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  • The expected molecular weight (MW) listed in the product datasheet is based solely on the size of the target protein’s amino acid sequence. It’s important to remember that many factors can affect the banding pattern of your western blot including: (1) the existence of a splice variant, (2) the quality of the loaded sample, (3) the protein extraction method, and (4) the protein transfer conditions. To achieve accurate results, you may need to systematically adjust the protein extraction method or the protein transfer conditions.

    • If the band’s MW is below the expected MW it could be due to a splice variant with a slightly different MW.
      • Heat the samples at 70°C for 10 min.
      • Increase the transfer time.
    • If the band’s MW is above the expected MW, it could be due to post-translational modifications.
    • In either event, you should use a blocking peptide as a negative control.
  • Some toxins and peptides are soluble in DMSO; you can find this information in the product specification section. For these products, prepare a concentrated stock solution first.

    1. Centrifuge vial (10,000 x g, 5 minutes) before adding solvent.
    2. Dissolve the lyophilized reagent in DMSO. We recommend keeping a concentrated stock solution at 1-10 mM (100-1000X higher than the final working concentration).
    3. Once the peptide is completely dissolved in DMSO, slowly dilute the peptide in pure water (or buffer) to the desired final working concentration.

    Note: We recommend that you maintain a DMSO concentration as low as possible. For cell assays, a final concentration of 0.1–0.5% DMSO (v/v) is considered acceptable. For other experiments, 5% DMSO (v/v) is recommended; adjust this according to your experimental requirements.

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561 Results
  • Anti-Leptin Receptor (extracellular)-FITC Antibody
    A Rabbit Polyclonal antibody to Leptin Receptor (extracellular) conjugated to the fluorescent dye FITC.
    • Product Code: ALR-039-F
    • Short Description: A Rabbit Polyclonal antibody to Leptin Receptor (extracellular) conjugated to the fluorescent dye FITC.
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: LEPR, OB receptor, OBR, CD295
  • Anti-LRRC8A (extracellular) Antibody
    A Rabbit Polyclonal Antibody to LRRC8A
    • Product Code: AAC-001
    • Short Description: A Rabbit Polyclonal Antibody to LRRC8A
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunofluorescence
    • Target: Leucine-rich repeat-containing protein 8A, Volume-regulated anion channel subunit LRRC8A, LRRC8, Swelling protein 1, SWELL1
  • Anti-MC2 Receptor (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Melanocortin Receptor 2
    • Product Code: AMR-022
    • Short Description: A Rabbit Polyclonal Antibody to Melanocortin Receptor 2
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunohistochemistry
    • Target: Melanocortin receptor 2, MC2R, MC2-R, Adrenocorticotropic hormone receptor, ACTH receptor, ACTHR, ACTH-R, Adrenocorticotropin receptor
  • Anti-MC3 Receptor (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Melanocortin Receptor 3
    • Product Code: AMR-023
    • Short Description: A Rabbit Polyclonal Antibody to Melanocortin Receptor 3
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunofluorescence, Immunohistochemistry
    • Target: Melanocortin receptor 3, MC3R, MC3-R
  • Anti-MC4 Receptor (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Melanocortin Receptor 4
    • Product Code: AMR-024
    • Short Description: A Rabbit Polyclonal Antibody to Melanocortin Receptor 4
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: Melanocortin receptor 4, MC4R, MC4-R
  • Anti-Melatonin Receptor 1B (MTNR1B) Antibody
    A Rabbit Polyclonal Antibody to Melatonin Receptor Type 1B
    • Product Code: AMR-032
    • Short Description: A Rabbit Polyclonal Antibody to Melatonin Receptor Type 1B
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: Melatonin receptor type 2, MT2, Mel1b receptor, Mel-1B-R
  • Anti-MRS2 Antibody
    A Rabbit Polyclonal Antibody to MRS2 Magnesium Transporter
    • Product Code: ANT-148
    • Short Description: A Rabbit Polyclonal Antibody to MRS2 Magnesium Transporter
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunohistochemistry
    • Target: MRS2 magnesium transporter, Magnesium transporter MRS2 homolog mitochondrial, MRS2-like protein, MRS2L
  • Anti-N-Cadherin Antibody
    A Rabbit Polyclonal Antibody to N-Cadherin
    • Product Code: ANR-082
    • Short Description: A Rabbit Polyclonal Antibody to N-Cadherin
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunohistochemistry
    • Target: Cadherin 2, Neural cadherin, CDH2, NCAD, CDHN, CD325, Calcium-dependent adhesion protein neuronal
  • Anti-N-Cadherin-ATTO Fluor-594 Antibody
    A Rabbit Polyclonal Antibody to N-Cadherin Conjugated to the Fluorescent Dye ATTO-594
    • Product Code: ANR-082-AR
    • Short Description: A Rabbit Polyclonal Antibody to N-Cadherin Conjugated to the Fluorescent Dye ATTO-594
    • Host: Rabbit
    • Label: ATTO 594
    • Reactivity: Human, Rat, Mouse
    • Applications: Immunofluorescence, Immunohistochemistry
    • Target: Cadherin 2, Neural cadherin, CDH2, NCAD, CDHN, CD325, Calcium-dependent adhesion protein neuronal
  • Anti-Na<sup>+</sup>/H<sup>+</sup> Exchanger 3 (NHE-3) Antibody
    A Rabbit Polyclonal Antibody to Na+/H+ Exchanger 3 (NHE-3)
    • Product Code: ANX-033
    • Short Description: A Rabbit Polyclonal Antibody to Na+/H+ Exchanger 3 (NHE-3)
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Mouse
    • Applications: Western blot
    • Target: Sodium/hydrogen exchanger 3, SLC9A3
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