Thrombosis and Atherosclerosis

Thrombosis and Atherosclerosis

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  • The expected molecular weight (MW) listed in the product datasheet is based solely on the size of the target protein’s amino acid sequence. It’s important to remember that many factors can affect the banding pattern of your western blot including: (1) the existence of a splice variant, (2) the quality of the loaded sample, (3) the protein extraction method, and (4) the protein transfer conditions. To achieve accurate results, you may need to systematically adjust the protein extraction method or the protein transfer conditions.

    • If the band’s MW is below the expected MW it could be due to a splice variant with a slightly different MW.
      • Heat the samples at 70°C for 10 min.
      • Increase the transfer time.
    • If the band’s MW is above the expected MW, it could be due to post-translational modifications.
    • In either event, you should use a blocking peptide as a negative control.

  • Some toxins and peptides are soluble in DMSO; you can find this information in the product specification section. For these products, prepare a concentrated stock solution first.

    1. Centrifuge vial (10,000 x g, 5 minutes) before adding solvent.
    2. Dissolve the lyophilized reagent in DMSO. We recommend keeping a concentrated stock solution at 1-10 mM (100-1000X higher than the final working concentration).
    3. Once the peptide is completely dissolved in DMSO, slowly dilute the peptide in pure water (or buffer) to the desired final working concentration.

    Note: We recommend that you maintain a DMSO concentration as low as possible. For cell assays, a final concentration of 0.1–0.5% DMSO (v/v) is considered acceptable. For other experiments, 5% DMSO (v/v) is recommended; adjust this according to your experimental requirements.

  • FAQ 3FAQ 3FAQ 3FAQ 3

239 Results
  • Anti-Human PAR1 (F2R) (extracellular) Antibody
    Anti-Human PAR1 (F2R) (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Protease-Activated Receptor-1
    • Product Code: APR-031
    • Short Description: A Rabbit Polyclonal Antibody to Protease-Activated Receptor-1
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: Protease-activated receptor-1, PAR-1, Thrombin receptor, Coagulation factor II receptor, CF2R
  • Anti-Human PAR1 (F2R) (extracellular)-FITC Antibody
    Anti-Human PAR1 (F2R) (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to Protease-Activated Receptor-1 Conjugated to the Fluorescent dye FITC
    • Product Code: APR-031-F
    • Short Description: A Rabbit Polyclonal Antibody to Protease-Activated Receptor-1 Conjugated to the Fluorescent dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Human
    • Applications: Live cell imaging, Flow cytometry
    • Target: Protease-activated receptor-1, PAR-1, Thrombin receptor, Coagulation factor II receptor, CF2R
  • Anti-Human PAR2/F2RL1 (extracellular) Antibody
    Anti-Human PAR2/F2RL1 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Protease-Activated Receptor-2
    • Product Code: APR-035
    • Short Description: A Rabbit Polyclonal Antibody to Protease-Activated Receptor-2
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot
    • Target: Protease-activated receptor-2, PAR-2, Thrombin receptor-like 1, Coagulation factor II receptor-like 1, GPR11
  • Anti-Human PAR2/F2RL1 (extracellular)-FITC Antibody
    Anti-Human PAR2/F2RL1 (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to PAR2 (extracellular) conjugated to the fluorescent dye FITC
    • Product Code: APR-035-F
    • Short Description: A Rabbit Polyclonal Antibody to PAR2 (extracellular) conjugated to the fluorescent dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Human
    • Applications: Live cell imaging, Flow cytometry
    • Target: Protease-activated receptor-2, PAR-2, Thrombin receptor-like 1, Coagulation factor II receptor-like 1, GPR11
  • Anti-KCNK3 (TASK-1) Antibody
    Anti-KCNK3 (TASK-1) Antibody
    A Rabbit Polyclonal Antibody to KCNK3 Channel
    • Product Code: APC-024
    • Short Description: A Rabbit Polyclonal Antibody to KCNK3 Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Mouse
    • Applications: Western blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry
    • Target: Potassium channel subfamily K member 3, TWIK-related acid sensitive K+ channel, K2P3.1, cTBAK-1, OAT1
  • Anti-Neuropilin-1 (NRP1) (extracellular) Antibody
    Anti-Neuropilin-1 (NRP1) (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Neuropilin-1
    • Product Code: ANR-063
    • Short Description: A Rabbit Polyclonal Antibody to Neuropilin-1
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunocytochemistry, Immunofluorescence
    • Target: Vascular endothelial cell growth factor 165 receptor, VEGF165R, CD304
  • Anti-Neuropilin-1 (NRP1) (extracellular)-FITC Antibody
    Anti-Neuropilin-1 (NRP1) (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to Neuropilin-1 (NRP1) (extracellular) conjugated to the fluorescent dye FITC.
    • Product Code: ANR-063-F
    • Short Description: A Rabbit Polyclonal Antibody to Neuropilin-1 (NRP1) (extracellular) conjugated to the fluorescent dye FITC.
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Human, Rat, Mouse
    • Applications: Flow cytometry
    • Target: Vascular endothelial cell growth factor 165 receptor, VEGF165R, CD304
  • Anti-Neuropilin-2 (NRP2) (extracellular) Antibody
    Anti-Neuropilin-2 (NRP2) (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Neuropilin-2
    • Product Code: ANR-062
    • Short Description: A Rabbit Polyclonal Antibody to Neuropilin-2
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Western blot, Immunofluorescence, Immunohistochemistry
    • Target: Vascular endothelial cell growth factor 165 receptor 2, VEGF165R2
  • Anti-Neuropilin-2 (NRP2) (extracellular)-FITC Antibody
    Anti-Neuropilin-2 (NRP2) (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to Neuropilin-2 Conjugated to the Fluorescent Dye FITC
    • Product Code: ANR-062-F
    • Short Description: A Rabbit Polyclonal Antibody to Neuropilin-2 Conjugated to the Fluorescent Dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Human, Rat, Mouse
    • Applications: Flow cytometry
    • Target: Vascular endothelial cell growth factor 165 receptor 2, VEGF165R2
  • Anti-P2X1 Receptor (extracellular) Antibody
    Anti-P2X1 Receptor (extracellular) Antibody
    A Rabbit Polyclonal Antibody to P2X1 Receptor
    • Product Code: APR-022
    • Short Description: A Rabbit Polyclonal Antibody to P2X1 Receptor
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: P2RX1, P2X purinoceptor 1
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