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  • The expected molecular weight (MW) listed in the product datasheet is based solely on the size of the target protein’s amino acid sequence. It’s important to remember that many factors can affect the banding pattern of your western blot including: (1) the existence of a splice variant, (2) the quality of the loaded sample, (3) the protein extraction method, and (4) the protein transfer conditions. To achieve accurate results, you may need to systematically adjust the protein extraction method or the protein transfer conditions.

    • If the band’s MW is below the expected MW it could be due to a splice variant with a slightly different MW.
      • Heat the samples at 70°C for 10 min.
      • Increase the transfer time.
    • If the band’s MW is above the expected MW, it could be due to post-translational modifications.
    • In either event, you should use a blocking peptide as a negative control.

  • Some toxins and peptides are soluble in DMSO; you can find this information in the product specification section. For these products, prepare a concentrated stock solution first.

    1. Centrifuge vial (10,000 x g, 5 minutes) before adding solvent.
    2. Dissolve the lyophilized reagent in DMSO. We recommend keeping a concentrated stock solution at 1-10 mM (100-1000X higher than the final working concentration).
    3. Once the peptide is completely dissolved in DMSO, slowly dilute the peptide in pure water (or buffer) to the desired final working concentration.

    Note: We recommend that you maintain a DMSO concentration as low as possible. For cell assays, a final concentration of 0.1–0.5% DMSO (v/v) is considered acceptable. For other experiments, 5% DMSO (v/v) is recommended; adjust this according to your experimental requirements.

  • FAQ 3FAQ 3FAQ 3FAQ 3

2041 Results
  • Guinea pig Anti-K<sub>ir</sub>2.1/KCNJ2 Antibody
    Guinea pig Anti-Kir2.1/KCNJ2 Antibody
    A Guinea Pig Polyclonal Antibody to Kir2.1 (KCNJ2) Channel
    • Product Code: APC-026-GP
    • Short Description: A Guinea Pig Polyclonal Antibody to Kir2.1 (KCNJ2) Channel
    • Host: Guinea Pig
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot
    • Target: Inward rectifier potassium channel 2, IRK1, HIRK1, LQT7, SQT3
  • Anti-K<sub>ir</sub>2.1/KCNJ2 Antibody
    Anti-Kir2.1/KCNJ2 Antibody
    A Rabbit Polyclonal Antibody to Kir2.1 (KCNJ2) Channel
    • Product Code: APC-026
    • Short Description: A Rabbit Polyclonal Antibody to Kir2.1 (KCNJ2) Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Western blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry
    • Target: Inward rectifier potassium channel 2, IRK1, HIRK1, LQT7, SQT3
  • Anti-KCNN3 (K<sub>Ca</sub>2.3, SK3) (N-term)-ATTO Fluor-594 Antibody
    KO Validate'
    Anti-KCNN3 (KCa2.3, SK3) (N-term)-ATTO Fluor-594 Antibody
    A Rabbit Polyclonal Antibody to KCNN3 (KCa2.3, SK3) Channel Conjugated to the Fluorescent Dye ATTO-594
    • Product Code: APC-025-AR
    • Short Description: A Rabbit Polyclonal Antibody to KCNN3 (KCa2.3, SK3) Channel Conjugated to the Fluorescent Dye ATTO-594
    • Host: Rabbit
    • Label: ATTO 594
    • Reactivity: Human, Rat, Mouse
    • Applications: Immunocytochemistry, Immunofluorescence, Flow cytometry, Immunohistochemistry
    • Target: Small conductance calcium-activated potassium channel protein 3, SKCa3
  • Anti-KCNN3 (K<sub>Ca</sub>2.3, SK3) (N-term) Antibody
    KO Validate'
    Anti-KCNN3 (KCa2.3, SK3) (N-term) Antibody
    A Rabbit Polyclonal Antibody to KCNN3 (KCa2.3, SK3) Channel
    • Product Code: APC-025
    • Short Description: A Rabbit Polyclonal Antibody to KCNN3 (KCa2.3, SK3) Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: Small conductance calcium-activated potassium channel protein 3, SKCa3
  • Anti-KCNK3 (TASK-1) Antibody
    Anti-KCNK3 (TASK-1) Antibody
    A Rabbit Polyclonal Antibody to KCNK3 Channel
    • Product Code: APC-024
    • Short Description: A Rabbit Polyclonal Antibody to KCNK3 Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Mouse
    • Applications: Western blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry
    • Target: Potassium channel subfamily K member 3, TWIK-related acid sensitive K+ channel, K2P3.1, cTBAK-1, OAT1
  • Guinea pig Anti-K<sub>V</sub>4.2 Antibody
    Guinea pig Anti-KV4.2 Antibody
    A Guinea Pig Polyclonal Antibody to KV4.2 Channel
    • Product Code: APC-023-GP
    • Short Description: A Guinea Pig Polyclonal Antibody to KV4.2 Channel
    • Host: Guinea Pig
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: Voltage-gated potassium channel subfamily D member 2, KCND2, Shal1, RK5
  • Anti-K<sub>V</sub>4.2-ATTO Fluor-633 Antibody
    Anti-KV4.2-ATTO Fluor-633 Antibody
    A Rabbit Polyclonal Antibody to KV4.2 Channel Conjugated to the Fluorescent Dye ATTO-633
    • Product Code: APC-023-FR
    • Short Description: A Rabbit Polyclonal Antibody to KV4.2 Channel Conjugated to the Fluorescent Dye ATTO-633
    • Host: Rabbit
    • Label: ATTO 633
    • Reactivity: Rat, Mouse
    • Applications: Immunofluorescence, Immunohistochemistry
    • Target: Voltage-gated potassium channel subfamily D member 2, KCND2, Shal1, RK5
  • Anti-K<sub>V</sub>4.2 Antibody
    Anti-KV4.2 Antibody
    A Rabbit Polyclonal Antibody to KV4.2 Channel
    • Product Code: APC-023
    • Short Description: A Rabbit Polyclonal Antibody to KV4.2 Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry
    • Target: Voltage-gated potassium channel subfamily D member 2, KCND2, Shal1, RK5
  • Guinea pig Anti-KCNQ1 Antibody
    Guinea pig Anti-KCNQ1 Antibody
    A Guinea Pig Polyclonal Antibody to KCNQ1 (KV7.1) Channel
    • Product Code: APC-022-GP
    • Short Description: A Guinea Pig Polyclonal Antibody to KCNQ1 (KV7.1) Channel
    • Host: Guinea Pig
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunohistochemistry
    • Target: KV7.1, Voltage-gated potassium channel QKT subfamily member 1, KVLQT1
  • Anti-KCNQ1 Antibody
    Anti-KCNQ1 Antibody
    A Rabbit Polyclonal Antibody to KCNQ1 (KV7.1) Channel
    • Product Code: APC-022
    • Short Description: A Rabbit Polyclonal Antibody to KCNQ1 (KV7.1) Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry
    • Target: KV7.1, Voltage-gated potassium channel QKT subfamily member 1, KvLQT1
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