Thrombosis and Atherosclerosis

Thrombosis and Atherosclerosis

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  • The expected molecular weight (MW) listed in the product datasheet is based solely on the size of the target protein’s amino acid sequence. It’s important to remember that many factors can affect the banding pattern of your western blot including: (1) the existence of a splice variant, (2) the quality of the loaded sample, (3) the protein extraction method, and (4) the protein transfer conditions. To achieve accurate results, you may need to systematically adjust the protein extraction method or the protein transfer conditions.

    • If the band’s MW is below the expected MW it could be due to a splice variant with a slightly different MW.
      • Heat the samples at 70°C for 10 min.
      • Increase the transfer time.
    • If the band’s MW is above the expected MW, it could be due to post-translational modifications.
    • In either event, you should use a blocking peptide as a negative control.

  • Some toxins and peptides are soluble in DMSO; you can find this information in the product specification section. For these products, prepare a concentrated stock solution first.

    1. Centrifuge vial (10,000 x g, 5 minutes) before adding solvent.
    2. Dissolve the lyophilized reagent in DMSO. We recommend keeping a concentrated stock solution at 1-10 mM (100-1000X higher than the final working concentration).
    3. Once the peptide is completely dissolved in DMSO, slowly dilute the peptide in pure water (or buffer) to the desired final working concentration.

    Note: We recommend that you maintain a DMSO concentration as low as possible. For cell assays, a final concentration of 0.1–0.5% DMSO (v/v) is considered acceptable. For other experiments, 5% DMSO (v/v) is recommended; adjust this according to your experimental requirements.

  • FAQ 3FAQ 3FAQ 3FAQ 3

239 Results
  • Angiotensin II
    Bioassay Tested
    Angiotensin II
    A potent agonist for AT1 and AT2 G-protein coupled receptors
    • Product Code: GPA-100
    • Short Description: A potent agonist for AT1 and AT2 G-protein coupled receptors
    • Applications: Aequorin functional assay, Western blot, Calcium imaging assay
    • CAS No.: 4474-91-3
    • MW: 1046.2 Da
  • Angiotensin II acetate
    Bioassay Tested
    Angiotensin II acetate
    A potent agonist for AT1 and AT2 G-protein coupled receptors
    • Product Code: SPA-165
    • Short Description: A potent agonist for AT1 and AT2 G-protein coupled receptors
    • Applications: Aequorin functional assay, Calcium imaging assay
    • CAS No.: 68521-88-0
    • MW: 1046 Da
  • Angiotensin II Receptor Type-2 (extracellular) Blocking Peptide
    Angiotensin II Receptor Type-2 (extracellular) Blocking Peptide
    A Blocking Peptide for Anti-Angiotensin II Receptor Type-2 (extracellular) Antibody
    • Product Code: BLP-AR012
    • Short Description: A Blocking Peptide for Anti-Angiotensin II Receptor Type-2 (extracellular) Antibody
    • Applications: Western blot, Immunohistochemistry
    • Target: AGTR2
  • Angiotensin III
    Bioassay Tested
    Angiotensin III
    A potent agonist for AT1 and AT2 G-protein coupled receptors
    • Product Code: GPA-130
    • Short Description: A potent agonist for AT1 and AT2 G-protein coupled receptors
    • Applications: Aequorin functional assay, Western blot, Calcium imaging assay
    • CAS No.: 13602-53-4
    • MW: 931.1 Da
  • Anthopleurin-C
    Bioassay Tested
    Anthopleurin-C
    A potent cardiotoxin that delays the inactivation of NaV channels
    • Product Code: STA-400
    • Short Description: A potent cardiotoxin that delays the inactivation of NaV channels
    • Applications: Electrophysiology
    • MW: 4877.6 Da
  • Anti-ACE2 (extracellular) Antibody
    Anti-ACE2 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to ACE2 (extracellular)
    • Product Code: AAC-012
    • Short Description: A Rabbit Polyclonal Antibody to ACE2 (extracellular)
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunohistochemistry
    • Target: Angiotensin-Converting Enzyme 2, ACE-Related Carboxypeptidase, Angiotensin-converting enzyme homolog, ACEH, Metalloprotease MPROT15
  • Anti-ACE2 (extracellular)-FITC Antibody
    Anti-ACE2 (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to ACE2 (extracellular) conjugated to the fluorescent dye FITC
    • Product Code: AAC-012-F
    • Short Description: A Rabbit Polyclonal Antibody to ACE2 (extracellular) conjugated to the fluorescent dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: Angiotensin-Converting Enzyme 2, ACE-Related Carboxypeptidase, Angiotensin-converting enzyme homolog, ACEH, Metalloprotease MPROT15
  • Anti-Adenosine A<sub>2B</sub> Receptor (extracellular) Antibody
    KO Validate'
    Anti-Adenosine A2B Receptor (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Adenosine A2B Receptor
    • Product Code: AAR-003
    • Short Description: A Rabbit Polyclonal Antibody to Adenosine A2B Receptor
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: ADORA2b, A2BAR, Adenosine A2bR
  • Anti-Adenosine A<sub>2B</sub> Receptor (extracellular)-APC Antibody
    Anti-Adenosine A2B Receptor (extracellular)-APC Antibody
    A Rabbit Polyclonal Antibody to Adenosine A2B Receptor (extracellular) conjugated to the fluorescent dye Allophycocyanin (APC)
    • Product Code: AAR-003-APC
    • Short Description: A Rabbit Polyclonal Antibody to Adenosine A2B Receptor (extracellular) conjugated to the fluorescent dye Allophycocyanin (APC)
    • Host: Rabbit
    • Label: APC
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: ADORA2b, A2BAR, Adenosine A2b Receptor
  • Anti-Adenosine A<sub>2B</sub> Receptor (extracellular)-FITC Antibody
    Anti-Adenosine A2B Receptor (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to Adenosine A2B Receptor Conjugated to the Fluorescent Dye FITC
    • Product Code: AAR-003-F
    • Short Description: A Rabbit Polyclonal Antibody to Adenosine A2B Receptor Conjugated to the Fluorescent Dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Human, Rat
    • Applications: Flow cytometry
    • Target: ADORA2b, A2BAR, Adenosine A2bR
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