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  • The expected molecular weight (MW) listed in the product datasheet is based solely on the size of the target protein’s amino acid sequence. It’s important to remember that many factors can affect the banding pattern of your western blot including: (1) the existence of a splice variant, (2) the quality of the loaded sample, (3) the protein extraction method, and (4) the protein transfer conditions. To achieve accurate results, you may need to systematically adjust the protein extraction method or the protein transfer conditions.

    • If the band’s MW is below the expected MW it could be due to a splice variant with a slightly different MW.
      • Heat the samples at 70°C for 10 min.
      • Increase the transfer time.
    • If the band’s MW is above the expected MW, it could be due to post-translational modifications.
    • In either event, you should use a blocking peptide as a negative control.

  • Some toxins and peptides are soluble in DMSO; you can find this information in the product specification section. For these products, prepare a concentrated stock solution first.

    1. Centrifuge vial (10,000 x g, 5 minutes) before adding solvent.
    2. Dissolve the lyophilized reagent in DMSO. We recommend keeping a concentrated stock solution at 1-10 mM (100-1000X higher than the final working concentration).
    3. Once the peptide is completely dissolved in DMSO, slowly dilute the peptide in pure water (or buffer) to the desired final working concentration.

    Note: We recommend that you maintain a DMSO concentration as low as possible. For cell assays, a final concentration of 0.1–0.5% DMSO (v/v) is considered acceptable. For other experiments, 5% DMSO (v/v) is recommended; adjust this according to your experimental requirements.

  • FAQ 3FAQ 3FAQ 3FAQ 3

2045 Results
  • Anti-xCT/SLC7A11 (extracellular)-FITC Antibody
    Anti-xCT/SLC7A11 (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to Cystine/Glutamate Transporter (xCT) Conjugated to the Fluorescent dye FITC
    • Product Code: ANT-111-F
    • Short Description: A Rabbit Polyclonal Antibody to Cystine/Glutamate Transporter (xCT) Conjugated to the Fluorescent dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Human, Rat, Mouse
    • Applications: Flow cytometry
    • Target: Cystine/glutamate transporter, Amino acid transport system xc-, Solute carrier family 7 member 11, Calcium channel blocker resistance protein CCBR1
  • Anti-xCT/SLC7A11 (extracellular) Antibody
    KO Validate'
    Anti-xCT/SLC7A11 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Cystine/Glutamate Transporter (xCT)
    • Product Code: ANT-111
    • Short Description: A Rabbit Polyclonal Antibody to Cystine/Glutamate Transporter (xCT)
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunofluorescence, Immunohistochemistry
    • Target: Cystine/glutamate transporter, Amino acid transport system xc-, Solute carrier family 7 member 11, Calcium channel blocker resistance protein CCBR1
  • Anti-LAT1 (SLC7A5) Antibody
    Anti-LAT1 (SLC7A5) Antibody
    A Rabbit Polyclonal Antibody to LAT1
    • Product Code: ANT-105
    • Short Description: A Rabbit Polyclonal Antibody to LAT1
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Western blot, Immunohistochemistry
    • Target: CD98, Large neutral amino acids transporter small subunit 1, L-type amino acid transporter 1, 4F2 light chain, 4F2LC, Integral membrane protein E16, MPE16, Solute carrier family 7 member 5, TA1, Tumor-associated protein 1
  • Anti-SLC3A1 (extracellular) Antibody
    Anti-SLC3A1 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to SLC3A1
    • Product Code: ANT-100
    • Short Description: A Rabbit Polyclonal Antibody to SLC3A1
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Western blot
    • Target: Neutral and basic amino acid transport protein rBAT, Solute carrier family 3 (cystine, dibasic and neutral amino acid transporters) member 1, B(0,+)-type amino acid transport protein, NBAT, Amino acid transporter 1, ATR1
  • Anti-Human ASCT2/SLC1A5 (extracellular)-PE Antibody
    Anti-Human ASCT2/SLC1A5 (extracellular)-PE Antibody
    A Rabbit Polyclonal Antibody to Human ASCT2/SLC1A5 (extracellular) conjugated to the fluorescent dye R-Phycoerythrin
    • Product Code: ANT-083-PE
    • Short Description: A Rabbit Polyclonal Antibody to Human ASCT2/SLC1A5 (extracellular) conjugated to the fluorescent dye R-Phycoerythrin
    • Host: Rabbit
    • Label: R-PE
    • Reactivity: Human
    • Applications: Live cell imaging, Flow cytometry
    • Target: Alanine/Serine/Cysteine Transporter 2, Neutral amino acid transporter B(0), ATB(0), Sodium-dependent Neutral Amino Acid Transporter Type 2
  • Anti-Human ASCT2/SLC1A5 (extracellular)-FITC Antibody
    KO Validate'
    Anti-Human ASCT2/SLC1A5 (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to SLC1A5 (extracellular) conjugated to the fluorescent dye FITC
    • Product Code: ANT-083-F
    • Short Description: A Rabbit Polyclonal Antibody to SLC1A5 (extracellular) conjugated to the fluorescent dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Human
    • Applications: Live cell imaging, Flow cytometry
    • Target: Alanine/Serine/Cysteine Transporter 2, Neutral amino acid transporter B(0), ATB(0), Sodium-dependent Neutral Amino Acid Transporter Type 2
  • Anti-Human ASCT2/SLC1A5 (extracellular) Antibody
    Anti-Human ASCT2/SLC1A5 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to ASCT2 Transporter
    • Product Code: ANT-083
    • Short Description: A Rabbit Polyclonal Antibody to ASCT2 Transporter
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot
    • Target: Alanine/Serine/Cysteine Transporter 2, Neutral amino acid transporter B(0), ATB(0), Sodium-dependent Neutral Amino Acid Transporter Type 2
  • Anti-ASCT2 (SLC1A5) Antibody
    Anti-ASCT2 (SLC1A5) Antibody
    A Rabbit Polyclonal Antibody to ASCT2 Transporter
    • Product Code: ANT-082
    • Short Description: A Rabbit Polyclonal Antibody to ASCT2 Transporter
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: Alanine/serine/cysteine transporter 2, Neutral amino acid transporter B(0), ATB(0), ATBO, Sodium-dependent neutral amino acid transporter type 2, AAAT
  • Anti-ASCT1 (SLC1A4) Antibody
    Anti-ASCT1 (SLC1A4) Antibody
    A Rabbit Polyclonal Antibody to ASCT1 Transporter
    • Product Code: ANT-081
    • Short Description: A Rabbit Polyclonal Antibody to ASCT1 Transporter
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: Alanine/serine/cysteine/threonine transporter 1, Neutral amino acid transporter A, SATT
  • Anti-SLC4A4 (NBC1) (extracellular) Antibody
    Anti-SLC4A4 (NBC1) (extracellular) Antibody
    A Rabbit Polyclonal Antibody to NBC1 Transporter
    • Product Code: ANT-075
    • Short Description: A Rabbit Polyclonal Antibody to NBC1 Transporter
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Live cell imaging, Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: Electrogenic sodium bicarbonate cotransporter 1, NBCe1, NBC-like protein, Na+/HCO3- cotransporter
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