Inflammation

Inflammation

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  • The expected molecular weight (MW) listed in the product datasheet is based solely on the size of the target protein’s amino acid sequence. It’s important to remember that many factors can affect the banding pattern of your western blot including: (1) the existence of a splice variant, (2) the quality of the loaded sample, (3) the protein extraction method, and (4) the protein transfer conditions. To achieve accurate results, you may need to systematically adjust the protein extraction method or the protein transfer conditions.

    • If the band’s MW is below the expected MW it could be due to a splice variant with a slightly different MW.
      • Heat the samples at 70°C for 10 min.
      • Increase the transfer time.
    • If the band’s MW is above the expected MW, it could be due to post-translational modifications.
    • In either event, you should use a blocking peptide as a negative control.
  • Some toxins and peptides are soluble in DMSO; you can find this information in the product specification section. For these products, prepare a concentrated stock solution first.

    1. Centrifuge vial (10,000 x g, 5 minutes) before adding solvent.
    2. Dissolve the lyophilized reagent in DMSO. We recommend keeping a concentrated stock solution at 1-10 mM (100-1000X higher than the final working concentration).
    3. Once the peptide is completely dissolved in DMSO, slowly dilute the peptide in pure water (or buffer) to the desired final working concentration.

    Note: We recommend that you maintain a DMSO concentration as low as possible. For cell assays, a final concentration of 0.1–0.5% DMSO (v/v) is considered acceptable. For other experiments, 5% DMSO (v/v) is recommended; adjust this according to your experimental requirements.

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2041 Results
  • Anti-P2X7 Receptor (extracellular)-ATTO Fluor-633 Antibody
    A Rabbit Polyclonal Antibody to P2X7 Receptor Conjugated to the Fluorescent Dye ATTO-633
    • Product Code: APR-008-FR
    • Short Description: A Rabbit Polyclonal Antibody to P2X7 Receptor Conjugated to the Fluorescent Dye ATTO-633
    • Host: Rabbit
    • Label: ATTO 633
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Immunocytochemistry, Immunofluorescence, Flow cytometry
    • Target: Purinergic receptor P2X7, P2RX7, P2Z, ATP receptor
  • Anti-P2X7 Receptor (extracellular)-ATTO Fluor-488 Antibody
    A Rabbit Polyclonal Antibody to P2X7 Receptor Conjugated to the Fluorescent Dye ATTO-488
    • Product Code: APR-008-AG
    • Short Description: A Rabbit Polyclonal Antibody to P2X7 Receptor Conjugated to the Fluorescent Dye ATTO-488
    • Host: Rabbit
    • Label: ATTO 488
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Immunocytochemistry, Immunofluorescence, Flow cytometry, Immunohistochemistry
    • Target: Purinergic receptor P2X7, P2RX7, P2Z, ATP receptor
  • Anti-P2X7 Receptor-ATTO Fluor-550 Antibody
    A Rabbit Polyclonal Antibody to P2X7 Receptor Conjugated to the Fluorescent Dye ATTO-550
    • Product Code: APR-004-AO
    • Short Description: A Rabbit Polyclonal Antibody to P2X7 Receptor Conjugated to the Fluorescent Dye ATTO-550
    • Host: Rabbit
    • Label: ATTO 550
    • Reactivity: Human, Rat, Mouse
    • Applications: Immunofluorescence, Immunohistochemistry
    • Target: Purinergic receptor P2X7, P2RX7, P2Z, ATP receptor
  • Anti-P2X7 Receptor Antibody
    A Rabbit Polyclonal Antibody to P2X7 Receptor
    • Product Code: APR-004
    • Short Description: A Rabbit Polyclonal Antibody to P2X7 Receptor
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry
    • Target: Purinergic receptor P2X7, P2RX7, P2Z, ATP receptor
  • Guinea pig Anti-P2X4 Receptor Antibody
    A Guinea Pig Polyclonal Antibody to P2X4 Receptor
    • Product Code: APR-002-GP
    • Short Description: A Guinea Pig Polyclonal Antibody to P2X4 Receptor
    • Host: Guinea Pig
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot
    • Target: P2RX4, P2X purinoceptor 4, ATP receptor
  • Anti-P2X4 Receptor Antibody
    A Rabbit Polyclonal Antibody to P2X4 Receptor
    • Product Code: APR-002
    • Short Description: A Rabbit Polyclonal Antibody to P2X4 Receptor
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Western blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry
    • Target: P2RX4, P2X purinoceptor 4, ATP receptor
  • Anti-KCNQ1 (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to KV7.1 (extracellular) conjugated to the fluorescent dye FITC
    • Product Code: APC-168-F
    • Short Description: A Rabbit Polyclonal Antibody to KV7.1 (extracellular) conjugated to the fluorescent dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: KV7.1, Voltage-gated potassium channel QKT subfamily member 1, KvLQT1, KCNA9
  • Anti-KCNQ1 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to KCNQ1 (KV7.1) Channel
    • Product Code: APC-168
    • Short Description: A Rabbit Polyclonal Antibody to KCNQ1 (KV7.1) Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunocytochemistry, Immunofluorescence
    • Target: KV7.1, Voltage-gated potassium channel QKT subfamily member 1, KvLQT1, KCNA9
  • Anti-K<sub>ir</sub>4.1 (KCNJ10) (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Kir4.1 (KCNJ10) Channel
    • Product Code: APC-165
    • Short Description: A Rabbit Polyclonal Antibody to Kir4.1 (KCNJ10) Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: ATP-sensitive inward rectifier potassium channel 10, KAB-2, BIR10, BIRK1, Kir1.2, Potassium channel inwardly rectifying subfamily J member 10
  • Anti-KCNQ4 Antibody
    A Rabbit Polyclonal Antibody to KCNQ4 (KV7.4) Channel
    • Product Code: APC-164
    • Short Description: A Rabbit Polyclonal Antibody to KCNQ4 (KV7.4) Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: KV7.4, Potassium voltage-gated channel subfamily KQT member 4, KQT-like 4, KvLQT4, DFNA2
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