Inflammation

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  • The expected molecular weight (MW) listed in the product datasheet is based solely on the size of the target protein’s amino acid sequence. It’s important to remember that many factors can affect the banding pattern of your western blot including: (1) the existence of a splice variant, (2) the quality of the loaded sample, (3) the protein extraction method, and (4) the protein transfer conditions. To achieve accurate results, you may need to systematically adjust the protein extraction method or the protein transfer conditions.

    • If the band’s MW is below the expected MW it could be due to a splice variant with a slightly different MW.
      • Heat the samples at 70°C for 10 min.
      • Increase the transfer time.
    • If the band’s MW is above the expected MW, it could be due to post-translational modifications.
    • In either event, you should use a blocking peptide as a negative control.

  • Some toxins and peptides are soluble in DMSO; you can find this information in the product specification section. For these products, prepare a concentrated stock solution first.

    1. Centrifuge vial (10,000 x g, 5 minutes) before adding solvent.
    2. Dissolve the lyophilized reagent in DMSO. We recommend keeping a concentrated stock solution at 1-10 mM (100-1000X higher than the final working concentration).
    3. Once the peptide is completely dissolved in DMSO, slowly dilute the peptide in pure water (or buffer) to the desired final working concentration.

    Note: We recommend that you maintain a DMSO concentration as low as possible. For cell assays, a final concentration of 0.1–0.5% DMSO (v/v) is considered acceptable. For other experiments, 5% DMSO (v/v) is recommended; adjust this according to your experimental requirements.

  • FAQ 3FAQ 3FAQ 3FAQ 3

2041 Results
  • Anti-P2Y13 Receptor (extracellular) Antibody
    Anti-P2Y13 Receptor (extracellular) Antibody
    A Rabbit Polyclonal antibody to P2Y13 (extracellular).
    • Product Code: APR-103
    • Short Description: A Rabbit Polyclonal antibody to P2Y13 (extracellular).
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot
    • Target: Purinergic Receptor P2Y13, P2RY13, GPCR1, GPR86, GPR94
  • Anti-Thromboxane A2 Receptor (extracellular)-FITC Antibody
    Anti-Thromboxane A2 Receptor (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to Thromboxane A2 Receptor (extracellular) conjugated to the fluorescent dye FITC
    • Product Code: APR-095-F
    • Short Description: A Rabbit Polyclonal Antibody to Thromboxane A2 Receptor (extracellular) conjugated to the fluorescent dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: Prostanoid TP receptor, TXA2-R, TBXA2R
  • Anti-Thromboxane A2 Receptor (extracellular) Antibody
    Anti-Thromboxane A2 Receptor (extracellular) Antibody
    A Rabbit Polyclonal antibody to Thromboxane A2 Receptor (extracellular).
    • Product Code: APR-095
    • Short Description: A Rabbit Polyclonal antibody to Thromboxane A2 Receptor (extracellular).
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot
    • Target: Prostanoid TP receptor, TXA2-R, TBXA2R
  • Anti-Thromboxane A2 Receptor Antibody
    Anti-Thromboxane A2 Receptor Antibody
    A Rabbit Polyclonal Antibody to Thromboxane A2 Receptor
    • Product Code: APR-069
    • Short Description: A Rabbit Polyclonal Antibody to Thromboxane A2 Receptor
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: Prostanoid TP receptor, TXA2-R, TBXA2R
  • Anti-Prostacyclin Receptor (PTGIR) (extracellular)-FITC Antibody
    Anti-Prostacyclin Receptor (PTGIR) (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to PTGIR (extracellular) conjugated to the fluorescent dye FITC
    • Product Code: APR-068-F
    • Short Description: A Rabbit Polyclonal Antibody to PTGIR (extracellular) conjugated to the fluorescent dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: Prostanoid IP receptor, Prostaglandin I2 receptor, PGI2 receptor, PRIPR
  • Anti-Prostacyclin Receptor (PTGIR) (extracellular) Antibody
    Anti-Prostacyclin Receptor (PTGIR) (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Prostacyclin Receptor (PTGIR)
    • Product Code: APR-068
    • Short Description: A Rabbit Polyclonal Antibody to Prostacyclin Receptor (PTGIR)
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot
    • Target: Prostanoid IP receptor, Prostaglandin I2 receptor, PGI2 receptor
  • Anti-Prostaglandin F2-α Receptor/PTGFR Antibody
    Anti-Prostaglandin F2-α Receptor/PTGFR Antibody
    A Rabbit Polyclonal Antibody to Prostaglandin F2-α Receptor
    • Product Code: APR-067
    • Short Description: A Rabbit Polyclonal Antibody to Prostaglandin F2-α Receptor
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: Prostaglandin F2-alpha receptor, Prostaglandin F receptor, Prostanoid FP receptor, PGF2-alpha receptor
  • Anti-Prostaglandin E Receptor EP4 (PTGER4) Antibody
    Anti-Prostaglandin E Receptor EP4 (PTGER4) Antibody
    A Rabbit Polyclonal Antibody to Prostanoid EP4 Receptor
    • Product Code: APR-066
    • Short Description: A Rabbit Polyclonal Antibody to Prostanoid EP4 Receptor
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Western blot
    • Target: Prostaglandin E2 receptor EP4 subtype, PGE2 receptor EP4 subtype, Prostanoid EP4 receptor
  • Anti-Prostaglandin E Receptor EP3 (PTGER3) Antibody
    Anti-Prostaglandin E Receptor EP3 (PTGER3) Antibody
    A Rabbit Polyclonal antibody to the Prostanoid EP3 Receptor
    • Product Code: APR-065
    • Short Description: A Rabbit Polyclonal antibody to the Prostanoid EP3 Receptor
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Western blot, Immunohistochemistry
    • Target: Prostaglandin E2 receptor EP3 subtype, PGE2 receptor EP3 subtype, Prostanoid EP3 receptor
  • Anti-Prostaglandin E Receptor EP2/PTGER2 (extracellular)-PE Antibody
    Anti-Prostaglandin E Receptor EP2/PTGER2 (extracellular)-PE Antibody
    A Rabbit polyclonal antibody to Prostaglandin E Receptor EP2/PTGER2 (extracellular) conjugated to R-Phycoerythrin (R-PE) fluorophore.
    • Product Code: APR-064-PE
    • Short Description: A Rabbit polyclonal antibody to Prostaglandin E Receptor EP2/PTGER2 (extracellular) conjugated to R-Phycoerythrin (R-PE) fluorophore.
    • Host: Rabbit
    • Label: R-PE
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: Prostaglandin E2 receptor EP2 subtype, PGE2 receptor EP2 subtype, Prostanoid EP2 receptor
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