Inflammation

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  • The expected molecular weight (MW) listed in the product datasheet is based solely on the size of the target protein’s amino acid sequence. It’s important to remember that many factors can affect the banding pattern of your western blot including: (1) the existence of a splice variant, (2) the quality of the loaded sample, (3) the protein extraction method, and (4) the protein transfer conditions. To achieve accurate results, you may need to systematically adjust the protein extraction method or the protein transfer conditions.

    • If the band’s MW is below the expected MW it could be due to a splice variant with a slightly different MW.
      • Heat the samples at 70°C for 10 min.
      • Increase the transfer time.
    • If the band’s MW is above the expected MW, it could be due to post-translational modifications.
    • In either event, you should use a blocking peptide as a negative control.

  • Some toxins and peptides are soluble in DMSO; you can find this information in the product specification section. For these products, prepare a concentrated stock solution first.

    1. Centrifuge vial (10,000 x g, 5 minutes) before adding solvent.
    2. Dissolve the lyophilized reagent in DMSO. We recommend keeping a concentrated stock solution at 1-10 mM (100-1000X higher than the final working concentration).
    3. Once the peptide is completely dissolved in DMSO, slowly dilute the peptide in pure water (or buffer) to the desired final working concentration.

    Note: We recommend that you maintain a DMSO concentration as low as possible. For cell assays, a final concentration of 0.1–0.5% DMSO (v/v) is considered acceptable. For other experiments, 5% DMSO (v/v) is recommended; adjust this according to your experimental requirements.

  • FAQ 3FAQ 3FAQ 3FAQ 3

2045 Results
  • Anti-HCN1 Antibody
    KO Validate'
    Anti-HCN1 Antibody
    A Rabbit Polyclonal Antibody to HCN1 Channel
    • Product Code: APC-056
    • Short Description: A Rabbit Polyclonal Antibody to HCN1 Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: Hyperpolarization-activated cyclic nucleotide-gated potassium channel 1, HAC2, Brain cyclic nucleotide-gated channel 1, BCNG1, Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 1
  • Anti-KCNE2 (MiRP1) Antibody
    Anti-KCNE2 (MiRP1) Antibody
    A Rabbit Polyclonal Antibody to KCNE2 (MiRP1) Subunit
    • Product Code: APC-054
    • Short Description: A Rabbit Polyclonal Antibody to KCNE2 (MiRP1) Subunit
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry
    • Target: Potassium channel subunit β MiRP1, MinK-related peptide 1, Potassium voltage-gated channel subfamily E member 2
  • Guinea pig Anti-HCN4 Antibody
    Guinea pig Anti-HCN4 Antibody
    A Guinea Pig Polyclonal Antibody to HCN4 Channel
    • Product Code: APC-052-GP
    • Short Description: A Guinea Pig Polyclonal Antibody to HCN4 Channel
    • Host: Guinea Pig
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: Hyperpolarization-activated cyclic nucleotide-gated potassium channel 4
  • Anti-HCN4 Antibody
    KO Validate'
    Anti-HCN4 Antibody
    A Rabbit Polyclonal Antibody to HCN4 Channel
    • Product Code: APC-052
    • Short Description: A Rabbit Polyclonal Antibody to HCN4 Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry
    • Target: Hyperpolarization-activated cyclic nucleotide-gated potassium channel 4
  • Anti-KCNQ3 Antibody
    KO Validate'
    Anti-KCNQ3 Antibody
    A Rabbit Polyclonal Antibody to KCNQ3 (KV7.3) Channel
    • Product Code: APC-051
    • Short Description: A Rabbit Polyclonal Antibody to KCNQ3 (KV7.3) Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Western blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry
    • Target: KV7.3, Potassium voltage-gated channel subfamily KQT member 3, BFNC2
  • Guinea pig Anti-KCNQ2 Antibody
    Guinea pig Anti-KCNQ2 Antibody
    A Guinea Pig Polyclonal Antibody to KCNQ2 (KV7.2) Channel
    • Product Code: APC-050-GP
    • Short Description: A Guinea Pig Polyclonal Antibody to KCNQ2 (KV7.2) Channel
    • Host: Guinea Pig
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: KV7.2, Voltage-gated potassium channel QKT subfamily member 2, KvLQT2
  • Anti-KCNQ2 Antibody
    Anti-KCNQ2 Antibody
    A Rabbit Polyclonal Antibody to KCNQ2 (KV7.2) Channel
    • Product Code: APC-050
    • Short Description: A Rabbit Polyclonal Antibody to KCNQ2 (KV7.2) Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: KV7.2, Voltage-gated potassium channel QKT subfamily member 2, KvLQT2
  • Guinea pig Anti-K<sub>ir</sub>4.1 (KCNJ10) Antibody
    KO Validate'
    Guinea pig Anti-Kir4.1 (KCNJ10) Antibody
    A Guinea Pig Polyclonal Antibody to Kir4.1 (KCNJ10) Channel
    • Product Code: APC-035-GP
    • Short Description: A Guinea Pig Polyclonal Antibody to Kir4.1 (KCNJ10) Channel
    • Host: Guinea Pig
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: ATP-sensitive inward rectifier potassium channel 10, KAB-2, BIR10, BIRK1, Kir1.2, Potassium channel inwardly rectifying subfamily J member 10
  • Anti-K<sub>ir</sub>4.1 (KCNJ10)-ATTO Fluor-488 Antibody
    KO Validate'
    Anti-Kir4.1 (KCNJ10)-ATTO Fluor-488 Antibody
    A Rabbit Polyclonal Antibody to Kir4.1 (KCNJ10) Channel Conjugated to the Fluorescent Dye ATTO-488
    • Product Code: APC-035-AG
    • Short Description: A Rabbit Polyclonal Antibody to Kir4.1 (KCNJ10) Channel Conjugated to the Fluorescent Dye ATTO-488
    • Host: Rabbit
    • Label: ATTO 488
    • Reactivity: Human, Rat, Mouse
    • Applications: Immunofluorescence, Immunohistochemistry
    • Target: ATP-sensitive inward rectifier potassium channel 10, KAB-2, BIR10, BIRK1, Kir1.2, Potassium channel inwardly rectifying subfamily J member 10
  • Anti-K<sub>ir</sub>4.1 (KCNJ10) Antibody
    KO Validate'
    Anti-Kir4.1 (KCNJ10) Antibody
    A Rabbit Polyclonal Antibody to Kir4.1 (KCNJ10) Channel
    • Product Code: APC-035
    • Short Description: A Rabbit Polyclonal Antibody to Kir4.1 (KCNJ10) Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry
    • Target: ATP-sensitive inward rectifier potassium channel 10, KAB-2, BIR10, BIRK1, Kir1.2, Potassium channel inwardly rectifying subfamily J member 10
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