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  • The expected molecular weight (MW) listed in the product datasheet is based solely on the size of the target protein’s amino acid sequence. It’s important to remember that many factors can affect the banding pattern of your western blot including: (1) the existence of a splice variant, (2) the quality of the loaded sample, (3) the protein extraction method, and (4) the protein transfer conditions. To achieve accurate results, you may need to systematically adjust the protein extraction method or the protein transfer conditions.

    • If the band’s MW is below the expected MW it could be due to a splice variant with a slightly different MW.
      • Heat the samples at 70°C for 10 min.
      • Increase the transfer time.
    • If the band’s MW is above the expected MW, it could be due to post-translational modifications.
    • In either event, you should use a blocking peptide as a negative control.

  • Some toxins and peptides are soluble in DMSO; you can find this information in the product specification section. For these products, prepare a concentrated stock solution first.

    1. Centrifuge vial (10,000 x g, 5 minutes) before adding solvent.
    2. Dissolve the lyophilized reagent in DMSO. We recommend keeping a concentrated stock solution at 1-10 mM (100-1000X higher than the final working concentration).
    3. Once the peptide is completely dissolved in DMSO, slowly dilute the peptide in pure water (or buffer) to the desired final working concentration.

    Note: We recommend that you maintain a DMSO concentration as low as possible. For cell assays, a final concentration of 0.1–0.5% DMSO (v/v) is considered acceptable. For other experiments, 5% DMSO (v/v) is recommended; adjust this according to your experimental requirements.

  • FAQ 3FAQ 3FAQ 3FAQ 3

2041 Results
  • Guinea pig Anti-KCNMA1 (K<sub>Ca</sub>1.1) (1097-1196) Antibody
    Guinea pig Anti-KCNMA1 (KCa1.1) (1097-1196) Antibody
    A Guinea Pig Polyclonal Antibody to KCNMA1 (KCa1.1) Channel
    • Product Code: APC-021-GP
    • Short Description: A Guinea Pig Polyclonal Antibody to KCNMA1 (KCa1.1) Channel
    • Host: Guinea Pig
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot
    • Target: Large conductance calcium-activated potassium channel subfamily M subunit alpha-1, BKCa alpha, Maxi K+, Slo1
  • Anti-KCNMA1 (K<sub>Ca</sub>1.1) (1097-1196) Antibody
    KO Validate'
    Anti-KCNMA1 (KCa1.1) (1097-1196) Antibody
    A Rabbit Polyclonal Antibody to KCNMA1 (KCa1.1) Channel
    • Product Code: APC-021
    • Short Description: A Rabbit Polyclonal Antibody to KCNMA1 (KCa1.1) Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Western blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry
    • Target: Large conductance calcium-activated potassium channel subfamily M subunit alpha-1, BKCa alpha, Maxi K+, Slo1
  • Guinea pig Anti-K<sub>ir</sub>6.2 Antibody
    Guinea pig Anti-Kir6.2 Antibody
    A Guinea Pig Polyclonal Antibody to Kir6.2 Channel
    • Product Code: APC-020-GP
    • Short Description: A Guinea Pig Polyclonal Antibody to Kir6.2 Channel
    • Host: Guinea Pig
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: Inward rectifier K+ channel Kir6.2, ATP-sensitive inward rectifier potassium channel 11, KCNJ11, BIR, TNDM3
  • Anti-K<sub>ir</sub>6.2 Antibody
    KO Validate'
    Anti-Kir6.2 Antibody
    A Rabbit Polyclonal Antibody to Kir6.2 Channel
    • Product Code: APC-020
    • Short Description: A Rabbit Polyclonal Antibody to Kir6.2 Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Western blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry
    • Target: Inward rectifier K+ channel Kir6.2, ATP-sensitive inward rectifier potassium channel 11, KCNJ11, BIR, TNDM3
  • Anti-K<sub>V</sub>4.3 Antibody
    Anti-KV4.3 Antibody
    A Rabbit Polyclonal Antibody to KV4.3 Channel
    • Product Code: APC-017
    • Short Description: A Rabbit Polyclonal Antibody to KV4.3 Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry
    • Target: Voltage-gated K+ channel subfamily D member 3, KCND3
  • Anti-KCNH2 (erg1) Antibody
    KO Validate'
    Anti-KCNH2 (erg1) Antibody
    A Rabbit Polyclonal Antibody to KV11.1 (ERG-1) Channel
    • Product Code: APC-016
    • Short Description: A Rabbit Polyclonal Antibody to KV11.1 (ERG-1) Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry
    • Target: KV11.1, Potassium voltage-gated channel subfamily H member 2, Ether-a-go-go-related channel 1, Eag-related protein 1, ERG-1, HERG
  • Guinea pig Anti-K<sub>V</sub>2.1 Antibody
    Guinea pig Anti-KV2.1 Antibody
    A Guinea Pig Polyclonal Antibody to KV2.1 Channel
    • Product Code: APC-012-GP
    • Short Description: A Guinea Pig Polyclonal Antibody to KV2.1 Channel
    • Host: Guinea Pig
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: KCNB1, DRK1, Delayed rectifier potassium channel 1, Potassium voltage-gated channel subfamily B member 1
  • Anti-K<sub>V</sub>2.1 Antibody
    Anti-KV2.1 Antibody
    A Rabbit Polyclonal Antibody to KV2.1 Channel
    • Product Code: APC-012
    • Short Description: A Rabbit Polyclonal Antibody to KV2.1 Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: KCNB1, DRK1, Delayed rectifier potassium channel 1, Potassium voltage-gated channel subfamily B member 1
  • Anti-K<sub>V</sub>1.1 (KCNA1) Antibody
    Anti-KV1.1 (KCNA1) Antibody
    A Rabbit Polyclonal Antibody to KV1.1 (KCNA1) Channel
    • Product Code: APC-009
    • Short Description: A Rabbit Polyclonal Antibody to KV1.1 (KCNA1) Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry
    • Target: Potassium voltage-gated channel subfamily A member 1
  • Guinea pig Anti-GIRK2 (K<sub>ir</sub>3.2) Antibody
    Guinea pig Anti-GIRK2 (Kir3.2) Antibody
    A Guinea Pig Polyclonal Antibody to GIRK2 (Kir3.2) Channel
    • Product Code: APC-006-GP
    • Short Description: A Guinea Pig Polyclonal Antibody to GIRK2 (Kir3.2) Channel
    • Host: Guinea Pig
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: G protein-activated inward rectifier potassium channel 2, Kcnj6
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