Hypertension

Hypertension

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  • The expected molecular weight (MW) listed in the product datasheet is based solely on the size of the target protein’s amino acid sequence. It’s important to remember that many factors can affect the banding pattern of your western blot including: (1) the existence of a splice variant, (2) the quality of the loaded sample, (3) the protein extraction method, and (4) the protein transfer conditions. To achieve accurate results, you may need to systematically adjust the protein extraction method or the protein transfer conditions.

    • If the band’s MW is below the expected MW it could be due to a splice variant with a slightly different MW.
      • Heat the samples at 70°C for 10 min.
      • Increase the transfer time.
    • If the band’s MW is above the expected MW, it could be due to post-translational modifications.
    • In either event, you should use a blocking peptide as a negative control.

  • Some toxins and peptides are soluble in DMSO; you can find this information in the product specification section. For these products, prepare a concentrated stock solution first.

    1. Centrifuge vial (10,000 x g, 5 minutes) before adding solvent.
    2. Dissolve the lyophilized reagent in DMSO. We recommend keeping a concentrated stock solution at 1-10 mM (100-1000X higher than the final working concentration).
    3. Once the peptide is completely dissolved in DMSO, slowly dilute the peptide in pure water (or buffer) to the desired final working concentration.

    Note: We recommend that you maintain a DMSO concentration as low as possible. For cell assays, a final concentration of 0.1–0.5% DMSO (v/v) is considered acceptable. For other experiments, 5% DMSO (v/v) is recommended; adjust this according to your experimental requirements.

  • FAQ 3FAQ 3FAQ 3FAQ 3

561 Results
  • Anti-Adenosine A<sub>2B</sub> Receptor (extracellular) Antibody
    KO Validate'
    Anti-Adenosine A2B Receptor (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Adenosine A2B Receptor
    • Product Code: AAR-003
    • Short Description: A Rabbit Polyclonal Antibody to Adenosine A2B Receptor
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: ADORA2b, A2BAR, Adenosine A2bR
  • Anti-Adenosine A<sub>2B</sub> Receptor (extracellular)-APC Antibody
    Anti-Adenosine A2B Receptor (extracellular)-APC Antibody
    A Rabbit Polyclonal Antibody to Adenosine A2B Receptor (extracellular) conjugated to the fluorescent dye Allophycocyanin (APC)
    • Product Code: AAR-003-APC
    • Short Description: A Rabbit Polyclonal Antibody to Adenosine A2B Receptor (extracellular) conjugated to the fluorescent dye Allophycocyanin (APC)
    • Host: Rabbit
    • Label: APC
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: ADORA2b, A2BAR, Adenosine A2b Receptor
  • Anti-Adenosine A<sub>2B</sub> Receptor (extracellular)-FITC Antibody
    Anti-Adenosine A2B Receptor (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to Adenosine A2B Receptor Conjugated to the Fluorescent Dye FITC
    • Product Code: AAR-003-F
    • Short Description: A Rabbit Polyclonal Antibody to Adenosine A2B Receptor Conjugated to the Fluorescent Dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Human, Rat
    • Applications: Flow cytometry
    • Target: ADORA2b, A2BAR, Adenosine A2bR
  • Anti-Adenosine A<sub>3</sub> Receptor Antibody
    Anti-Adenosine A3 Receptor Antibody
    A Rabbit Polyclonal Antibody to Adenosine A3 Receptor
    • Product Code: AAR-004
    • Short Description: A Rabbit Polyclonal Antibody to Adenosine A3 Receptor
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Mouse
    • Applications: Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: ADORA3, A3AR, Adenosine A3R
  • Anti-Adenylate Cyclase 3 (AC3) (extracellular) Antibody
    Anti-Adenylate Cyclase 3 (AC3) (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Adenylate Cyclase Type III
    • Product Code: AAR-043
    • Short Description: A Rabbit Polyclonal Antibody to Adenylate Cyclase Type III
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: Adenylate cyclase type 3, Adenylate cyclase type III, AC-III, ATP pyrophosphate-lyase 3, Adenylate cyclase olfactive type, Adenylyl cyclase 3, ADCY3
  • Anti-Adenylate Cyclase 3 (AC3) (extracellular)-ATTO Fluor-488 Antibody
    Anti-Adenylate Cyclase 3 (AC3) (extracellular)-ATTO Fluor-488 Antibody
    A Rabbit Polyclonal Antibody to Adenylate Cyclase Type III Conjugated to the Fluorescent Dye ATTO-488
    • Product Code: AAR-043-AG
    • Short Description: A Rabbit Polyclonal Antibody to Adenylate Cyclase Type III Conjugated to the Fluorescent Dye ATTO-488
    • Host: Rabbit
    • Label: ATTO 488
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Immunocytochemistry, Immunofluorescence, Flow cytometry
    • Target: Adenylate cyclase type 3, Adenylate cyclase type III, AC-III, ATP pyrophosphate-lyase 3, Adenylate cyclase olfactive type, Adenylyl cyclase 3, ADCY3
  • Anti-AKAP12 Antibody
    Anti-AKAP12 Antibody
    A Rabbit Polyclonal Antibody to AKAP12
    • Product Code: APZ-033
    • Short Description: A Rabbit Polyclonal Antibody to AKAP12
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: A-kinase anchoring protein 12, A-kinase anchor protein 250 kDa, AKAP250, Gravin, Myasthenia gravis autoantigen gravin, Src-suppressed C kinase substrate, SSeCKS
  • Anti-Alpha 1 Na<sup>+</sup>/K<sup>+</sup> ATPase Antibody
    Anti-Alpha 1 Na+/K+ ATPase Antibody
    A Rabbit Polyclonal Antibody to Alpha 1 Na+/K+ ATPase
    • Product Code: ANP-001
    • Short Description: A Rabbit Polyclonal Antibody to Alpha 1 Na+/K+ ATPase
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: ATP1A1, Sodium/potassium ATPase catalytic subunit alpha-1, Sodium pump subunit alpha-1
  • Anti-Angiotensin II Receptor Type-1 (extracellular) Antibody
    Anti-Angiotensin II Receptor Type-1 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Angiotensin II Receptor Type-1
    • Product Code: AAR-011
    • Short Description: A Rabbit Polyclonal Antibody to Angiotensin II Receptor Type-1
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: AT1 Receptor, AT1R, AGTR1
  • Anti-Angiotensin II Receptor Type-1 (extracellular)-ATTO Fluor-550 Antibody
    Anti-Angiotensin II Receptor Type-1 (extracellular)-ATTO Fluor-550 Antibody
    A Rabbit Polyclonal Antibody to Angiotensin II Receptor Type-1 Conjugated to the Fluorescent Dye ATTO-550
    • Product Code: AAR-011-AO
    • Short Description: A Rabbit Polyclonal Antibody to Angiotensin II Receptor Type-1 Conjugated to the Fluorescent Dye ATTO-550
    • Host: Rabbit
    • Label: ATTO 550
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: AT1 Receptor, AT1R, AGTR1
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