Thrombosis and Atherosclerosis

Thrombosis and Atherosclerosis

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  • The expected molecular weight (MW) listed in the product datasheet is based solely on the size of the target protein’s amino acid sequence. It’s important to remember that many factors can affect the banding pattern of your western blot including: (1) the existence of a splice variant, (2) the quality of the loaded sample, (3) the protein extraction method, and (4) the protein transfer conditions. To achieve accurate results, you may need to systematically adjust the protein extraction method or the protein transfer conditions.

    • If the band’s MW is below the expected MW it could be due to a splice variant with a slightly different MW.
      • Heat the samples at 70°C for 10 min.
      • Increase the transfer time.
    • If the band’s MW is above the expected MW, it could be due to post-translational modifications.
    • In either event, you should use a blocking peptide as a negative control.

  • Some toxins and peptides are soluble in DMSO; you can find this information in the product specification section. For these products, prepare a concentrated stock solution first.

    1. Centrifuge vial (10,000 x g, 5 minutes) before adding solvent.
    2. Dissolve the lyophilized reagent in DMSO. We recommend keeping a concentrated stock solution at 1-10 mM (100-1000X higher than the final working concentration).
    3. Once the peptide is completely dissolved in DMSO, slowly dilute the peptide in pure water (or buffer) to the desired final working concentration.

    Note: We recommend that you maintain a DMSO concentration as low as possible. For cell assays, a final concentration of 0.1–0.5% DMSO (v/v) is considered acceptable. For other experiments, 5% DMSO (v/v) is recommended; adjust this according to your experimental requirements.

  • FAQ 3FAQ 3FAQ 3FAQ 3

239 Results
  • Anti-Prostacyclin Receptor (PTGIR) (extracellular) Antibody
    Anti-Prostacyclin Receptor (PTGIR) (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Prostacyclin Receptor (PTGIR)
    • Product Code: APR-068
    • Short Description: A Rabbit Polyclonal Antibody to Prostacyclin Receptor (PTGIR)
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot
    • Target: Prostanoid IP receptor, Prostaglandin I2 receptor, PGI2 receptor
  • Anti-Prostacyclin Receptor (PTGIR) (extracellular)-FITC Antibody
    Anti-Prostacyclin Receptor (PTGIR) (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to PTGIR (extracellular) conjugated to the fluorescent dye FITC
    • Product Code: APR-068-F
    • Short Description: A Rabbit Polyclonal Antibody to PTGIR (extracellular) conjugated to the fluorescent dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: Prostanoid IP receptor, Prostaglandin I2 receptor, PGI2 receptor, PRIPR
  • Anti-Prostaglandin F2-α Receptor/PTGFR Antibody
    Anti-Prostaglandin F2-α Receptor/PTGFR Antibody
    A Rabbit Polyclonal Antibody to Prostaglandin F2-α Receptor
    • Product Code: APR-067
    • Short Description: A Rabbit Polyclonal Antibody to Prostaglandin F2-α Receptor
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: Prostaglandin F2-alpha receptor, Prostaglandin F receptor, Prostanoid FP receptor, PGF2-alpha receptor
  • Anti-PrRP Receptor (GPR10) Antibody
    Anti-PrRP Receptor (GPR10) Antibody
    A Rabbit Polyclonal Antibody to Prolactin-Releasing Peptide Receptor
    • Product Code: APR-054
    • Short Description: A Rabbit Polyclonal Antibody to Prolactin-Releasing Peptide Receptor
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Western blot
    • Target: Prolactin-releasing peptide receptor, PrRPR, Prolactin-releasing hormone receptor, PRLHR
  • Anti-S1PR1 (EDG1) (extracellular) Antibody
    Anti-S1PR1 (EDG1) (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Sphingosine 1-Phosphate Receptor 1
    • Product Code: ASR-011
    • Short Description: A Rabbit Polyclonal Antibody to Sphingosine 1-Phosphate Receptor 1
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: Sphingosine 1-phosphate receptor 1, S1P1, Endothelial differentiation G-protein coupled receptor 1, CD363, CHEDG1
  • Anti-S1PR1 (EDG1) (extracellular)-FITC Antibody
    Anti-S1PR1 (EDG1) (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to Sphingosine 1-Phosphate Receptor 1 Conjugated to the Fluorescent Dye FITC
    • Product Code: ASR-011-F
    • Short Description: A Rabbit Polyclonal Antibody to Sphingosine 1-Phosphate Receptor 1 Conjugated to the Fluorescent Dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: Sphingosine 1-phosphate receptor 1, S1P1, Endothelial differentiation G-protein coupled receptor 1, CHEDG1, CD363
  • Anti-S1PR2 (EDG5) Antibody
    Anti-S1PR2 (EDG5) Antibody
    A Rabbit Polyclonal Antibody to Sphingosine 1-Phosphate Receptor 2
    • Product Code: ASR-012
    • Short Description: A Rabbit Polyclonal Antibody to Sphingosine 1-Phosphate Receptor 2
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunohistochemistry
    • Target: Sphingosine 1-phosphate receptor 2, S1P2, Endothelial differentiation G-protein coupled receptor 5, Lysophospholipid receptor B2, LPB2
  • Anti-S1PR3 (EDG3) (extracellular) Antibody
    Anti-S1PR3 (EDG3) (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Sphingosine 1-Phosphate Receptor 3
    • Product Code: ASR-013
    • Short Description: A Rabbit Polyclonal Antibody to Sphingosine 1-Phosphate Receptor 3
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot
    • Target: Sphingosine 1-phosphate receptor 3, S1P3, Endothelial differentiation G-protein coupled receptor 3, Lysophospholipid receptor B3, LPB3
  • Anti-S1PR3 (EDG3) (extracellular)-ATTO Fluor-488 Antibody
    Anti-S1PR3 (EDG3) (extracellular)-ATTO Fluor-488 Antibody
    A Rabbit Polyclonal Antibody to Sphingosine 1-Phosphate Receptor 3 Conjugated to the Fluorescent Dye ATTO-488
    • Product Code: ASR-013-AG
    • Short Description: A Rabbit Polyclonal Antibody to Sphingosine 1-Phosphate Receptor 3 Conjugated to the Fluorescent Dye ATTO-488
    • Host: Rabbit
    • Label: ATTO 488
    • Reactivity: Human, Rat, Mouse
    • Applications: Flow cytometry
    • Target: Sphingosine 1-phosphate receptor 3, S1P3, Endothelial differentiation G-protein coupled receptor 3, Lysophospholipid receptor B3, LPB3
  • Anti-S1PR3 (EDG3) (extracellular)-FITC Antibody
    Anti-S1PR3 (EDG3) (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to Sphingosine 1-Phosphate Receptor 3 Conjugated to the Fluorescent Dye FITC
    • Product Code: ASR-013-F
    • Short Description: A Rabbit Polyclonal Antibody to Sphingosine 1-Phosphate Receptor 3 Conjugated to the Fluorescent Dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: Sphingosine 1-phosphate receptor 3, S1P3, Endothelial differentiation G-protein coupled receptor 3, Lysophospholipid receptor B3, LPB3
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