Inflammation

Inflammation

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  • The expected molecular weight (MW) listed in the product datasheet is based solely on the size of the target protein’s amino acid sequence. It’s important to remember that many factors can affect the banding pattern of your western blot including: (1) the existence of a splice variant, (2) the quality of the loaded sample, (3) the protein extraction method, and (4) the protein transfer conditions. To achieve accurate results, you may need to systematically adjust the protein extraction method or the protein transfer conditions.

    • If the band’s MW is below the expected MW it could be due to a splice variant with a slightly different MW.
      • Heat the samples at 70°C for 10 min.
      • Increase the transfer time.
    • If the band’s MW is above the expected MW, it could be due to post-translational modifications.
    • In either event, you should use a blocking peptide as a negative control.
  • Some toxins and peptides are soluble in DMSO; you can find this information in the product specification section. For these products, prepare a concentrated stock solution first.

    1. Centrifuge vial (10,000 x g, 5 minutes) before adding solvent.
    2. Dissolve the lyophilized reagent in DMSO. We recommend keeping a concentrated stock solution at 1-10 mM (100-1000X higher than the final working concentration).
    3. Once the peptide is completely dissolved in DMSO, slowly dilute the peptide in pure water (or buffer) to the desired final working concentration.

    Note: We recommend that you maintain a DMSO concentration as low as possible. For cell assays, a final concentration of 0.1–0.5% DMSO (v/v) is considered acceptable. For other experiments, 5% DMSO (v/v) is recommended; adjust this according to your experimental requirements.

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2041 Results
  • Anti-PACAP Receptor 1 (PAC1) Antibody
    A Rabbit Polyclonal Antibody to PACAP Type I Receptor
    • Product Code: AVR-003
    • Short Description: A Rabbit Polyclonal Antibody to PACAP Type I Receptor
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: PACAP type I receptor, Pituitary adenylate cyclase-activating polypeptide type I receptor, PACAP-R1, ADCYAP1R1
  • Anti-VPAC2 (VIPR2) (extracellular) Antibody
    A Rabbit Polyclonal Antibody to VPAC2 Receptor
    • Product Code: AVR-002
    • Short Description: A Rabbit Polyclonal Antibody to VPAC2 Receptor
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunocytochemistry, Immunofluorescence
    • Target: VIP and PACAP receptor 2, Vasoactive intestinal polypeptide receptor 2, Helodermin-preferring VIP receptor, Pituitary adenylate cyclase-activating polypeptide type III receptor, PACAP type III receptor, PACAP-R3
  • Anti-VPAC1 (VIPR1) (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to VPAC1 Receptor Conjugated to the Fluorescent Dye FITC
    • Product Code: AVR-001-F
    • Short Description: A Rabbit Polyclonal Antibody to VPAC1 Receptor Conjugated to the Fluorescent Dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: VIP and PACAP receptor 1, Vasoactive intestinal polypeptide receptor 1, Pituitary adenylate cyclase-activating polypeptide type II receptor, PACAP type II receptor, PACAP-R2
  • Anti-VPAC1 (VIPR1) (extracellular) Antibody
    A Rabbit Polyclonal Antibody to VPAC1 Receptor
    • Product Code: AVR-001
    • Short Description: A Rabbit Polyclonal Antibody to VPAC1 Receptor
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: VIP and PACAP receptor 1, Vasoactive intestinal polypeptide receptor 1, Pituitary adenylate cyclase-activating polypeptide type II receptor, PACAP type II receptor, PACAP-R2
  • Anti-MERTK (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to MERTK (extracellular) conjugated to the fluorescent dye FITC
    • Product Code: ATR-033-F
    • Short Description: A Rabbit Polyclonal Antibody to MERTK (extracellular) conjugated to the fluorescent dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: Receptor tyrosine kinase MerTK, C-Mer Proto-Oncogene Tyrosine Kinase, Mer, Proto-Oncogene C-Mer
  • Anti-MERTK (extracellular) Antibody
    A Rabbit Polyclonal Antibody to MERTK Receptor
    • Product Code: ATR-033
    • Short Description: A Rabbit Polyclonal Antibody to MERTK Receptor
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Western blot, Immunofluorescence, Immunohistochemistry
    • Target: Receptor tyrosine kinase MerTK, C-Mer Proto-Oncogene Tyrosine Kinase, Mer, Proto-Oncogene C-Mer
  • Anti-AXL (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to AXL (extracellular) conjugated to the fluorescent dye FITC
    • Product Code: ATR-032-F
    • Short Description: A Rabbit Polyclonal Antibody to AXL (extracellular) conjugated to the fluorescent dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: AXL Receptor Tyrosine Kinase, Tyrosine-Protein Kinase Receptor UFO, AXL Oncogene, Tyro7, UFO, ARK
  • Anti-AXL (extracellular) Antibody
    A Rabbit Polyclonal antibody to AXL (extracellular)
    • Product Code: ATR-032
    • Short Description: A Rabbit Polyclonal antibody to AXL (extracellular)
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunohistochemistry
    • Target: AXL Receptor Tyrosine Kinase, Tyrosine-Protein Kinase Receptor UFO, AXL Oncogene, Tyro7, UFO
  • Anti-TYRO3 (extracellular) Antibody
    A Rabbit Polyclonal antibody to TYRO3 (extracellular)
    • Product Code: ATR-031
    • Short Description: A Rabbit Polyclonal antibody to TYRO3 (extracellular)
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot
    • Target: Tyrosine-Protein Kinase Receptor TYRO3, Tyrosine-Protein Kinase DTK, Tyrosine-Protein Kinase RSE, Tyrosine-Protein Kinase SKY
  • Anti-Trace Amine Receptor 1 (TAAR1) Antibody
    A Rabbit Polyclonal Antibody to Trace Amine Receptor 1
    • Product Code: ATR-021
    • Short Description: A Rabbit Polyclonal Antibody to Trace Amine Receptor 1
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot
    • Target: Trace amine-associated receptor 1, TaR-1, TA1, TRAR1
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