Inflammation

Inflammation

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  • The expected molecular weight (MW) listed in the product datasheet is based solely on the size of the target protein’s amino acid sequence. It’s important to remember that many factors can affect the banding pattern of your western blot including: (1) the existence of a splice variant, (2) the quality of the loaded sample, (3) the protein extraction method, and (4) the protein transfer conditions. To achieve accurate results, you may need to systematically adjust the protein extraction method or the protein transfer conditions.

    • If the band’s MW is below the expected MW it could be due to a splice variant with a slightly different MW.
      • Heat the samples at 70°C for 10 min.
      • Increase the transfer time.
    • If the band’s MW is above the expected MW, it could be due to post-translational modifications.
    • In either event, you should use a blocking peptide as a negative control.
  • Some toxins and peptides are soluble in DMSO; you can find this information in the product specification section. For these products, prepare a concentrated stock solution first.

    1. Centrifuge vial (10,000 x g, 5 minutes) before adding solvent.
    2. Dissolve the lyophilized reagent in DMSO. We recommend keeping a concentrated stock solution at 1-10 mM (100-1000X higher than the final working concentration).
    3. Once the peptide is completely dissolved in DMSO, slowly dilute the peptide in pure water (or buffer) to the desired final working concentration.

    Note: We recommend that you maintain a DMSO concentration as low as possible. For cell assays, a final concentration of 0.1–0.5% DMSO (v/v) is considered acceptable. For other experiments, 5% DMSO (v/v) is recommended; adjust this according to your experimental requirements.

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2041 Results
  • Anti-Aquaporin 1 Antibody
    A Rabbit Polyclonal Antibody to Aquaporin 1 Channel
    • Product Code: AQP-001
    • Short Description: A Rabbit Polyclonal Antibody to Aquaporin 1 Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: AQP1, Aquaporin-CHIP, CHIP28, Urine water channel, Water channel protein for red blood cells and kidney proximal tubule
  • Anti-IQGAP1 Antibody
    A Rabbit Polyclonal Antibody to IQGAP1
    • Product Code: APZ-055
    • Short Description: A Rabbit Polyclonal Antibody to IQGAP1
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: IQ Motif Containing GTPase Activating Protein 1, Ras GTPase-Activating-Like Protein IQGAP1, p195
  • Anti-MAGI2/AIP1 Antibody
    A Rabbit Polyclonal Antibody to MAGI2
    • Product Code: APZ-052
    • Short Description: A Rabbit Polyclonal Antibody to MAGI2
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot
    • Target: Membrane associated guanylate kinase, WW and PDZ domain containing 2, Activin receptor-interacting protein 1, Acvrip1, Atrophin-1-interacting protein 1
  • Anti-DLGAP1/GKAP Antibody
    A Rabbit Polyclonal Antibody to DLGAP1/GKAP/SAPAP1
    • Product Code: APZ-041
    • Short Description: A Rabbit Polyclonal Antibody to DLGAP1/GKAP/SAPAP1
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: DLG associated protein 1, Discs large homolog associated protein 1, Guanylate kinase-associated protein, SAP90/PSD-95-associated protein 1, SAPAP1
  • Anti-Gamma-Synuclein Antibody
    A Rabbit Polyclonal Antibody to Gamma-Synuclein
    • Product Code: APZ-037
    • Short Description: A Rabbit Polyclonal Antibody to Gamma-Synuclein
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: γ-Synuclein, Breast Cancer-Specific Gene 1 Protein, BCSG1, Persyn, Synoretin, SNCG
  • Guinea Pig Anti-Alpha-Synuclein Antibody
    A Guinea Pig Polyclonal Antibody to α-Synuclein
    • Product Code: APZ-035-GP
    • Short Description: A Guinea Pig Polyclonal Antibody to α-Synuclein
    • Host: Guinea Pig
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Western blot, Immunohistochemistry
    • Target: α-Synuclein, Non-A4 component of amyloid precursor, Parkinson disease (autosomal dominant, Lewy body) 4, PARK1, PARK4, NACP, SNCA
  • Anti-Alpha-Synuclein Antibody
    A Rabbit Polyclonal Antibody to α-Synuclein
    • Product Code: APZ-035
    • Short Description: A Rabbit Polyclonal Antibody to α-Synuclein
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Western blot
    • Target: α-Synuclein, Non-A4 component of amyloid precursor, Parkinson disease (autosomal dominant, Lewy body) 4, PARK1, PARK4, NACP, SNCA
  • Anti-AKAP12 Antibody
    A Rabbit Polyclonal Antibody to AKAP12
    • Product Code: APZ-033
    • Short Description: A Rabbit Polyclonal Antibody to AKAP12
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: A-kinase anchoring protein 12, A-kinase anchor protein 250 kDa, AKAP250, Gravin, Myasthenia gravis autoantigen gravin, Src-suppressed C kinase substrate, SSeCKS
  • Anti-Homer3 Antibody
    A Rabbit Polyclonal Antibody to Homer3
    • Product Code: APZ-028
    • Short Description: A Rabbit Polyclonal Antibody to Homer3
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: Homer protein homolog 3, Homer scaffold protein 3, VESL3, Homer-3
  • Anti-Homer2 Antibody
    A Rabbit Polyclonal Antibody to Homer2
    • Product Code: APZ-027
    • Short Description: A Rabbit Polyclonal Antibody to Homer2
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: Homer protein homolog 2, Cupidin, Vesl-2
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