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  • The expected molecular weight (MW) listed in the product datasheet is based solely on the size of the target protein’s amino acid sequence. It’s important to remember that many factors can affect the banding pattern of your western blot including: (1) the existence of a splice variant, (2) the quality of the loaded sample, (3) the protein extraction method, and (4) the protein transfer conditions. To achieve accurate results, you may need to systematically adjust the protein extraction method or the protein transfer conditions.

    • If the band’s MW is below the expected MW it could be due to a splice variant with a slightly different MW.
      • Heat the samples at 70°C for 10 min.
      • Increase the transfer time.
    • If the band’s MW is above the expected MW, it could be due to post-translational modifications.
    • In either event, you should use a blocking peptide as a negative control.

  • Some toxins and peptides are soluble in DMSO; you can find this information in the product specification section. For these products, prepare a concentrated stock solution first.

    1. Centrifuge vial (10,000 x g, 5 minutes) before adding solvent.
    2. Dissolve the lyophilized reagent in DMSO. We recommend keeping a concentrated stock solution at 1-10 mM (100-1000X higher than the final working concentration).
    3. Once the peptide is completely dissolved in DMSO, slowly dilute the peptide in pure water (or buffer) to the desired final working concentration.

    Note: We recommend that you maintain a DMSO concentration as low as possible. For cell assays, a final concentration of 0.1–0.5% DMSO (v/v) is considered acceptable. For other experiments, 5% DMSO (v/v) is recommended; adjust this according to your experimental requirements.

  • FAQ 3FAQ 3FAQ 3FAQ 3

2041 Results
  • Anti-Prostaglandin E Receptor EP2/PTGER2 (extracellular)-FITC Antibody
    Anti-Prostaglandin E Receptor EP2/PTGER2 (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to Prostanoid EP2 Receptor Conjugated to the Fluorescent Dye FITC
    • Product Code: APR-064-F
    • Short Description: A Rabbit Polyclonal Antibody to Prostanoid EP2 Receptor Conjugated to the Fluorescent Dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: Prostaglandin E2 receptor EP2 subtype, PGE2 receptor EP2 subtype, Prostanoid EP2 receptor
  • Anti-Prostaglandin E Receptor EP2/PTGER2 (extracellular) Antibody
    Anti-Prostaglandin E Receptor EP2/PTGER2 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Prostanoid EP2 Receptor
    • Product Code: APR-064
    • Short Description: A Rabbit Polyclonal Antibody to Prostanoid EP2 Receptor
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunofluorescence, Immunohistochemistry
    • Target: Prostaglandin E2 receptor EP2 subtype, PGE2 receptor EP2 subtype, Prostanoid EP2 receptor
  • Anti-CRTH2/GPR44 (extracellular) Antibody
    Anti-CRTH2/GPR44 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Prostanoid DP2 Receptor (CRTH2, GPR44)
    • Product Code: APR-062
    • Short Description: A Rabbit Polyclonal Antibody to Prostanoid DP2 Receptor (CRTH2, GPR44)
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunofluorescence, Immunohistochemistry
    • Target: Prostanoid DP2 receptor, Prostaglandin D2 receptor 2, PTGDR2, CD294
  • Anti-Platelet-activating factor receptor (extracellular)-FITC Antibody
    Anti-Platelet-activating factor receptor (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to Platelet-activating factor receptor (extracellular) conjugated to the fluorescent dye FITC
    • Product Code: APR-055-F
    • Short Description: A Rabbit Polyclonal Antibody to Platelet-activating factor receptor (extracellular) conjugated to the fluorescent dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: PTAFR, PAFR
  • Anti-Platelet-activating factor receptor (extracellular) Antibody
    Anti-Platelet-activating factor receptor (extracellular) Antibody
    A Rabbit Polyclonal antibody to Platelet-activating factor receptor (extracellular)
    • Product Code: APR-055
    • Short Description: A Rabbit Polyclonal antibody to Platelet-activating factor receptor (extracellular)
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunohistochemistry
    • Target: PTAFR, PAFR
  • Anti-PTH2R (extracellular)-ATTO Fluor-488 Antibody
    Anti-PTH2R (extracellular)-ATTO Fluor-488 Antibody
    A Rabbit Polyclonal Antibody to Parathyroid Hormone 2 Receptor Conjugated to the Fluorescent Dye ATTO-488
    • Product Code: APR-052-AG
    • Short Description: A Rabbit Polyclonal Antibody to Parathyroid Hormone 2 Receptor Conjugated to the Fluorescent Dye ATTO-488
    • Host: Rabbit
    • Label: ATTO 488
    • Reactivity: Rat, Mouse
    • Applications: Immunofluorescence, Immunohistochemistry
    • Target: Parathyroid hormone 2 receptor, PTH2 receptor, PTHR2
  • Anti-PTH2R (extracellular) Antibody
    Anti-PTH2R (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Parathyroid Hormone 2 Receptor
    • Product Code: APR-052
    • Short Description: A Rabbit Polyclonal Antibody to Parathyroid Hormone 2 Receptor
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Western blot
    • Target: Parathyroid hormone 2 receptor, PTH2 receptor, PTHR2
  • Anti-PTH1R (extracellular) Antibody
    Anti-PTH1R (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Parathyroid Hormone 1 Receptor
    • Product Code: APR-051
    • Short Description: A Rabbit Polyclonal Antibody to Parathyroid Hormone 1 Receptor
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: Parathyroid hormone 1 receptor, PTH/PTHrP type I receptor, PTH1 receptor, PTHR, PTHR1
  • Anti-PAR1 (F2R) (extracellular)-FITC Antibody
    Anti-PAR1 (F2R) (extracellular)-FITC Antibody
    A Rabbit polyclonal antibody to PAR1 (F2R) (extracellular) conjugated to fluorescein isothiocyanate (FITC) fluorophore.
    • Product Code: APR-036-F
    • Short Description: A Rabbit polyclonal antibody to PAR1 (F2R) (extracellular) conjugated to fluorescein isothiocyanate (FITC) fluorophore.
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: Proteinase-activated receptor 1, Coagulation factor II receptor, CF2R
  • Anti-PAR1 (F2R) (extracellular) Antibody
    Anti-PAR1 (F2R) (extracellular) Antibody
    A Rabbit Polyclonal Antibody to PAR1 (F2R) (extracellular)
    • Product Code: APR-036
    • Short Description: A Rabbit Polyclonal Antibody to PAR1 (F2R) (extracellular)
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunohistochemistry
    • Target: Proteinase-activated receptor 1, Coagulation factor II receptor, CF2R
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