Thrombosis and Atherosclerosis

Thrombosis and Atherosclerosis

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  • The expected molecular weight (MW) listed in the product datasheet is based solely on the size of the target protein’s amino acid sequence. It’s important to remember that many factors can affect the banding pattern of your western blot including: (1) the existence of a splice variant, (2) the quality of the loaded sample, (3) the protein extraction method, and (4) the protein transfer conditions. To achieve accurate results, you may need to systematically adjust the protein extraction method or the protein transfer conditions.

    • If the band’s MW is below the expected MW it could be due to a splice variant with a slightly different MW.
      • Heat the samples at 70°C for 10 min.
      • Increase the transfer time.
    • If the band’s MW is above the expected MW, it could be due to post-translational modifications.
    • In either event, you should use a blocking peptide as a negative control.

  • Some toxins and peptides are soluble in DMSO; you can find this information in the product specification section. For these products, prepare a concentrated stock solution first.

    1. Centrifuge vial (10,000 x g, 5 minutes) before adding solvent.
    2. Dissolve the lyophilized reagent in DMSO. We recommend keeping a concentrated stock solution at 1-10 mM (100-1000X higher than the final working concentration).
    3. Once the peptide is completely dissolved in DMSO, slowly dilute the peptide in pure water (or buffer) to the desired final working concentration.

    Note: We recommend that you maintain a DMSO concentration as low as possible. For cell assays, a final concentration of 0.1–0.5% DMSO (v/v) is considered acceptable. For other experiments, 5% DMSO (v/v) is recommended; adjust this according to your experimental requirements.

  • FAQ 3FAQ 3FAQ 3FAQ 3

239 Results
  • Anti-Endothelin Receptor A Antibody
    Anti-Endothelin Receptor A Antibody
    A Rabbit Polyclonal Antibody to Endothelin Receptor A
    • Product Code: AER-001
    • Short Description: A Rabbit Polyclonal Antibody to Endothelin Receptor A
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: EDNRA, ETA, ET-AR, ETRA, ET1-Specific type endothelin receptor, Endothelin-1 receptor
  • Anti-EphA2 (extracellular) Antibody
    Anti-EphA2 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to EphA2 Receptor
    • Product Code: AER-019
    • Short Description: A Rabbit Polyclonal Antibody to EphA2 Receptor
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunohistochemistry
    • Target: Ephrin type A receptor 2, EPH Receptor A2, Epithelial Cell Kinase, Tyrosine-Protein Kinase Receptor ECK, ECK, EPHA2
  • Anti-EphB4 (extracellular) Antibody
    Anti-EphB4 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to EphB4 Receptor
    • Product Code: AER-024
    • Short Description: A Rabbit Polyclonal Antibody to EphB4 Receptor
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunofluorescence, Immunohistochemistry
    • Target: Ephrin type-B receptor 4, Eph receptor B4, Hepatoma transmembrane kinase, HTK
  • Anti-Ephrin-A1 (extracellular) Antibody
    Anti-Ephrin-A1 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Ephrin-A1
    • Product Code: AER-031
    • Short Description: A Rabbit Polyclonal Antibody to Ephrin-A1
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunocytochemistry, Immunofluorescence
    • Target: EPH-related receptor tyrosine kinase ligand 1, LERK1, EFNA1
  • Anti-GPR120/FFAR4 (extracellular) Antibody
    Anti-GPR120/FFAR4 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Free Fatty Acid Receptor 4 (GPR120)
    • Product Code: AFR-014
    • Short Description: A Rabbit Polyclonal Antibody to Free Fatty Acid Receptor 4 (GPR120)
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunohistochemistry
    • Target: G-protein coupled receptor 120, Free fatty acid receptor 4, FFA4 Receptor, G-protein coupled receptor GT01, Omega-3 fatty acid receptor 1, O3far1
  • Anti-GPR120/FFAR4 (extracellular)-FITC Antibody
    Anti-GPR120/FFAR4 (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to GPR120/FFAR4 Conjugated to the Fluorescent Dye FITC
    • Product Code: AFR-014-F
    • Short Description: A Rabbit Polyclonal Antibody to GPR120/FFAR4 Conjugated to the Fluorescent Dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Human, Rat, Mouse
    • Applications: Flow cytometry
    • Target: G-protein coupled receptor 120, Free fatty acid receptor 4, FFA4 Receptor, G-protein coupled receptor GT01, Omega-3 fatty acid receptor 1, O3far1
  • Anti-GPR43/FFAR2 Antibody
    Anti-GPR43/FFAR2 Antibody
    A Rabbit Polyclonal Antibody to Free Fatty Acid Receptor 2 (GPR43)
    • Product Code: AFR-032
    • Short Description: A Rabbit Polyclonal Antibody to Free Fatty Acid Receptor 2 (GPR43)
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Western blot, Immunohistochemistry
    • Target: G-protein coupled receptor 43, Free fatty acid receptor 2
  • Anti-Human BLT1 (extracellular) Antibody
    Anti-Human BLT1 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to BLT1
    • Product Code: ALR-001
    • Short Description: A Rabbit Polyclonal Antibody to BLT1
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human
    • Applications: Indirect flow cytometry, Western blot
    • Target: Leukotriene B4 receptor 1, LTB4R, LTB4R1, CMKRL1, GPR16, P2Y7, P2RY7
  • Anti-Human BLT1 (extracellular)-ATTO Fluor-488 Antibody
    Anti-Human BLT1 (extracellular)-ATTO Fluor-488 Antibody
    A Rabbit Polyclonal Antibody to BLT1 Conjugated to the Fluorescent Dye ATTO-488
    • Product Code: ALR-001-AG
    • Short Description: A Rabbit Polyclonal Antibody to BLT1 Conjugated to the Fluorescent Dye ATTO-488
    • Host: Rabbit
    • Label: ATTO 488
    • Reactivity: Human
    • Applications: Flow cytometry
    • Target: Leukotriene B4 receptor 1, LTB4R, LTB4R1, CMKRL1, GPR16, P2Y7, P2RY7
  • Anti-Human CCR2 (extracellular) Antibody
    Anti-Human CCR2 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to CCR2 Receptor
    • Product Code: ACR-022
    • Short Description: A Rabbit Polyclonal Antibody to CCR2 Receptor
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot
    • Target: C-C chemokine receptor type 2, Monocyte chemoattractant protein 1 receptor, MCP-1 receptor, CC-CKR-2, CD192
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