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  • The expected molecular weight (MW) listed in the product datasheet is based solely on the size of the target protein’s amino acid sequence. It’s important to remember that many factors can affect the banding pattern of your western blot including: (1) the existence of a splice variant, (2) the quality of the loaded sample, (3) the protein extraction method, and (4) the protein transfer conditions. To achieve accurate results, you may need to systematically adjust the protein extraction method or the protein transfer conditions.

    • If the band’s MW is below the expected MW it could be due to a splice variant with a slightly different MW.
      • Heat the samples at 70°C for 10 min.
      • Increase the transfer time.
    • If the band’s MW is above the expected MW, it could be due to post-translational modifications.
    • In either event, you should use a blocking peptide as a negative control.
  • Some toxins and peptides are soluble in DMSO; you can find this information in the product specification section. For these products, prepare a concentrated stock solution first.

    1. Centrifuge vial (10,000 x g, 5 minutes) before adding solvent.
    2. Dissolve the lyophilized reagent in DMSO. We recommend keeping a concentrated stock solution at 1-10 mM (100-1000X higher than the final working concentration).
    3. Once the peptide is completely dissolved in DMSO, slowly dilute the peptide in pure water (or buffer) to the desired final working concentration.

    Note: We recommend that you maintain a DMSO concentration as low as possible. For cell assays, a final concentration of 0.1–0.5% DMSO (v/v) is considered acceptable. For other experiments, 5% DMSO (v/v) is recommended; adjust this according to your experimental requirements.

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561 Results
  • Anti-TRPP1 (PKD2) Antibody
    A Rabbit Polyclonal Antibody to TRPP1 (PKD2) Channel
    • Product Code: ACC-052
    • Short Description: A Rabbit Polyclonal Antibody to TRPP1 (PKD2) Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: Polycystin-2, Polycystic kidney disease 2 protein, PC2
  • Anti-TRPV4 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to TRPV4 Channel
    • Product Code: ACC-124
    • Short Description: A Rabbit Polyclonal Antibody to TRPV4 Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: OTRPC4, VRL-2, VR-OAC, TRP12
  • Anti-TRPV4 (extracellular) Antibody- PE Antibody
    A Rabbit Polyclonal Antibody to TRPV4 (extracellular) conjugated to the fluorescent dye R-Phycoerythrin
    • Product Code: ACC-124-PE
    • Short Description: A Rabbit Polyclonal Antibody to TRPV4 (extracellular) conjugated to the fluorescent dye R-Phycoerythrin
    • Host: Rabbit
    • Label: R-PE
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: OTRPC4, VRL-2, VR-OAC, TRP12, Osm-9-Like TRP Channel 4
  • Anti-TRPV4 Antibody
    A Rabbit Polyclonal Antibody to TRPV4 Channel
    • Product Code: ACC-034
    • Short Description: A Rabbit Polyclonal Antibody to TRPV4 Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Western blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry
    • Target: OTRPC4, VRL-2, VR-OAC, TRP12
  • Anti-TRPV4-ATTO Fluor-550 Antibody
    A Rabbit Polyclonal Antibody to TRPV4 Channel Conjugated to the Fluorescent Dye ATTO-550
    • Product Code: ACC-034-AO
    • Short Description: A Rabbit Polyclonal Antibody to TRPV4 Channel Conjugated to the Fluorescent Dye ATTO-550
    • Host: Rabbit
    • Label: ATTO 550
    • Reactivity: Human, Rat, Mouse
    • Applications: Immunofluorescence, Immunohistochemistry
    • Target: OTRPC4, VRL-2, VR-OAC, TRP12
  • Anti-TRPV5 Antibody
    A Rabbit Polyclonal Antibody to TRPV5 Channel
    • Product Code: ACC-035
    • Short Description: A Rabbit Polyclonal Antibody to TRPV5 Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: Transient receptor potential cation channel subfamily V member 5, Calcium transport protein 2, CaT2, Epithelial calcium channel 1, ECaC1, OTRPC3
  • Anti-TRPV6 Antibody
    A Rabbit Polyclonal Antibody to TRPV6 Channel
    • Product Code: ACC-036
    • Short Description: A Rabbit Polyclonal Antibody to TRPV6 Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry
    • Target: Epithelial calcium channel 2, ECaC2, Calcium transport protein 1, CaT1, CaT-like, CaT-L, Transient receptor potential cation channel subfamily V member 6
  • Anti-UCP2 Antibody
    A Rabbit Polyclonal Antibody to Mitochondrial Uncoupling Protein 2
    • Product Code: ANT-128
    • Short Description: A Rabbit Polyclonal Antibody to Mitochondrial Uncoupling Protein 2
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: Mitochondrial uncoupling protein 2, UCPH, Solute carrier family 25 member 8, SLC25A8, BMIQ4
  • Anti-Urotensin II Receptor Antibody
    A Rabbit Ployclonal Antibody to Urotensin II Receptor
    • Product Code: AER-003
    • Short Description: A Rabbit Ployclonal Antibody to Urotensin II Receptor
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Mouse
    • Applications: Western blot, Immunohistochemistry
    • Target: Urotensin 2 receptor, UR-II-R, UR2R, UTS2R, SENR, GPR14
  • Anti-Vasopressin V<sub>2</sub> Receptor (AVPR2) Antibody
    A Rabbit Polyclonal Antibody to Vasopressin V2 Receptor
    • Product Code: AVR-012
    • Short Description: A Rabbit Polyclonal Antibody to Vasopressin V2 Receptor
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Western blot
    • Target: V2R, AVPR V2, Antidiuretic hormone receptor, ADHR, Renal-type arginine vasopressin receptor, DIR3, Nephrogenic diabetes insipidus
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