Thrombosis and Atherosclerosis

Thrombosis and Atherosclerosis

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  • The expected molecular weight (MW) listed in the product datasheet is based solely on the size of the target protein’s amino acid sequence. It’s important to remember that many factors can affect the banding pattern of your western blot including: (1) the existence of a splice variant, (2) the quality of the loaded sample, (3) the protein extraction method, and (4) the protein transfer conditions. To achieve accurate results, you may need to systematically adjust the protein extraction method or the protein transfer conditions.

    • If the band’s MW is below the expected MW it could be due to a splice variant with a slightly different MW.
      • Heat the samples at 70°C for 10 min.
      • Increase the transfer time.
    • If the band’s MW is above the expected MW, it could be due to post-translational modifications.
    • In either event, you should use a blocking peptide as a negative control.

  • Some toxins and peptides are soluble in DMSO; you can find this information in the product specification section. For these products, prepare a concentrated stock solution first.

    1. Centrifuge vial (10,000 x g, 5 minutes) before adding solvent.
    2. Dissolve the lyophilized reagent in DMSO. We recommend keeping a concentrated stock solution at 1-10 mM (100-1000X higher than the final working concentration).
    3. Once the peptide is completely dissolved in DMSO, slowly dilute the peptide in pure water (or buffer) to the desired final working concentration.

    Note: We recommend that you maintain a DMSO concentration as low as possible. For cell assays, a final concentration of 0.1–0.5% DMSO (v/v) is considered acceptable. For other experiments, 5% DMSO (v/v) is recommended; adjust this according to your experimental requirements.

  • FAQ 3FAQ 3FAQ 3FAQ 3

239 Results
  • Anti-Human CCR3 (extracellular) Antibody
    Anti-Human CCR3 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to CCR3 Chemokine Receptor
    • Product Code: ACR-023
    • Short Description: A Rabbit Polyclonal Antibody to CCR3 Chemokine Receptor
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot
    • Target: C-C chemokine receptor type 3, Eosinophil eotaxin receptor, CKR3, C-C CKR-3, CMKBR3, B-chemokine receptor, CD193 antigen
  • Anti-Human CCR5 (extracellular) Antibody
    Anti-Human CCR5 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to CCR5 Chemokine Receptor
    • Product Code: ACR-025
    • Short Description: A Rabbit Polyclonal Antibody to CCR5 Chemokine Receptor
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot
    • Target: C-C chemokine receptor type 5, C-C CKR-5, CHEMR13, HIV-1 fusion coreceptor, CD195
  • Anti-Human CXCR1 (extracellular) Antibody
    Anti-Human CXCR1 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to CXCR1 Chemokine Receptor
    • Product Code: ACR-011
    • Short Description: A Rabbit Polyclonal Antibody to CXCR1 Chemokine Receptor
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot
    • Target: C-X-C chemokine receptor type 1, Interleukin-8 receptor A, IL-8R A, IL8RA, IL-8 receptor type 1, CD181
  • Anti-Human CXCR2 (extracellular) Antibody
    Anti-Human CXCR2 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to CXCR2 Chemokine Receptor
    • Product Code: ACR-012
    • Short Description: A Rabbit Polyclonal Antibody to CXCR2 Chemokine Receptor
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot
    • Target: C-X-C chemokine receptor type 2, Interleukin-8 receptor B, IL-8R B, IL8RB, IL-8 receptor type 2, CD182
  • Anti-Human CXCR2 (extracellular)-ATTO Fluor-488 Antibody
    Anti-Human CXCR2 (extracellular)-ATTO Fluor-488 Antibody
    A Rabbit Polyclonal Antibody to CXCR2 Chemokine Receptor Conjugated to the Fluorescent Dye ATTO-488
    • Product Code: ACR-012-AG
    • Short Description: A Rabbit Polyclonal Antibody to CXCR2 Chemokine Receptor Conjugated to the Fluorescent Dye ATTO-488
    • Host: Rabbit
    • Label: ATTO 488
    • Reactivity: Human
    • Applications: Live cell imaging, Flow cytometry
    • Target: C-X-C chemokine receptor type 2, Interleukin-8 receptor B, IL-8R B, IL8RB, IL-8 receptor type 2, CD182
  • Anti-Human FPR1 (extracellular) Antibody
    Anti-Human FPR1 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Human FPR1
    • Product Code: AFR-001
    • Short Description: A Rabbit Polyclonal Antibody to Human FPR1
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot
    • Target: N-formyl peptide receptor 1, fMLP receptor
  • Anti-Human FPR1 (extracellular)-ATTO Fluor-488 Antibody
    Anti-Human FPR1 (extracellular)-ATTO Fluor-488 Antibody
    A Rabbit Polyclonal Antibody to FPR1 Conjugated to the Fluorescent Dye ATTO-488
    • Product Code: AFR-001-AG
    • Short Description: A Rabbit Polyclonal Antibody to FPR1 Conjugated to the Fluorescent Dye ATTO-488
    • Host: Rabbit
    • Label: ATTO 488
    • Reactivity: Human
    • Applications: Live cell imaging, Flow cytometry
    • Target: N-formyl peptide receptor 1, fMLP receptor
  • Anti-Human FPR2/ALX (extracellular) Antibody
    Anti-Human FPR2/ALX (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Human FPR2/ALX
    • Product Code: AFR-002
    • Short Description: A Rabbit Polyclonal Antibody to Human FPR2/ALX
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunofluorescence, Immunohistochemistry
    • Target: N-formyl peptide receptor 2, FMLP-related receptor I, FMLPX, Formyl peptide receptor-like 1, FPRL1, ALXR, Lipoxin A4 receptor, LXA4 receptor, LXA4R, RFP
  • Anti-Human GPR43/FFAR2 (extracellular) Antibody
    Anti-Human GPR43/FFAR2 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Human Free Fatty Acid Receptor 2 (GPR43)
    • Product Code: AFR-015
    • Short Description: A Rabbit Polyclonal Antibody to Human Free Fatty Acid Receptor 2 (GPR43)
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot
    • Target: G-protein coupled receptor 43, Free fatty acid receptor 2
  • Anti-Human P2Y11 Receptor Antibody
    Anti-Human P2Y11 Receptor Antibody
    A Rabbit Polyclonal Antibody to P2Y11 Receptor
    • Product Code: APR-015
    • Short Description: A Rabbit Polyclonal Antibody to P2Y11 Receptor
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human
    • Applications: Indirect flow cytometry, Western blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
    • Target: P2Y purinoceptor 11, P2RY11
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