Thrombosis and Atherosclerosis

Thrombosis and Atherosclerosis

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  • The expected molecular weight (MW) listed in the product datasheet is based solely on the size of the target protein’s amino acid sequence. It’s important to remember that many factors can affect the banding pattern of your western blot including: (1) the existence of a splice variant, (2) the quality of the loaded sample, (3) the protein extraction method, and (4) the protein transfer conditions. To achieve accurate results, you may need to systematically adjust the protein extraction method or the protein transfer conditions.

    • If the band’s MW is below the expected MW it could be due to a splice variant with a slightly different MW.
      • Heat the samples at 70°C for 10 min.
      • Increase the transfer time.
    • If the band’s MW is above the expected MW, it could be due to post-translational modifications.
    • In either event, you should use a blocking peptide as a negative control.

  • Some toxins and peptides are soluble in DMSO; you can find this information in the product specification section. For these products, prepare a concentrated stock solution first.

    1. Centrifuge vial (10,000 x g, 5 minutes) before adding solvent.
    2. Dissolve the lyophilized reagent in DMSO. We recommend keeping a concentrated stock solution at 1-10 mM (100-1000X higher than the final working concentration).
    3. Once the peptide is completely dissolved in DMSO, slowly dilute the peptide in pure water (or buffer) to the desired final working concentration.

    Note: We recommend that you maintain a DMSO concentration as low as possible. For cell assays, a final concentration of 0.1–0.5% DMSO (v/v) is considered acceptable. For other experiments, 5% DMSO (v/v) is recommended; adjust this according to your experimental requirements.

  • FAQ 3FAQ 3FAQ 3FAQ 3

239 Results
  • Anti-Angiotensin II Receptor Type-2 (extracellular) Antibody
    KO Validate'
    Anti-Angiotensin II Receptor Type-2 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Angiotensin II Receptor Type-2
    • Product Code: AAR-012
    • Short Description: A Rabbit Polyclonal Antibody to Angiotensin II Receptor Type-2
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: AT2 Receptor, AT2R, AGTR2, MRX88
  • Anti-Angiotensin II Receptor Type-2 (extracellular)-ATTO Fluor-488 Antibody
    KO Validate'
    Anti-Angiotensin II Receptor Type-2 (extracellular)-ATTO Fluor-488 Antibody
    A Rabbit Polyclonal Antibody to Angiotensin II Receptor Type-2 Conjugated to the Fluorescent Dye ATTO-488
    • Product Code: AAR-012-AG
    • Short Description: A Rabbit Polyclonal Antibody to Angiotensin II Receptor Type-2 Conjugated to the Fluorescent Dye ATTO-488
    • Host: Rabbit
    • Label: ATTO 488
    • Reactivity: Rat, Mouse
    • Applications: Live cell imaging, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: AT2 Receptor, AT2R, AGTR2, MRX88
  • Anti-ASIC4 Antibody
    Anti-ASIC4 Antibody
    A Rabbit Polyclonal Antibody to ASIC4 Channel
    • Product Code: ASC-015
    • Short Description: A Rabbit Polyclonal Antibody to ASIC4 Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot
    • Target: Acid-sensing ion channel 4, H+-gated cation channel, Amiloride-sensitive cation channel 4, BNaC4, SPASIC, ACCN4
  • Anti-CD63 (extracellular) Antibody
    Anti-CD63 (extracellular) Antibody
    A Rabbit Polyclonal antibody to CD63 (extracellular)
    • Product Code: ANR-210
    • Short Description: A Rabbit Polyclonal antibody to CD63 (extracellular)
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunohistochemistry
    • Target: Tetraspanin-30, TSPAN30, Lysosomal-Associated Membrane Protein 3, LAMP-3, Granulophysin, Melanoma-Associated Antigen ME491
  • Anti-CD63 (extracellular)-APC Antibody
    Anti-CD63 (extracellular)-APC Antibody
    A Rabbit Polyclonal antibody to CD63 (extracellular) conjugated to the fluorescent dye Allophycocyanin (APC)
    • Product Code: ANR-210-APC
    • Short Description: A Rabbit Polyclonal antibody to CD63 (extracellular) conjugated to the fluorescent dye Allophycocyanin (APC)
    • Host: Rabbit
    • Label: APC
    • Reactivity: Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: Tetraspanin-30, TSPAN30, Lysosomal-Associated Membrane Protein 3, LAMP-3, Granulophysin, Melanoma-Associated Antigen ME491
  • Anti-CD63 (extracellular)-FITC Antibody
    Anti-CD63 (extracellular)-FITC Antibody
    A Rabbit Polyclonal antibody to CD63 (extracellular) conjugated to the fluorescent dye FITC
    • Product Code: ANR-210-F
    • Short Description: A Rabbit Polyclonal antibody to CD63 (extracellular) conjugated to the fluorescent dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: Tetraspanin-30, TSPAN30, Lysosomal-Associated Membrane Protein 3, LAMP-3, Granulophysin, Melanoma-Associated Antigen ME491
  • Anti-CD63 (extracellular)-PE Antibody
    Anti-CD63 (extracellular)-PE Antibody
    A Rabbit Polyclonal antibody to CD63 (extracellular) conjugated to the fluorescent dye Phycoerythrin (PE).
    • Product Code: ANR-210-PE
    • Short Description: A Rabbit Polyclonal antibody to CD63 (extracellular) conjugated to the fluorescent dye Phycoerythrin (PE).
    • Host: Rabbit
    • Label: R-PE
    • Reactivity: Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: Tetraspanin-30, TSPAN30, Lysosomal-Associated Membrane Protein 3, LAMP-3, Granulophysin, Melanoma-Associated Antigen ME491
  • Anti-CD73 (extracellular) Antibody
    Anti-CD73 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to CD73
    • Product Code: ANT-066
    • Short Description: A Rabbit Polyclonal Antibody to CD73
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunofluorescence, Immunohistochemistry
    • Target: Ecto-5-nucleotidase, E5NT, 5'-Nucleotidase, 5'-NT, NT5E
  • Anti-CD73 (extracellular)-FITC Antibody
    Anti-CD73 (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to CD73 (extracellular) conjugated to the fluorescent dye FITC
    • Product Code: ANT-066-F
    • Short Description: A Rabbit Polyclonal Antibody to CD73 (extracellular) conjugated to the fluorescent dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Human, Rat, Mouse
    • Applications: Flow cytometry
    • Target: Ecto-5-nucleotidase, E5NT, 5'-Nucleotidase, 5'-NT, NT5E, ENT
  • Anti-CD9 (extracellular) Antibody
    Anti-CD9 (extracellular) Antibody
    A Rabbit Polyclonal antibody to CD9 (extracellular)
    • Product Code: ANR-209
    • Short Description: A Rabbit Polyclonal antibody to CD9 (extracellular)
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunohistochemistry
    • Target: Tetrapanin-29, TSPAN29, Leukocyte Antigen MIC3, MIC3
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