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  • The expected molecular weight (MW) listed in the product datasheet is based solely on the size of the target protein’s amino acid sequence. It’s important to remember that many factors can affect the banding pattern of your western blot including: (1) the existence of a splice variant, (2) the quality of the loaded sample, (3) the protein extraction method, and (4) the protein transfer conditions. To achieve accurate results, you may need to systematically adjust the protein extraction method or the protein transfer conditions.

    • If the band’s MW is below the expected MW it could be due to a splice variant with a slightly different MW.
      • Heat the samples at 70°C for 10 min.
      • Increase the transfer time.
    • If the band’s MW is above the expected MW, it could be due to post-translational modifications.
    • In either event, you should use a blocking peptide as a negative control.

  • Some toxins and peptides are soluble in DMSO; you can find this information in the product specification section. For these products, prepare a concentrated stock solution first.

    1. Centrifuge vial (10,000 x g, 5 minutes) before adding solvent.
    2. Dissolve the lyophilized reagent in DMSO. We recommend keeping a concentrated stock solution at 1-10 mM (100-1000X higher than the final working concentration).
    3. Once the peptide is completely dissolved in DMSO, slowly dilute the peptide in pure water (or buffer) to the desired final working concentration.

    Note: We recommend that you maintain a DMSO concentration as low as possible. For cell assays, a final concentration of 0.1–0.5% DMSO (v/v) is considered acceptable. For other experiments, 5% DMSO (v/v) is recommended; adjust this according to your experimental requirements.

  • FAQ 3FAQ 3FAQ 3FAQ 3

2041 Results
  • Anti-Orexin Receptor 2 Antibody
    Anti-Orexin Receptor 2 Antibody
    A Rabbit Polyclonal Antibody to Orexin Receptor 2
    • Product Code: AOR-002
    • Short Description: A Rabbit Polyclonal Antibody to Orexin Receptor 2
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: OX2R, Hypocretin receptor type 2, HCRTR2
  • Anti-Na<sup>+</sup>/H<sup>+</sup> Exchanger 3 (NHE-3) Antibody
    Anti-Na+/H+ Exchanger 3 (NHE-3) Antibody
    A Rabbit Polyclonal Antibody to Na+/H+ Exchanger 3 (NHE-3)
    • Product Code: ANX-033
    • Short Description: A Rabbit Polyclonal Antibody to Na+/H+ Exchanger 3 (NHE-3)
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Mouse
    • Applications: Western blot
    • Target: Sodium/hydrogen exchanger 3, SLC9A3
  • Anti-SLC11A1/NRAMP1 (extracellular)-FITC Antibody
    Anti-SLC11A1/NRAMP1 (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to SLC11A1/NRAMP1   (extracellular) conjugated to the fluorescent dye FITC
    • Product Code: ANT-201-F
    • Short Description: A Rabbit Polyclonal Antibody to SLC11A1/NRAMP1   (extracellular) conjugated to the fluorescent dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: Natural Resistance-Associated Macrophage Protein 1, LSH, Solute Carrier Family 11 Member 1
  • Anti-SLC11A1/NRAMP1 (extracellular) Antibody
    Anti-SLC11A1/NRAMP1 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to SLC11A1/NRAMP1
    • Product Code: ANT-201
    • Short Description: A Rabbit Polyclonal Antibody to SLC11A1/NRAMP1
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunohistochemistry
    • Target: Natural Resistance-Associated Macrophage Protein 1, LSH, Solute Carrier Family 11 Member 1
  • Anti-SLC3A2 (CD98) (extracellular) Antibody
    Anti-SLC3A2 (CD98) (extracellular) Antibody
    A Rabbit Polyclonal antibody to CALHM2 (extracellular)
    • Product Code: ANT-192
    • Short Description: A Rabbit Polyclonal antibody to CALHM2 (extracellular)
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunohistochemistry
    • Target: Solute Carrier Family 3 Member 2, 4F2 Cell-Surface Antigen Heavy Chain, 4F2HC, CD98 Antigen Heavy Chain, CD98HC
  • Anti-CAT-1 (SLC7A1) (extracellular) Antibody
    Anti-CAT-1 (SLC7A1) (extracellular) Antibody
    A Rabbit Polyclonal Antibody to CAT-1 (SLC7A1) (extracellular)
    • Product Code: ANT-191
    • Short Description: A Rabbit Polyclonal Antibody to CAT-1 (SLC7A1) (extracellular)
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Western blot, Immunohistochemistry
    • Target: SLC7A1
  • Anti-SLC38A9 Antibody
    Anti-SLC38A9 Antibody
    A Rabbit Polyclonal antibody to SLC38A9
    • Product Code: ANT-189
    • Short Description: A Rabbit Polyclonal antibody to SLC38A9
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunohistochemistry
    • Target: Sodium-Coupled Neutral Amino Acid Transporter 9, Solute Carrier Family 38 Member 9, Up-Regulated In Lung Cancer 11, URLC11
  • Anti-SLC38A2 (SNAT2) Antibody
    Anti-SLC38A2 (SNAT2) Antibody
    A Rabbit Polyclonal antibody to SLC38A2 (SNAT2) Transporter
    • Product Code: ANT-185
    • Short Description: A Rabbit Polyclonal antibody to SLC38A2 (SNAT2) Transporter
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunohistochemistry
    • Target: Solute Carrier Family 38 Member 2, Sodium-Coupled Neutral Amino Acid Transporter 2, System A Amino Acid Transporter 2, System N Amino Acid Transporter 2, SAT2
  • Anti-SLC38A1 Antibody
    Anti-SLC38A1 Antibody
    A Rabbit Polyclonal Antibody to SLC38A1 Transporter
    • Product Code: ANT-184
    • Short Description: A Rabbit Polyclonal Antibody to SLC38A1 Transporter
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunohistochemistry
    • Target: Sodium-coupled neutral amino acid transporter 1, Snat1, Amino acid transporter A1, rATA1, Glutamine transporter, N-system amino acid transporter 2, Ata1, Glnt, Sa2, Sat1
  • Anti-LETM1 Antibody
    Anti-LETM1 Antibody
    A Rabbit Polyclonal Antibody to LETM1
    • Product Code: ANT-183
    • Short Description: A Rabbit Polyclonal Antibody to LETM1
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot
    • Target: Leucine zipper-EF-hand-containing transmembrane protein 1, LETM1 and EF-hand domain-containing protein 1 mitochondrial, Mitochondrial proton/calcium exchanger protein, Mdm38 homolog
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