Inflammation

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  • The expected molecular weight (MW) listed in the product datasheet is based solely on the size of the target protein’s amino acid sequence. It’s important to remember that many factors can affect the banding pattern of your western blot including: (1) the existence of a splice variant, (2) the quality of the loaded sample, (3) the protein extraction method, and (4) the protein transfer conditions. To achieve accurate results, you may need to systematically adjust the protein extraction method or the protein transfer conditions.

    • If the band’s MW is below the expected MW it could be due to a splice variant with a slightly different MW.
      • Heat the samples at 70°C for 10 min.
      • Increase the transfer time.
    • If the band’s MW is above the expected MW, it could be due to post-translational modifications.
    • In either event, you should use a blocking peptide as a negative control.

  • Some toxins and peptides are soluble in DMSO; you can find this information in the product specification section. For these products, prepare a concentrated stock solution first.

    1. Centrifuge vial (10,000 x g, 5 minutes) before adding solvent.
    2. Dissolve the lyophilized reagent in DMSO. We recommend keeping a concentrated stock solution at 1-10 mM (100-1000X higher than the final working concentration).
    3. Once the peptide is completely dissolved in DMSO, slowly dilute the peptide in pure water (or buffer) to the desired final working concentration.

    Note: We recommend that you maintain a DMSO concentration as low as possible. For cell assays, a final concentration of 0.1–0.5% DMSO (v/v) is considered acceptable. For other experiments, 5% DMSO (v/v) is recommended; adjust this according to your experimental requirements.

  • FAQ 3FAQ 3FAQ 3FAQ 3

2041 Results
  • Anti-Taste Receptor Type 1 Member 3 (TAS1R3) (extracellular) Antibody
    Anti-Taste Receptor Type 1 Member 3 (TAS1R3) (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Taste Receptor Type 1 Member 3
    • Product Code: ATR-016
    • Short Description: A Rabbit Polyclonal Antibody to Taste Receptor Type 1 Member 3
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunohistochemistry
    • Target: Sweet Taste Receptor T1R3, T1R3
  • Anti-TSH Receptor (TSHR) (extracellular)-ATTO Fluor-488 Antibody
    Anti-TSH Receptor (TSHR) (extracellular)-ATTO Fluor-488 Antibody
    A Rabbit Polyclonal Antibody to TSH Receptor Conjugated to the Fluorescent Dye ATTO-488
    • Product Code: ATR-006-AG
    • Short Description: A Rabbit Polyclonal Antibody to TSH Receptor Conjugated to the Fluorescent Dye ATTO-488
    • Host: Rabbit
    • Label: ATTO 488
    • Reactivity: Human, Rat, Mouse
    • Applications: Immunofluorescence, Immunohistochemistry
    • Target: Thyrotropin receptor, Thyroid-stimulating hormone receptor, TSH-R
  • Anti-TSH Receptor (TSHR) (extracellular) Antibody
    Anti-TSH Receptor (TSHR) (extracellular) Antibody
    A Rabbit Polyclonal Antibody to TSH Receptor
    • Product Code: ATR-006
    • Short Description: A Rabbit Polyclonal Antibody to TSH Receptor
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunohistochemistry
    • Target: Thyrotropin receptor, Thyroid-stimulating hormone receptor, TSH-R
  • Anti-TRHR1 Antibody
    Anti-TRHR1 Antibody
    A Rabbit Polyclonal Antibody to Thyrotropin-Releasing Hormone Receptor 1
    • Product Code: ATR-005
    • Short Description: A Rabbit Polyclonal Antibody to Thyrotropin-Releasing Hormone Receptor 1
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunocytochemistry, Immunofluorescence
    • Target: Thyrotropin-releasing hormone receptor 1, TRH1 receptor, TRHR, Thyroliberin receptor
  • Anti-Neurokinin 1 Receptor (NK1R) (extracellular)-FITC Antibody
    Anti-Neurokinin 1 Receptor (NK1R) (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to Neurokinin 1 Receptor Conjugated to the Fluorescent Dye FITC
    • Product Code: ATR-001-F
    • Short Description: A Rabbit Polyclonal Antibody to Neurokinin 1 Receptor Conjugated to the Fluorescent Dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: NK1 receptor, Tachykinin receptor 1, TACR1, Substance-P receptor, SPR
  • Anti-Neurokinin 1 Receptor (NK1R) (extracellular)-APC Antibody
    Anti-Neurokinin 1 Receptor (NK1R) (extracellular)-APC Antibody
    A Rabbit Polyclonal Antibody to Neurokinin 1 Receptor Conjugated to the Fluorescent Dye Allophycocyanin (APC).
    • Product Code: ATR-001-APC
    • Short Description: A Rabbit Polyclonal Antibody to Neurokinin 1 Receptor Conjugated to the Fluorescent Dye Allophycocyanin (APC).
    • Host: Rabbit
    • Label: APC
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: NK1 receptor, Tachykinin receptor 1, TACR1, Substance-P receptor, SPR
  • Anti-Neurokinin 1 Receptor (NK1R) (extracellular)-ATTO Fluor-488 Antibody
    KO Validate'
    Anti-Neurokinin 1 Receptor (NK1R) (extracellular)-ATTO Fluor-488 Antibody
    A Rabbit Polyclonal Antibody to Neurokinin 1 Receptor Conjugated to the Fluorescent Dye ATTO-488
    • Product Code: ATR-001-AG
    • Short Description: A Rabbit Polyclonal Antibody to Neurokinin 1 Receptor Conjugated to the Fluorescent Dye ATTO-488
    • Host: Rabbit
    • Label: ATTO 488
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry, Immunohistochemistry
    • Target: NK1 receptor, Tachykinin receptor 1, TACR1, Substance-P receptor, SPR
  • Anti-Neurokinin 1 Receptor (NK1R) (extracellular) Antibody
    Anti-Neurokinin 1 Receptor (NK1R) (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Neurokinin 1 Receptor
    • Product Code: ATR-001
    • Short Description: A Rabbit Polyclonal Antibody to Neurokinin 1 Receptor
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunocytochemistry, Immunohistochemistry
    • Target: NK1 receptor, Tachykinin receptor 1, TACR1, Substance-P receptor, SPR
  • Anti-TMC6 (EVER1) Antibody
    Anti-TMC6 (EVER1) Antibody
    A Rabbit Polyclonal Antibody to TMC6 Channel
    • Product Code: ATC-006
    • Short Description: A Rabbit Polyclonal Antibody to TMC6 Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Mouse
    • Applications: Western blot
    • Target: Transmembrane channel-like protein 6, Epidermodysplasia verruciformis protein 1, Protein LAK-4, LAK4P, EVIN1, EV1
  • Anti-GPR91 (SUCNR1) (extracellular)-PE Antibody
    Anti-GPR91 (SUCNR1) (extracellular)-PE Antibody
    A Rabbit Polyclonal Antibody to GPR91 (SUCNR1) (extracellular) conjugated to the fluorescent dye Phycoerythrin (PE)
    • Product Code: ASR-090-PE
    • Short Description: A Rabbit Polyclonal Antibody to GPR91 (SUCNR1) (extracellular) conjugated to the fluorescent dye Phycoerythrin (PE)
    • Host: Rabbit
    • Label: R-PE
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: G-protein coupled receptor 91, Succinate receptor 1
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