Inflammation

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  • The expected molecular weight (MW) listed in the product datasheet is based solely on the size of the target protein’s amino acid sequence. It’s important to remember that many factors can affect the banding pattern of your western blot including: (1) the existence of a splice variant, (2) the quality of the loaded sample, (3) the protein extraction method, and (4) the protein transfer conditions. To achieve accurate results, you may need to systematically adjust the protein extraction method or the protein transfer conditions.

    • If the band’s MW is below the expected MW it could be due to a splice variant with a slightly different MW.
      • Heat the samples at 70°C for 10 min.
      • Increase the transfer time.
    • If the band’s MW is above the expected MW, it could be due to post-translational modifications.
    • In either event, you should use a blocking peptide as a negative control.

  • Some toxins and peptides are soluble in DMSO; you can find this information in the product specification section. For these products, prepare a concentrated stock solution first.

    1. Centrifuge vial (10,000 x g, 5 minutes) before adding solvent.
    2. Dissolve the lyophilized reagent in DMSO. We recommend keeping a concentrated stock solution at 1-10 mM (100-1000X higher than the final working concentration).
    3. Once the peptide is completely dissolved in DMSO, slowly dilute the peptide in pure water (or buffer) to the desired final working concentration.

    Note: We recommend that you maintain a DMSO concentration as low as possible. For cell assays, a final concentration of 0.1–0.5% DMSO (v/v) is considered acceptable. For other experiments, 5% DMSO (v/v) is recommended; adjust this according to your experimental requirements.

  • FAQ 3FAQ 3FAQ 3FAQ 3

2041 Results
  • Anti-Piezo1 (extracellular)-APC Antibody
    Anti-Piezo1 (extracellular)-APC Antibody
    A Rabbit Polyclonal Antibody to Piezo1 (extracellular) conjugated to the fluorescent dye Allophycocyanin (APC)
    • Product Code: APC-087-APC
    • Short Description: A Rabbit Polyclonal Antibody to Piezo1 (extracellular) conjugated to the fluorescent dye Allophycocyanin (APC)
    • Host: Rabbit
    • Label: APC
    • Reactivity: Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: Piezo-type mechanosensitive ion channel component 1, Protein FAM38A, Mib, Membrane protein induced by beta-amyloid treatment
  • Anti-Piezo1 (extracellular) Antibody
    KO Validate'
    Anti-Piezo1 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Piezo1 Channel
    • Product Code: APC-087
    • Short Description: A Rabbit Polyclonal Antibody to Piezo1 Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunofluorescence, Immunohistochemistry
    • Target: Piezo-type mechanosensitive ion channel component 1, Protein FAM38A, Mib, Membrane protein induced by beta-amyloid treatment
  • Anti-LRRC26 Antibody
    Anti-LRRC26 Antibody
    A Rabbit Polyclonal Antibody to LRRC26
    • Product Code: APC-070
    • Short Description: A Rabbit Polyclonal Antibody to LRRC26
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Western blot
    • Target: Leucine-rich repeat-containing protein 26, BK channel auxiliary gamma subunit LRRC26, Cytokeratin-associated protein in cancer, CAPC
  • Anti-KCNN4 (K<sub>Ca</sub>3.1, SK4) Antibody
    Anti-KCNN4 (KCa3.1, SK4) Antibody
    A Rabbit Polyclonal Antibody to KCNN4 (KCa3.1, SK4) Channel
    • Product Code: APC-064
    • Short Description: A Rabbit Polyclonal Antibody to KCNN4 (KCa3.1, SK4) Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry
    • Target: IKCa1, IK1, Intermediate conductance Ca2+-activated K+ channel protein 4, Gardos channel
  • Anti-KCNH2 (HERG) Antibody
    Anti-KCNH2 (HERG) Antibody
    A Rabbit Polyclonal Antibody to KV11.1 Channel
    • Product Code: APC-062
    • Short Description: A Rabbit Polyclonal Antibody to KV11.1 Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry
    • Target: KV11.1, Potassium voltage-gated channel subfamily H member 2, Ether-a-go-go-related channel 1
  • Guinea pig Anti-HCN1 Antibody
    Guinea pig Anti-HCN1 Antibody
    A Guinea Pig Polyclonal Antibody to HCN1 Channel
    • Product Code: APC-056-GP
    • Short Description: A Guinea Pig Polyclonal Antibody to HCN1 Channel
    • Host: Guinea Pig
    • Label: Unconjugated
    • Reactivity: Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: Hyperpolarization-activated cyclic nucleotide-gated potassium channel 1, HAC2, Brain cyclic nucleotide-gated channel 1, BCNG1, Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 1
  • Anti-HCN1 Antibody
    KO Validate'
    Anti-HCN1 Antibody
    A Rabbit Polyclonal Antibody to HCN1 Channel
    • Product Code: APC-056
    • Short Description: A Rabbit Polyclonal Antibody to HCN1 Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: Hyperpolarization-activated cyclic nucleotide-gated potassium channel 1, HAC2, Brain cyclic nucleotide-gated channel 1, BCNG1, Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 1
  • Anti-KCNE2 (MiRP1) Antibody
    Anti-KCNE2 (MiRP1) Antibody
    A Rabbit Polyclonal Antibody to KCNE2 (MiRP1) Subunit
    • Product Code: APC-054
    • Short Description: A Rabbit Polyclonal Antibody to KCNE2 (MiRP1) Subunit
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry
    • Target: Potassium channel subunit β MiRP1, MinK-related peptide 1, Potassium voltage-gated channel subfamily E member 2
  • Guinea pig Anti-HCN4 Antibody
    Guinea pig Anti-HCN4 Antibody
    A Guinea Pig Polyclonal Antibody to HCN4 Channel
    • Product Code: APC-052-GP
    • Short Description: A Guinea Pig Polyclonal Antibody to HCN4 Channel
    • Host: Guinea Pig
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: Hyperpolarization-activated cyclic nucleotide-gated potassium channel 4
  • Anti-HCN4 Antibody
    KO Validate'
    Anti-HCN4 Antibody
    A Rabbit Polyclonal Antibody to HCN4 Channel
    • Product Code: APC-052
    • Short Description: A Rabbit Polyclonal Antibody to HCN4 Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry
    • Target: Hyperpolarization-activated cyclic nucleotide-gated potassium channel 4
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