Hypertension

Hypertension

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  • The expected molecular weight (MW) listed in the product datasheet is based solely on the size of the target protein’s amino acid sequence. It’s important to remember that many factors can affect the banding pattern of your western blot including: (1) the existence of a splice variant, (2) the quality of the loaded sample, (3) the protein extraction method, and (4) the protein transfer conditions. To achieve accurate results, you may need to systematically adjust the protein extraction method or the protein transfer conditions.

    • If the band’s MW is below the expected MW it could be due to a splice variant with a slightly different MW.
      • Heat the samples at 70°C for 10 min.
      • Increase the transfer time.
    • If the band’s MW is above the expected MW, it could be due to post-translational modifications.
    • In either event, you should use a blocking peptide as a negative control.

  • Some toxins and peptides are soluble in DMSO; you can find this information in the product specification section. For these products, prepare a concentrated stock solution first.

    1. Centrifuge vial (10,000 x g, 5 minutes) before adding solvent.
    2. Dissolve the lyophilized reagent in DMSO. We recommend keeping a concentrated stock solution at 1-10 mM (100-1000X higher than the final working concentration).
    3. Once the peptide is completely dissolved in DMSO, slowly dilute the peptide in pure water (or buffer) to the desired final working concentration.

    Note: We recommend that you maintain a DMSO concentration as low as possible. For cell assays, a final concentration of 0.1–0.5% DMSO (v/v) is considered acceptable. For other experiments, 5% DMSO (v/v) is recommended; adjust this according to your experimental requirements.

  • FAQ 3FAQ 3FAQ 3FAQ 3

561 Results
  • Anti-Glucagon Receptor (extracellular) Antibody
    Anti-Glucagon Receptor (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Glucagon Receptor
    • Product Code: AGR-024
    • Short Description: A Rabbit Polyclonal Antibody to Glucagon Receptor
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: GCGR, GLR
  • Anti-GPR120/FFAR4 (extracellular) Antibody
    Anti-GPR120/FFAR4 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Free Fatty Acid Receptor 4 (GPR120)
    • Product Code: AFR-014
    • Short Description: A Rabbit Polyclonal Antibody to Free Fatty Acid Receptor 4 (GPR120)
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunohistochemistry
    • Target: G-protein coupled receptor 120, Free fatty acid receptor 4, FFA4 Receptor, G-protein coupled receptor GT01, Omega-3 fatty acid receptor 1, O3far1
  • Anti-GPR120/FFAR4 (extracellular)-FITC Antibody
    Anti-GPR120/FFAR4 (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to GPR120/FFAR4 Conjugated to the Fluorescent Dye FITC
    • Product Code: AFR-014-F
    • Short Description: A Rabbit Polyclonal Antibody to GPR120/FFAR4 Conjugated to the Fluorescent Dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Human, Rat, Mouse
    • Applications: Flow cytometry
    • Target: G-protein coupled receptor 120, Free fatty acid receptor 4, FFA4 Receptor, G-protein coupled receptor GT01, Omega-3 fatty acid receptor 1, O3far1
  • Anti-GPR40/FFAR1 (extracellular) Antibody
    Anti-GPR40/FFAR1 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Free Fatty Acid Receptor 1 (GPR40)
    • Product Code: AFR-011
    • Short Description: A Rabbit Polyclonal Antibody to Free Fatty Acid Receptor 1 (GPR40)
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunofluorescence, Immunohistochemistry
    • Target: G-protein coupled receptor 40, Free fatty acid receptor 1
  • Anti-GPR43/FFAR2 Antibody
    Anti-GPR43/FFAR2 Antibody
    A Rabbit Polyclonal Antibody to Free Fatty Acid Receptor 2 (GPR43)
    • Product Code: AFR-032
    • Short Description: A Rabbit Polyclonal Antibody to Free Fatty Acid Receptor 2 (GPR43)
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Western blot, Immunohistochemistry
    • Target: G-protein coupled receptor 43, Free fatty acid receptor 2
  • Anti-GPR91 (SUCNR1) (extracellular) Antibody
    Anti-GPR91 (SUCNR1) (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Succinate Receptor 1
    • Product Code: ASR-090
    • Short Description: A Rabbit Polyclonal Antibody to Succinate Receptor 1
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunohistochemistry
    • Target: G-protein coupled receptor 91, Succinate receptor 1
  • Anti-GPR91 (SUCNR1) (extracellular)-FITC Antibody
    Anti-GPR91 (SUCNR1) (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to GPR91 Conjugated to the Fluorescent Dye FITC
    • Product Code: ASR-090-F
    • Short Description: A Rabbit Polyclonal Antibody to GPR91 Conjugated to the Fluorescent Dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: G-protein coupled receptor 91, Succinate receptor 1
  • Anti-GPR91 (SUCNR1) (extracellular)-PE Antibody
    Anti-GPR91 (SUCNR1) (extracellular)-PE Antibody
    A Rabbit Polyclonal Antibody to GPR91 (SUCNR1) (extracellular) conjugated to the fluorescent dye Phycoerythrin (PE)
    • Product Code: ASR-090-PE
    • Short Description: A Rabbit Polyclonal Antibody to GPR91 (SUCNR1) (extracellular) conjugated to the fluorescent dye Phycoerythrin (PE)
    • Host: Rabbit
    • Label: R-PE
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: G-protein coupled receptor 91, Succinate receptor 1
  • Anti-HCN4 Antibody
    KO Validate'
    Anti-HCN4 Antibody
    A Rabbit Polyclonal Antibody to HCN4 Channel
    • Product Code: APC-052
    • Short Description: A Rabbit Polyclonal Antibody to HCN4 Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry
    • Target: Hyperpolarization-activated cyclic nucleotide-gated potassium channel 4
  • Anti-Human ABCB1/MDR1 Antibody
    Anti-Human ABCB1/MDR1 Antibody
    A Rabbit Polyclonal Antibody to Multidrug Resistance Protein 1
    • Product Code: AMT-021
    • Short Description: A Rabbit Polyclonal Antibody to Multidrug Resistance Protein 1
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human
    • Applications: Western blot
    • Target: Multidrug resistance protein 1, ATP-binding cassette sub-family B (MDR/TAP) member 1, P-glycoprotein 1, P-gp, PGY1, ABC20, CD243, CLCS, Colchicin sensitivity, Doxorubicin resistance, GP170
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