Genetic Cardiac Diseases

Genetic Cardiac Diseases

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  • The expected molecular weight (MW) listed in the product datasheet is based solely on the size of the target protein’s amino acid sequence. It’s important to remember that many factors can affect the banding pattern of your western blot including: (1) the existence of a splice variant, (2) the quality of the loaded sample, (3) the protein extraction method, and (4) the protein transfer conditions. To achieve accurate results, you may need to systematically adjust the protein extraction method or the protein transfer conditions.

    • If the band’s MW is below the expected MW it could be due to a splice variant with a slightly different MW.
      • Heat the samples at 70°C for 10 min.
      • Increase the transfer time.
    • If the band’s MW is above the expected MW, it could be due to post-translational modifications.
    • In either event, you should use a blocking peptide as a negative control.

  • Some toxins and peptides are soluble in DMSO; you can find this information in the product specification section. For these products, prepare a concentrated stock solution first.

    1. Centrifuge vial (10,000 x g, 5 minutes) before adding solvent.
    2. Dissolve the lyophilized reagent in DMSO. We recommend keeping a concentrated stock solution at 1-10 mM (100-1000X higher than the final working concentration).
    3. Once the peptide is completely dissolved in DMSO, slowly dilute the peptide in pure water (or buffer) to the desired final working concentration.

    Note: We recommend that you maintain a DMSO concentration as low as possible. For cell assays, a final concentration of 0.1–0.5% DMSO (v/v) is considered acceptable. For other experiments, 5% DMSO (v/v) is recommended; adjust this according to your experimental requirements.

  • FAQ 3FAQ 3FAQ 3FAQ 3

132 Results
  • Anti-ENaC γ (SCNN1G) (extracellular) Antibody
    Anti-ENaC γ (SCNN1G) (extracellular) Antibody
    A Rabbit Polyclonal Antibody to ENaC? Channel
    • Product Code: ASC-011
    • Short Description: A Rabbit Polyclonal Antibody to ENaC? Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Mouse
    • Applications: Western blot, Immunohistochemistry
    • Target: Amiloride-sensitive sodium channel subunit gamma, Epithelial sodium channel γ subunit, Gamma-ENaC, Gamma-NaCH, Nonvoltage-gated sodium channel 1 subunit gamma, SCNEG
  • Anti-GIRK3 (K<sub>ir</sub>3.3) Antibody
    KO Validate'
    Anti-GIRK3 (Kir3.3) Antibody
    A Rabbit Polyclonal Antibody to GIRK3 (Kir3.3) Channel
    • Product Code: APC-038
    • Short Description: A Rabbit Polyclonal Antibody to GIRK3 (Kir3.3) Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: G protein-activated inward rectifier potassium channel 3, KCNJ9
  • Anti-GPR126/ADGRG6 (extracellular) Antibody
    Anti-GPR126/ADGRG6 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to GPR126
    • Product Code: AGR-056
    • Short Description: A Rabbit Polyclonal Antibody to GPR126
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot
    • Target: Adhesion G-Protein Coupled Receptor G6, G-Protein Coupled Receptor 126, Developmentally Regulated G-Protein-Coupled Receptor, DREG, Vascular Inducible G Protein-Coupled Receptor, VIGR
  • Anti-GPR35 (extracellular) Antibody
    Anti-GPR35 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to GPR35 (extracellular)
    • Product Code: AGR-051
    • Short Description: A Rabbit Polyclonal Antibody to GPR35 (extracellular)
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunohistochemistry
    • Target: G-Protein Coupled Receptor 35, Kynurenic Acid Receptor
  • Anti-GPR35 (extracellular)-FITC Antibody
    Anti-GPR35 (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to GPR35 (extracellular) conjugated to the fluorescent dye FITC
    • Product Code: AGR-051-F
    • Short Description: A Rabbit Polyclonal Antibody to GPR35 (extracellular) conjugated to the fluorescent dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: G-Protein Coupled Receptor 35, Kynurenic Acid Receptor
  • Anti-GPR97 (ADGRG3)(extracellular) Antibody
    Anti-GPR97 (ADGRG3)(extracellular) Antibody
    A Rabbit Polyclonal antibody to GPR97 (ADGRG3) (extracellular)
    • Product Code: AGR-057
    • Short Description: A Rabbit Polyclonal antibody to GPR97 (ADGRG3) (extracellular)
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunohistochemistry
    • Target: Adhesion G Protein-Coupled Receptor G3, G-protein coupled receptor 97
  • Anti-KCNE4 (MiRP3) Antibody
    Anti-KCNE4 (MiRP3) Antibody
    A Rabbit Polyclonal Antibody to KCNE4 (MiRP3) Subunit
    • Product Code: APC-129
    • Short Description: A Rabbit Polyclonal Antibody to KCNE4 (MiRP3) Subunit
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot
    • Target: Potassium channel subunit β MiRP3, MinK-related peptide 3, Potassium voltage-gated channel subfamily E member 4
  • Anti-KCNF1 (K<sub>V</sub>5.1) Antibody
    Anti-KCNF1 (KV5.1) Antibody
    A Rabbit Polyclonal Antibody to KCNF1 (KV5.1) Channel
    • Product Code: APC-134
    • Short Description: A Rabbit Polyclonal Antibody to KCNF1 (KV5.1) Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot
    • Target: Potassium voltage-gated channel subfamily F member 1
  • Anti-KCNH7 (erg3) Antibody
    Anti-KCNH7 (erg3) Antibody
    A Rabbit Polyclonal Antibody to KV11.3 (ERG-3) Channel
    • Product Code: APC-112
    • Short Description: A Rabbit Polyclonal Antibody to KV11.3 (ERG-3) Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: KV11.3, Potassium voltage-gated channel subfamily H member 7, Ether-a-go-go-related channel 3, Eag-related protein 3, ERG-3, hERG3
  • Anti-KCNJ1 (K<sub>ir</sub>1.1) Antibody
    KO Validate'
    Anti-KCNJ1 (Kir1.1) Antibody
    A Rabbit Polyclonal Antibody to KCNJ1 (Kir1.1) Channel
    • Product Code: APC-001
    • Short Description: A Rabbit Polyclonal Antibody to KCNJ1 (Kir1.1) Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry
    • Target: ROMK1, ATP-sensitive inward rectifier potassium channel 1
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