Cardiac Failure

Cardiac Failure

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  • The expected molecular weight (MW) listed in the product datasheet is based solely on the size of the target protein’s amino acid sequence. It’s important to remember that many factors can affect the banding pattern of your western blot including: (1) the existence of a splice variant, (2) the quality of the loaded sample, (3) the protein extraction method, and (4) the protein transfer conditions. To achieve accurate results, you may need to systematically adjust the protein extraction method or the protein transfer conditions.

    • If the band’s MW is below the expected MW it could be due to a splice variant with a slightly different MW.
      • Heat the samples at 70°C for 10 min.
      • Increase the transfer time.
    • If the band’s MW is above the expected MW, it could be due to post-translational modifications.
    • In either event, you should use a blocking peptide as a negative control.

  • Some toxins and peptides are soluble in DMSO; you can find this information in the product specification section. For these products, prepare a concentrated stock solution first.

    1. Centrifuge vial (10,000 x g, 5 minutes) before adding solvent.
    2. Dissolve the lyophilized reagent in DMSO. We recommend keeping a concentrated stock solution at 1-10 mM (100-1000X higher than the final working concentration).
    3. Once the peptide is completely dissolved in DMSO, slowly dilute the peptide in pure water (or buffer) to the desired final working concentration.

    Note: We recommend that you maintain a DMSO concentration as low as possible. For cell assays, a final concentration of 0.1–0.5% DMSO (v/v) is considered acceptable. For other experiments, 5% DMSO (v/v) is recommended; adjust this according to your experimental requirements.

  • FAQ 3FAQ 3FAQ 3FAQ 3

286 Results
  • Anti-Ca<sub>V</sub>1.2a (CACNA1C) Antibody
    Anti-CaV1.2a (CACNA1C) Antibody
    A Rabbit Polyclonal Antibody to CaV1.2a (CACNA1C) Channel
    • Product Code: ACC-013
    • Short Description: A Rabbit Polyclonal Antibody to CaV1.2a (CACNA1C) Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry
    • Target: Cardiac type α1C, Voltage-dependent L-type calcium channel subunit alpha-1C.
  • Anti-Ca<sub>V</sub>pan α1 Antibody
    Anti-CaVpan α1 Antibody
    A Rabbit Pan Polyclonal Antibody to CaV1 and CaV2 Subunits
    • Product Code: ACC-004
    • Short Description: A Rabbit Pan Polyclonal Antibody to CaV1 and CaV2 Subunits
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunoprecipitation, Immunohistochemistry
    • Target: Voltage-dependent L-type calcium channel subunit alpha-1C, Cacna1c
  • Anti-CHERP Antibody
    Anti-CHERP Antibody
    A Rabbit Polyclonal Antibody to CHERP
    • Product Code: ACC-330
    • Short Description: A Rabbit Polyclonal Antibody to CHERP
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: Calcium homeostasis endoplasmic reticulum protein, SR-related CTD-associated factor 6, SCAF6
  • Anti-CHERP-ATTO Fluor-594 Antibody
    Anti-CHERP-ATTO Fluor-594 Antibody
    A Rabbit Polyclonal Antibody to CHERP Conjugated to the Fluorescent Dye ATTO-594
    • Product Code: ACC-330-AR
    • Short Description: A Rabbit Polyclonal Antibody to CHERP Conjugated to the Fluorescent Dye ATTO-594
    • Host: Rabbit
    • Label: ATTO 594
    • Reactivity: Human, Rat, Mouse
    • Applications: Immunofluorescence, Immunohistochemistry
    • Target: Calcium homeostasis endoplasmic reticulum protein, SR-related CTD-associated factor 6, SCAF6
  • Anti-CHRM4 Antibody
    Anti-CHRM4 Antibody
    A Rabbit Polyclonal Antibody to Muscarinic Acetylcholine Receptor M4
    • Product Code: AMR-004
    • Short Description: A Rabbit Polyclonal Antibody to Muscarinic Acetylcholine Receptor M4
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: Muscarinic acetylcholine receptor M4, Cholinergic receptor muscarinic 4, mAChR M4, HM4
  • Anti-CHRM5 (extracellular) Antibody
    Anti-CHRM5 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Muscarinic Acetylcholine Receptor M5
    • Product Code: AMR-005
    • Short Description: A Rabbit Polyclonal Antibody to Muscarinic Acetylcholine Receptor M5
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot
    • Target: Muscarinic acetylcholine receptor M5, Cholinergic receptor muscarinic 5, mAChR M5, HM5
  • Anti-CLIC5 Antibody
    KO Validate'
    Anti-CLIC5 Antibody
    A Rabbit Polyclonal Antibody to CLIC5 Channel
    • Product Code: ACL-025
    • Short Description: A Rabbit Polyclonal Antibody to CLIC5 Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: Cl- intracellular channel 5
  • Anti-Connexin-40 (GJA5) Antibody
    Anti-Connexin-40 (GJA5) Antibody
    A Rabbit Polyclonal Antibody to Connexin-40
    • Product Code: ACC-205
    • Short Description: A Rabbit Polyclonal Antibody to Connexin-40
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Western blot
    • Target: CX40, Gap junction α5 protein
  • Anti-Connexin-43 Antibody
    Anti-Connexin-43 Antibody
    A Rabbit Polyclonal Antibody to Connexin-43
    • Product Code: ACC-201
    • Short Description: A Rabbit Polyclonal Antibody to Connexin-43
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: CX43, Gap junction α1 protein, GJA1, GJAL, Gap junction 43 kDa heart protein
  • Anti-CTL1 (SLC44A1) (extracellular) Antibody
    Anti-CTL1 (SLC44A1) (extracellular) Antibody
    A Rabbit Polyclonal antibody to CTL1 (SLC44A1) (extracellular)
    • Product Code: ACT-021
    • Short Description: A Rabbit Polyclonal antibody to CTL1 (SLC44A1) (extracellular)
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot
    • Target: Choline Transporter-Like Protein 1, Solute carrier family 44 member 1, CD92
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