Inflammation

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  • The expected molecular weight (MW) listed in the product datasheet is based solely on the size of the target protein’s amino acid sequence. It’s important to remember that many factors can affect the banding pattern of your western blot including: (1) the existence of a splice variant, (2) the quality of the loaded sample, (3) the protein extraction method, and (4) the protein transfer conditions. To achieve accurate results, you may need to systematically adjust the protein extraction method or the protein transfer conditions.

    • If the band’s MW is below the expected MW it could be due to a splice variant with a slightly different MW.
      • Heat the samples at 70°C for 10 min.
      • Increase the transfer time.
    • If the band’s MW is above the expected MW, it could be due to post-translational modifications.
    • In either event, you should use a blocking peptide as a negative control.

  • Some toxins and peptides are soluble in DMSO; you can find this information in the product specification section. For these products, prepare a concentrated stock solution first.

    1. Centrifuge vial (10,000 x g, 5 minutes) before adding solvent.
    2. Dissolve the lyophilized reagent in DMSO. We recommend keeping a concentrated stock solution at 1-10 mM (100-1000X higher than the final working concentration).
    3. Once the peptide is completely dissolved in DMSO, slowly dilute the peptide in pure water (or buffer) to the desired final working concentration.

    Note: We recommend that you maintain a DMSO concentration as low as possible. For cell assays, a final concentration of 0.1–0.5% DMSO (v/v) is considered acceptable. For other experiments, 5% DMSO (v/v) is recommended; adjust this according to your experimental requirements.

  • FAQ 3FAQ 3FAQ 3FAQ 3

2041 Results
  • Anti-GIRK2 (K<sub>ir</sub>3.2) Antibody
    KO Validate'
    Anti-GIRK2 (Kir3.2) Antibody
    A Rabbit Polyclonal Antibody to GIRK2 (Kir3.2) Channel
    • Product Code: APC-006
    • Short Description: A Rabbit Polyclonal Antibody to GIRK2 (Kir3.2) Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry
    • Target: G protein-activated inward rectifier potassium channel 2, Kcnj6
  • Anti-K<sub>V</sub>1.5 (KCNA5)-ATTO Fluor-550 Antibody
    Anti-KV1.5 (KCNA5)-ATTO Fluor-550 Antibody
    A Rabbit Polyclonal Antibody to KV1.5 (KCNA5) Channel Conjugated to the Fluorescent Dye ATTO-550
    • Product Code: APC-004-AO
    • Short Description: A Rabbit Polyclonal Antibody to KV1.5 (KCNA5) Channel Conjugated to the Fluorescent Dye ATTO-550
    • Host: Rabbit
    • Label: ATTO 550
    • Reactivity: Human, Rat, Mouse
    • Applications: Immunofluorescence, Immunohistochemistry
    • Target: Potassium voltage-gated channel subfamily A member 5
  • Anti-K<sub>V</sub>1.5 (KCNA5) Antibody
    Anti-KV1.5 (KCNA5) Antibody
    A Rabbit Polyclonal Antibody to KV1.5 (KCNA5) Channel
    • Product Code: APC-004
    • Short Description: A Rabbit Polyclonal Antibody to KV1.5 (KCNA5) Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry
    • Target: Potassium voltage-gated channel subfamily A member 5
  • Anti-OXGR1/GPR99 Antibody
    Anti-OXGR1/GPR99 Antibody
    A Rabbit Polyclonal Antibody to Oxoglutarate Receptor 1
    • Product Code: AOR-016
    • Short Description: A Rabbit Polyclonal Antibody to Oxoglutarate Receptor 1
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: Oxoglutarate receptor 1, 2-Oxoglutarate receptor 1, Alpha-ketoglutarate receptor 1, aKGR, GPR80, P2RY15, P2Y15
  • Anti-δ-Opioid Receptor (OPRD1) (extracellular) Antibody
    Anti-δ-Opioid Receptor (OPRD1) (extracellular) Antibody
    A Rabbit Polyclonal Antibody to ?-Opioid Receptor (OPRD1)
    • Product Code: AOR-014
    • Short Description: A Rabbit Polyclonal Antibody to ?-Opioid Receptor (OPRD1)
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: DOR-1
  • Anti-κ-Opioid Receptor (OPRK1) (extracellular)-FITC Antibody
    Anti-κ-Opioid Receptor (OPRK1) (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to ?-Opioid Receptor (extracellular) conjugated to the fluorescent dye FITC
    • Product Code: AOR-012-F
    • Short Description: A Rabbit Polyclonal Antibody to ?-Opioid Receptor (extracellular) conjugated to the fluorescent dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: Kappa-type opioid receptor, KOR-1, KOR, Opiate receptor kappa 1
  • Anti-κ-Opioid Receptor (OPRK1) (extracellular) Antibody
    Anti-κ-Opioid Receptor (OPRK1) (extracellular) Antibody
    A Rabbit Polyclonal Antibody to ?-Opioid Receptor (OPRK1)
    • Product Code: AOR-012
    • Short Description: A Rabbit Polyclonal Antibody to ?-Opioid Receptor (OPRK1)
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunohistochemistry
    • Target: Kappa-type opioid receptor, KOR-1, Opiate receptor kappa 1
  • Anti-µ-Opioid Receptor/OPRM1 (extracellular)-FITC Antibody
    Anti-µ-Opioid Receptor/OPRM1 (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to OPRM1 Conjugated to the Fluorescent Dye FITC
    • Product Code: AOR-011-F
    • Short Description: A Rabbit Polyclonal Antibody to OPRM1 Conjugated to the Fluorescent Dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: Mu-type opioid receptor, Opioid receptor B, MOR-1, MOP, Mu-opiate receptor
  • Anti-µ-Opioid Receptor (OPRM1) (extracellular)-ATTO Fluor-488 Antibody
    Anti-µ-Opioid Receptor (OPRM1) (extracellular)-ATTO Fluor-488 Antibody
    A Rabbit Polyclonal Antibody to µ-Opioid Receptor (OPRM1) Conjugated to the Fluorescent Dye ATTO-488
    • Product Code: AOR-011-AG
    • Short Description: A Rabbit Polyclonal Antibody to µ-Opioid Receptor (OPRM1) Conjugated to the Fluorescent Dye ATTO-488
    • Host: Rabbit
    • Label: ATTO 488
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: Mu-type opioid receptor, Opioid receptor B, MOR-1, MOP, Mu-opiate receptor
  • Anti-µ-Opioid Receptor (OPRM1) (extracellular) Antibody
    Anti-µ-Opioid Receptor (OPRM1) (extracellular) Antibody
    A Rabbit Polyclonal Antibody to µ-Opioid Receptor (OPRM1)
    • Product Code: AOR-011
    • Short Description: A Rabbit Polyclonal Antibody to µ-Opioid Receptor (OPRM1)
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: Mu-type opioid receptor, Opioid receptor B, MOR-1, MOP, Mu-opiate receptor
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