Hypertension

Hypertension

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  • The expected molecular weight (MW) listed in the product datasheet is based solely on the size of the target protein’s amino acid sequence. It’s important to remember that many factors can affect the banding pattern of your western blot including: (1) the existence of a splice variant, (2) the quality of the loaded sample, (3) the protein extraction method, and (4) the protein transfer conditions. To achieve accurate results, you may need to systematically adjust the protein extraction method or the protein transfer conditions.

    • If the band’s MW is below the expected MW it could be due to a splice variant with a slightly different MW.
      • Heat the samples at 70°C for 10 min.
      • Increase the transfer time.
    • If the band’s MW is above the expected MW, it could be due to post-translational modifications.
    • In either event, you should use a blocking peptide as a negative control.

  • Some toxins and peptides are soluble in DMSO; you can find this information in the product specification section. For these products, prepare a concentrated stock solution first.

    1. Centrifuge vial (10,000 x g, 5 minutes) before adding solvent.
    2. Dissolve the lyophilized reagent in DMSO. We recommend keeping a concentrated stock solution at 1-10 mM (100-1000X higher than the final working concentration).
    3. Once the peptide is completely dissolved in DMSO, slowly dilute the peptide in pure water (or buffer) to the desired final working concentration.

    Note: We recommend that you maintain a DMSO concentration as low as possible. For cell assays, a final concentration of 0.1–0.5% DMSO (v/v) is considered acceptable. For other experiments, 5% DMSO (v/v) is recommended; adjust this according to your experimental requirements.

  • FAQ 3FAQ 3FAQ 3FAQ 3

561 Results
  • Anti-ENaC γ (SCNN1G) (extracellular) Antibody
    Anti-ENaC γ (SCNN1G) (extracellular) Antibody
    A Rabbit Polyclonal Antibody to ENaC? Channel
    • Product Code: ASC-011
    • Short Description: A Rabbit Polyclonal Antibody to ENaC? Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Mouse
    • Applications: Western blot, Immunohistochemistry
    • Target: Amiloride-sensitive sodium channel subunit gamma, Epithelial sodium channel γ subunit, Gamma-ENaC, Gamma-NaCH, Nonvoltage-gated sodium channel 1 subunit gamma, SCNEG
  • Anti-Endothelin Receptor A (extracellular) Antibody
    Anti-Endothelin Receptor A (extracellular) Antibody
    A Rabbit Ployclonal Antibody to Endothelin Receptor A
    • Product Code: AER-004
    • Short Description: A Rabbit Ployclonal Antibody to Endothelin Receptor A
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot
    • Target: EDNRA, ET-A, ET-AR, ETRA, ET1-Specific type endothelin receptor, Endothelin-1 receptor, Endothelin Receptor Type A
  • Anti-Endothelin Receptor A Antibody
    Anti-Endothelin Receptor A Antibody
    A Rabbit Polyclonal Antibody to Endothelin Receptor A
    • Product Code: AER-001
    • Short Description: A Rabbit Polyclonal Antibody to Endothelin Receptor A
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: EDNRA, ETA, ET-AR, ETRA, ET1-Specific type endothelin receptor, Endothelin-1 receptor
  • Anti-Endothelin Receptor B (extracellular) Antibody
    Anti-Endothelin Receptor B (extracellular) Antibody
    A Rabbit Polyclonal antibody to Endothelin Receptor B (extracellular)
    • Product Code: AER-005
    • Short Description: A Rabbit Polyclonal antibody to Endothelin Receptor B (extracellular)
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot
    • Target: EDNRB, ET-B, ETB, ETRB, Endothelin B receptor, ETBR
  • Anti-Endothelin Receptor B Antibody
    Anti-Endothelin Receptor B Antibody
    A Rabbit Polyclonal Antibody to Endothelin Receptor B
    • Product Code: AER-002
    • Short Description: A Rabbit Polyclonal Antibody to Endothelin Receptor B
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: EDNRB, ET-B, ETB, ETRB, Endothelin B receptor, Endothelin receptor non-selective type
  • Anti-EphB4 (extracellular) Antibody
    Anti-EphB4 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to EphB4 Receptor
    • Product Code: AER-024
    • Short Description: A Rabbit Polyclonal Antibody to EphB4 Receptor
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunofluorescence, Immunohistochemistry
    • Target: Ephrin type-B receptor 4, Eph receptor B4, Hepatoma transmembrane kinase, HTK
  • Anti-GIPR (extracellular) Antibody
    Anti-GIPR (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Gastric Inhibitory Polypeptide Receptor
    • Product Code: AGR-025
    • Short Description: A Rabbit Polyclonal Antibody to Gastric Inhibitory Polypeptide Receptor
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunofluorescence, Immunohistochemistry
    • Target: Gastric inhibitory polypeptide receptor, GIP receptor, Glucose-dependent insulinotropic polypeptide receptor
  • Anti-GLP1R (extracellular) Antibody
    KO Validate'
    Anti-GLP1R (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Glucagon-like Peptide 1 Receptor
    • Product Code: AGR-021
    • Short Description: A Rabbit Polyclonal Antibody to Glucagon-like Peptide 1 Receptor
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: Glucagon-like peptide 1 receptor, GLP-1 receptor
  • Anti-GLP1R (extracellular)-FITC Antibody
    Anti-GLP1R (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to Glucagon-like Peptide 1 Receptor Conjugated to the Fluorescent Dye FITC
    • Product Code: AGR-021-F
    • Short Description: A Rabbit Polyclonal Antibody to Glucagon-like Peptide 1 Receptor Conjugated to the Fluorescent Dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: Glucagon-like peptide 1 receptor, GLP-1 receptor
  • Anti-GLP1R (extracellular)-PE Antibody
    Anti-GLP1R (extracellular)-PE Antibody
    A Rabbit Polyclonal Antibody to GLP1R conjugated to R-Phycoerythrin (R-PE) fluorophore
    • Product Code: AGR-021-PE
    • Short Description: A Rabbit Polyclonal Antibody to GLP1R conjugated to R-Phycoerythrin (R-PE) fluorophore
    • Host: Rabbit
    • Label: R-PE
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: Glucagon-like peptide 1 receptor, GLP-1 receptor
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