Thrombosis and Atherosclerosis

Thrombosis and Atherosclerosis

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  • The expected molecular weight (MW) listed in the product datasheet is based solely on the size of the target protein’s amino acid sequence. It’s important to remember that many factors can affect the banding pattern of your western blot including: (1) the existence of a splice variant, (2) the quality of the loaded sample, (3) the protein extraction method, and (4) the protein transfer conditions. To achieve accurate results, you may need to systematically adjust the protein extraction method or the protein transfer conditions.

    • If the band’s MW is below the expected MW it could be due to a splice variant with a slightly different MW.
      • Heat the samples at 70°C for 10 min.
      • Increase the transfer time.
    • If the band’s MW is above the expected MW, it could be due to post-translational modifications.
    • In either event, you should use a blocking peptide as a negative control.

  • Some toxins and peptides are soluble in DMSO; you can find this information in the product specification section. For these products, prepare a concentrated stock solution first.

    1. Centrifuge vial (10,000 x g, 5 minutes) before adding solvent.
    2. Dissolve the lyophilized reagent in DMSO. We recommend keeping a concentrated stock solution at 1-10 mM (100-1000X higher than the final working concentration).
    3. Once the peptide is completely dissolved in DMSO, slowly dilute the peptide in pure water (or buffer) to the desired final working concentration.

    Note: We recommend that you maintain a DMSO concentration as low as possible. For cell assays, a final concentration of 0.1–0.5% DMSO (v/v) is considered acceptable. For other experiments, 5% DMSO (v/v) is recommended; adjust this according to your experimental requirements.

  • FAQ 3FAQ 3FAQ 3FAQ 3

239 Results
  • Anti-PAR1 (F2R) (extracellular) Antibody
    Anti-PAR1 (F2R) (extracellular) Antibody
    A Rabbit Polyclonal Antibody to PAR1 (F2R) (extracellular)
    • Product Code: APR-036
    • Short Description: A Rabbit Polyclonal Antibody to PAR1 (F2R) (extracellular)
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunohistochemistry
    • Target: Proteinase-activated receptor 1, Coagulation factor II receptor, CF2R
  • Anti-PAR1 (F2R) (extracellular)-FITC Antibody
    Anti-PAR1 (F2R) (extracellular)-FITC Antibody
    A Rabbit polyclonal antibody to PAR1 (F2R) (extracellular) conjugated to fluorescein isothiocyanate (FITC) fluorophore.
    • Product Code: APR-036-F
    • Short Description: A Rabbit polyclonal antibody to PAR1 (F2R) (extracellular) conjugated to fluorescein isothiocyanate (FITC) fluorophore.
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: Proteinase-activated receptor 1, Coagulation factor II receptor, CF2R
  • Anti-PAR2 (F2RL1) Antibody
    Anti-PAR2 (F2RL1) Antibody
    A Rabbit Polyclonal Antibody to Protease-Activated Receptor-2
    • Product Code: APR-032
    • Short Description: A Rabbit Polyclonal Antibody to Protease-Activated Receptor-2
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: Protease-activated receptor-2, PAR-2, Thrombin receptor-like 1, Coagulation factor II receptor-like 1, GPR11
  • Anti-PAR4 (F2RL3) (extracellular) Antibody
    KO Validate'
    Anti-PAR4 (F2RL3) (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Protease-Activated Receptor-4
    • Product Code: APR-034
    • Short Description: A Rabbit Polyclonal Antibody to Protease-Activated Receptor-4
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: Protease-activated receptor-4, PAR-4, Coagulation factor II receptor-like 3, Thrombin receptor-like 3
  • Anti-PAR4 (F2RL3) (extracellular)-APC Antibody
    Anti-PAR4 (F2RL3) (extracellular)-APC Antibody
    A Rabbit Polyclonal Antibody to PAR4 (F2RL3) (extracellular) conjugated to the fluorescent dye Allophycocyanin (APC)
    • Product Code: APR-034-APC
    • Short Description: A Rabbit Polyclonal Antibody to PAR4 (F2RL3) (extracellular) conjugated to the fluorescent dye Allophycocyanin (APC)
    • Host: Rabbit
    • Label: APC
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: Protease-activated receptor-4, PAR-4, Coagulation factor II receptor-like 3, Thrombin receptor-like 3
  • Anti-PAR4 (F2RL3) (extracellular)-FITC Antibody
    KO Validate'
    Anti-PAR4 (F2RL3) (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to Protease-Activated Receptor-4 Conjugated to the Fluorescent Dye FITC
    • Product Code: APR-034-F
    • Short Description: A Rabbit Polyclonal Antibody to Protease-Activated Receptor-4 Conjugated to the Fluorescent Dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: Protease-activated receptor-4, PAR-4, Coagulation factor II receptor-like 3, Thrombin receptor-like 3
  • Anti-Platelet-activating factor receptor (extracellular) Antibody
    Anti-Platelet-activating factor receptor (extracellular) Antibody
    A Rabbit Polyclonal antibody to Platelet-activating factor receptor (extracellular)
    • Product Code: APR-055
    • Short Description: A Rabbit Polyclonal antibody to Platelet-activating factor receptor (extracellular)
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunohistochemistry
    • Target: PTAFR, PAFR
  • Anti-Platelet-activating factor receptor (extracellular)-FITC Antibody
    Anti-Platelet-activating factor receptor (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to Platelet-activating factor receptor (extracellular) conjugated to the fluorescent dye FITC
    • Product Code: APR-055-F
    • Short Description: A Rabbit Polyclonal Antibody to Platelet-activating factor receptor (extracellular) conjugated to the fluorescent dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: PTAFR, PAFR
  • Anti-Prokineticin Receptor 1 (extracellular) Antibody
    Anti-Prokineticin Receptor 1 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Prokineticin Receptor 1
    • Product Code: APR-041
    • Short Description: A Rabbit Polyclonal Antibody to Prokineticin Receptor 1
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunofluorescence, Immunohistochemistry
    • Target: PROKR1, PKR1, G-protein coupled receptor 73, Gpr73, Gpr73a, G-protein coupled receptor ZAQ
  • Anti-Prokineticin Receptor 2 (extracellular) Antibody
    KO Validate'
    Anti-Prokineticin Receptor 2 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Prokineticin Receptor 2
    • Product Code: APR-042
    • Short Description: A Rabbit Polyclonal Antibody to Prokineticin Receptor 2
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunofluorescence, Immunohistochemistry
    • Target: PROKR2, PKR2, G-protein coupled receptor 73-like 1, Gpr73l1, Gpr73b, G-protein coupled receptor I5E
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