2.2, SK2) Antibody
    Guinea pig Anti-KCNN2 (KCa2.2, SK2) Antibody
    A Guinea Pig Polyclonal Antibody to KCNN2 (KCa2.2, SK2) Channel
    • Product Code: APC-028-GP
    • Short Description: A Guinea Pig Polyclonal Antibody to KCNN2 (KCa2.2, SK2) Channel
    • Host: Guinea Pig
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Western blot
    • Target: SKCa2, Small conductance calcium-activated potassium channel protein 2, Apamin-sensitive small conductance Ca2+-dependent K+ channel
  • Guinea pig Anti-K<sub>ir</sub>2.1/KCNJ2 Antibody
    Guinea pig Anti-Kir2.1/KCNJ2 Antibody
    A Guinea Pig Polyclonal Antibody to Kir2.1 (KCNJ2) Channel
    • Product Code: APC-026-GP
    • Short Description: A Guinea Pig Polyclonal Antibody to Kir2.1 (KCNJ2) Channel
    • Host: Guinea Pig
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot
    • Target: Inward rectifier potassium channel 2, IRK1, HIRK1, LQT7, SQT3
  • Guinea pig Anti-K<sub>V</sub>4.2 Antibody
    Guinea pig Anti-KV4.2 Antibody
    A Guinea Pig Polyclonal Antibody to KV4.2 Channel
    • Product Code: APC-023-GP
    • Short Description: A Guinea Pig Polyclonal Antibody to KV4.2 Channel
    • Host: Guinea Pig
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: Voltage-gated potassium channel subfamily D member 2, KCND2, Shal1, RK5
  • Guinea pig Anti-Na<sub>V</sub>1.5 (SCN5A) Antibody
    Guinea pig Anti-NaV1.5 (SCN5A) Antibody
    A Guinea Pig Polyclonal Antibody to NaV1.5 (SCN5A) (493-511) Channel
    • Product Code: ASC-005-GP
    • Short Description: A Guinea Pig Polyclonal Antibody to NaV1.5 (SCN5A) (493-511) Channel
    • Host: Guinea Pig
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: Sodium channel protein type 5 subunit alpha, SKM2
  • Guinea pig Anti-Na<sub>V</sub>1.8 (SCN10A) Antibody
    Guinea pig Anti-NaV1.8 (SCN10A) Antibody
    A Guinea Pig Polyclonal Antibody to NaV1.8 (SCN10A) Channel
    • Product Code: ASC-016-GP
    • Short Description: A Guinea Pig Polyclonal Antibody to NaV1.8 (SCN10A) Channel
    • Host: Guinea Pig
    • Label: Unconjugated
    • Reactivity: Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: PN3, SNS, Sodium channel protein type 10 subunit alpha
  • Guinea pig Anti-TRPC3 Antibody
    Guinea pig Anti-TRPC3 Antibody
    A Guinea Pig Polyclonal Antibody to TRPC3 Channel
    • Product Code: ACC-016-GP
    • Short Description: A Guinea Pig Polyclonal Antibody to TRPC3 Channel
    • Host: Guinea Pig
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Western blot, Immunohistochemistry
    • Target: Short transient receptor potential channel 3, Transient receptor protein 3, TRP3
  • Guinea pig Anti-TRPC6 Antibody
    Guinea pig Anti-TRPC6 Antibody
    A Guinea Pig Polyclonal Antibody to TRPC6 Channel
    • Product Code: ACC-017-GP
    • Short Description: A Guinea Pig Polyclonal Antibody to TRPC6 Channel
    • Host: Guinea Pig
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: Short transient receptor potential channel 6, Transient receptor protein 6, TRP6
  • HA-1077
    Bioassay Tested
    HA-1077
    A Rho/Rok Inhibitor
    • Product Code: H-220
    • Short Description: A Rho/Rok Inhibitor
    • CAS No.: 103745-39-7
    • MW: 364.3
  • HCN2 Blocking Peptide
    HCN2 Blocking Peptide
    A Blocking Peptide for Anti-HCN2 Antibody
    • Product Code: BLP-PC030
    • Short Description: A Blocking Peptide for Anti-HCN2 Antibody
    • Applications: Western blot, Immunohistochemistry
    • Target: HCN2
  • HCN4 Blocking Peptide
    HCN4 Blocking Peptide
    A Blocking Peptide for Anti-HCN4 Antibody
    • Product Code: BLP-PC052
    • Short Description: A Blocking Peptide for Anti-HCN4 Antibody
    • Applications: Western blot, Immunohistochemistry
    • Target: HCN4
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