Cardiac Failure

Cardiac Failure

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  • The expected molecular weight (MW) listed in the product datasheet is based solely on the size of the target protein’s amino acid sequence. It’s important to remember that many factors can affect the banding pattern of your western blot including: (1) the existence of a splice variant, (2) the quality of the loaded sample, (3) the protein extraction method, and (4) the protein transfer conditions. To achieve accurate results, you may need to systematically adjust the protein extraction method or the protein transfer conditions.

    • If the band’s MW is below the expected MW it could be due to a splice variant with a slightly different MW.
      • Heat the samples at 70°C for 10 min.
      • Increase the transfer time.
    • If the band’s MW is above the expected MW, it could be due to post-translational modifications.
    • In either event, you should use a blocking peptide as a negative control.

  • Some toxins and peptides are soluble in DMSO; you can find this information in the product specification section. For these products, prepare a concentrated stock solution first.

    1. Centrifuge vial (10,000 x g, 5 minutes) before adding solvent.
    2. Dissolve the lyophilized reagent in DMSO. We recommend keeping a concentrated stock solution at 1-10 mM (100-1000X higher than the final working concentration).
    3. Once the peptide is completely dissolved in DMSO, slowly dilute the peptide in pure water (or buffer) to the desired final working concentration.

    Note: We recommend that you maintain a DMSO concentration as low as possible. For cell assays, a final concentration of 0.1–0.5% DMSO (v/v) is considered acceptable. For other experiments, 5% DMSO (v/v) is recommended; adjust this according to your experimental requirements.

  • FAQ 3FAQ 3FAQ 3FAQ 3

286 Results
  • Anti-OCTN1/SLC22A4 Antibody
    Anti-OCTN1/SLC22A4 Antibody
    A Rabbit Polyclonal antibody to OCTN1/SLC22A4 transporter.
    • Product Code: ACT-014
    • Short Description: A Rabbit Polyclonal antibody to OCTN1/SLC22A4 transporter.
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunohistochemistry
    • Target: Organic Cation/Carnitine Transporter 1, Ergothioneine Transporter, Solute Carrier Family 22 Member 4
  • Anti-Ryanodine Receptor 2 Antibody
    Anti-Ryanodine Receptor 2 Antibody
    A Rabbit Polyclonal Antibody to Ryanodine Receptor 2
    • Product Code: ARR-002
    • Short Description: A Rabbit Polyclonal Antibody to Ryanodine Receptor 2
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: RyR2, Cardiac muscle ryanodine receptor
  • Anti-SCN1B (Na<sub>V</sub>β<sub>1</sub>) (extracellular) Antibody
    Anti-SCN1B (NaVβ1) (extracellular) Antibody
    A Rabbit Polyclonal Antibody to NaVβ1 Subunit
    • Product Code: ASC-041
    • Short Description: A Rabbit Polyclonal Antibody to NaVβ1 Subunit
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Live cell imaging, Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: Voltage-gated sodium channel subunit beta-1, GEFSP1
  • Anti-SCN4B (Na<sub>V</sub>β<sub>4</sub>) (extracellular) Antibody
    Anti-SCN4B (NaVβ4) (extracellular) Antibody
    A Rabbit Polyclonal Antibody to NaVβ4 Subunit
    • Product Code: ASC-044
    • Short Description: A Rabbit Polyclonal Antibody to NaVβ4 Subunit
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: Voltage-gated sodium channel subunit beta-4, LQT10
  • Anti-SERCA2 Antibody
    Anti-SERCA2 Antibody
    A Rabbit Polyclonal Antibody to SERCA2 Pump
    • Product Code: ACP-012
    • Short Description: A Rabbit Polyclonal Antibody to SERCA2 Pump
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunocytochemistry, Immunofluorescence
    • Target: Sarcoplasmic/endoplasmic reticulum calcium ATPase 2, Calcium pump 2, ATP2A2
  • Anti-SGLT2 (extracellular) Antibody
    Anti-SGLT2 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to SGLT2 Transporter
    • Product Code: AGT-032
    • Short Description: A Rabbit Polyclonal Antibody to SGLT2 Transporter
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: Na+/glucose cotransporter 2, Low affinity sodium-glucose cotransporter, SLC5A2
  • Anti-TMEM66 Antibody
    Anti-TMEM66 Antibody
    A Rabbit Polyclonal Antibody to TMEM66
    • Product Code: ACC-067
    • Short Description: A Rabbit Polyclonal Antibody to TMEM66
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: Store-operated calcium entry-associated regulatory factor, SOCE-associated regulatory factor, SARAF, Transmembrane protein 66
  • Anti-TMEM66-ATTO Fluor-594 Antibody
    Anti-TMEM66-ATTO Fluor-594 Antibody
    A Rabbit Polyclonal Antibody to TMEM66 Conjugated to the Fluorescent Dye ATTO-594
    • Product Code: ACC-067-AR
    • Short Description: A Rabbit Polyclonal Antibody to TMEM66 Conjugated to the Fluorescent Dye ATTO-594
    • Host: Rabbit
    • Label: ATTO 594
    • Reactivity: Human, Rat, Mouse
    • Applications: Immunofluorescence, Immunohistochemistry
    • Target: Store-operated calcium entry-associated regulatory factor, SOCE-associated regulatory factor, SARAF, Transmembrane protein 66
  • Anti-TPCN1 Antibody
    Anti-TPCN1 Antibody
    A Rabbit Polyclonal Antibody to Two Pore Calcium Channel Protein 1
    • Product Code: ACC-071
    • Short Description: A Rabbit Polyclonal Antibody to Two Pore Calcium Channel Protein 1
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunohistochemistry
    • Target: Two pore calcium channel protein 1, Voltage-dependent calcium channel protein TPC1, Two pore segment channel 1
  • Anti-TPCN2 Antibody
    KO Validate'
    Anti-TPCN2 Antibody
    A Rabbit Polyclonal Antibody to Two Pore Calcium Channel Protein 2
    • Product Code: ACC-072
    • Short Description: A Rabbit Polyclonal Antibody to Two Pore Calcium Channel Protein 2
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: Two pore calcium channel protein 2, Voltage-dependent calcium channel protein TPC2, SHEP10
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