Hypertension

Hypertension

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  • The expected molecular weight (MW) listed in the product datasheet is based solely on the size of the target protein’s amino acid sequence. It’s important to remember that many factors can affect the banding pattern of your western blot including: (1) the existence of a splice variant, (2) the quality of the loaded sample, (3) the protein extraction method, and (4) the protein transfer conditions. To achieve accurate results, you may need to systematically adjust the protein extraction method or the protein transfer conditions.

    • If the band’s MW is below the expected MW it could be due to a splice variant with a slightly different MW.
      • Heat the samples at 70°C for 10 min.
      • Increase the transfer time.
    • If the band’s MW is above the expected MW, it could be due to post-translational modifications.
    • In either event, you should use a blocking peptide as a negative control.

  • Some toxins and peptides are soluble in DMSO; you can find this information in the product specification section. For these products, prepare a concentrated stock solution first.

    1. Centrifuge vial (10,000 x g, 5 minutes) before adding solvent.
    2. Dissolve the lyophilized reagent in DMSO. We recommend keeping a concentrated stock solution at 1-10 mM (100-1000X higher than the final working concentration).
    3. Once the peptide is completely dissolved in DMSO, slowly dilute the peptide in pure water (or buffer) to the desired final working concentration.

    Note: We recommend that you maintain a DMSO concentration as low as possible. For cell assays, a final concentration of 0.1–0.5% DMSO (v/v) is considered acceptable. For other experiments, 5% DMSO (v/v) is recommended; adjust this according to your experimental requirements.

  • FAQ 3FAQ 3FAQ 3FAQ 3

561 Results
  • Anti-Orai1 (extracellular) Antibody
    KO Validate'
    Anti-Orai1 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Orai1 Channel
    • Product Code: ACC-062
    • Short Description: A Rabbit Polyclonal Antibody to Orai1 Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: TMEM142A, CRACM1, Calcium release-activated calcium channel protein 1
  • Anti-Orai1 (extracellular)-ATTO Fluor-488 Antibody
    Anti-Orai1 (extracellular)-ATTO Fluor-488 Antibody
    A Rabbit Polyclonal Antibody to Orai1 Channel Conjugated to the Fluorescent Dye ATTO-488
    • Product Code: ACC-062-AG
    • Short Description: A Rabbit Polyclonal Antibody to Orai1 Channel Conjugated to the Fluorescent Dye ATTO-488
    • Host: Rabbit
    • Label: ATTO 488
    • Reactivity: Rat, Mouse
    • Applications: Immunocytochemistry, Immunofluorescence
    • Target: TMEM142A, CRACM1, Calcium release-activated calcium channel protein 1
  • Anti-Orai1 (extracellular)-FITC Antibody
    Anti-Orai1 (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to Orai1 (extracellular) conjugated to the fluorescent dye FITC.
    • Product Code: ACC-062-F
    • Short Description: A Rabbit Polyclonal Antibody to Orai1 (extracellular) conjugated to the fluorescent dye FITC.
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: TMEM142A, Calcium release-activated calcium channel protein 1, CRACM1
  • Anti-Orai2 Antibody
    Anti-Orai2 Antibody
    A Rabbit Polyclonal Antibody to Orai2 Channel
    • Product Code: ACC-061
    • Short Description: A Rabbit Polyclonal Antibody to Orai2 Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: TMEM142B
  • Anti-Orai3 Antibody
    Anti-Orai3 Antibody
    A Rabbit Polyclonal Antibody to Orai3 Channel
    • Product Code: ACC-065
    • Short Description: A Rabbit Polyclonal Antibody to Orai3 Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot
    • Target: TMEM142C
  • Anti-Piezo1 (extracellular) Antibody
    KO Validate'
    Anti-Piezo1 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Piezo1 Channel
    • Product Code: APC-087
    • Short Description: A Rabbit Polyclonal Antibody to Piezo1 Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunofluorescence, Immunohistochemistry
    • Target: Piezo-type mechanosensitive ion channel component 1, Protein FAM38A, Mib, Membrane protein induced by beta-amyloid treatment
  • Anti-Piezo1 (extracellular)-APC Antibody
    Anti-Piezo1 (extracellular)-APC Antibody
    A Rabbit Polyclonal Antibody to Piezo1 (extracellular) conjugated to the fluorescent dye Allophycocyanin (APC)
    • Product Code: APC-087-APC
    • Short Description: A Rabbit Polyclonal Antibody to Piezo1 (extracellular) conjugated to the fluorescent dye Allophycocyanin (APC)
    • Host: Rabbit
    • Label: APC
    • Reactivity: Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: Piezo-type mechanosensitive ion channel component 1, Protein FAM38A, Mib, Membrane protein induced by beta-amyloid treatment
  • Anti-Piezo1 (extracellular)-FITC Antibody
    Anti-Piezo1 (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to Piezo1 (extracellular) conjugated to the fluorescent dye FITC
    • Product Code: APC-087-F
    • Short Description: A Rabbit Polyclonal Antibody to Piezo1 (extracellular) conjugated to the fluorescent dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: Piezo-type mechanosensitive ion channel component 1, Protein FAM38A, Mib, Membrane protein induced by beta-amyloid treatment
  • Anti-Piezo2 Antibody
    KO Validate'
    Anti-Piezo2 Antibody
    A Rabbit Polyclonal Antibody to Piezo2
    • Product Code: APC-090
    • Short Description: A Rabbit Polyclonal Antibody to Piezo2
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunohistochemistry
    • Target: Piezo-Type Mechanosensitive Ion Channel Component 2, Protein FAM38B
  • Anti-PMCA1 Antibody
    Anti-PMCA1 Antibody
    A Rabbit Polyclonal Antibody to PMCA1 ATPase
    • Product Code: ACP-005
    • Short Description: A Rabbit Polyclonal Antibody to PMCA1 ATPase
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: Plasma membrane Ca2+ ATPase 1, ATP2B1
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