Inflammation

Inflammation

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  • The expected molecular weight (MW) listed in the product datasheet is based solely on the size of the target protein’s amino acid sequence. It’s important to remember that many factors can affect the banding pattern of your western blot including: (1) the existence of a splice variant, (2) the quality of the loaded sample, (3) the protein extraction method, and (4) the protein transfer conditions. To achieve accurate results, you may need to systematically adjust the protein extraction method or the protein transfer conditions.

    • If the band’s MW is below the expected MW it could be due to a splice variant with a slightly different MW.
      • Heat the samples at 70°C for 10 min.
      • Increase the transfer time.
    • If the band’s MW is above the expected MW, it could be due to post-translational modifications.
    • In either event, you should use a blocking peptide as a negative control.
  • Some toxins and peptides are soluble in DMSO; you can find this information in the product specification section. For these products, prepare a concentrated stock solution first.

    1. Centrifuge vial (10,000 x g, 5 minutes) before adding solvent.
    2. Dissolve the lyophilized reagent in DMSO. We recommend keeping a concentrated stock solution at 1-10 mM (100-1000X higher than the final working concentration).
    3. Once the peptide is completely dissolved in DMSO, slowly dilute the peptide in pure water (or buffer) to the desired final working concentration.

    Note: We recommend that you maintain a DMSO concentration as low as possible. For cell assays, a final concentration of 0.1–0.5% DMSO (v/v) is considered acceptable. For other experiments, 5% DMSO (v/v) is recommended; adjust this according to your experimental requirements.

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2041 Results
  • Anti-GFR alpha 1 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to GDNF Family Receptor α1
    • Product Code: ANT-021
    • Short Description: A Rabbit Polyclonal Antibody to GDNF Family Receptor α1
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: GFRA1, GFRα1, GDNF family receptor α1, GDNF receptor alpha 1, Ret ligand 1, RETL1, TRNR1
  • Anti-TrkC (extracellular) Antibody
    A Rabbit Polyclonal Antibody to TrkC
    • Product Code: ANT-020
    • Short Description: A Rabbit Polyclonal Antibody to TrkC
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: Neurotrophic tyrosine kinase receptor 3, NT-3 growth factor receptor, GP145-TrkC, NTRK3
  • Guinea Pig Anti-TrkB (extracellular) Antibody
    A Guinea Pig Polyclonal Antibody to TrkB
    • Product Code: ANT-019-GP
    • Short Description: A Guinea Pig Polyclonal Antibody to TrkB
    • Host: Guinea Pig
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunohistochemistry
    • Target: Neurotrophic tyrosine kinase receptor type 2, NTRK2, BDNF/NT-3 growth factors receptor, TrkB Tyrosine Kinase
  • Anti-TrkB (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to TrkB Conjugated to the fluorescent dye FITC
    • Product Code: ANT-019-F
    • Short Description: A Rabbit Polyclonal Antibody to TrkB Conjugated to the fluorescent dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: Neurotrophic tyrosine kinase receptor type 2, NTRK2, BDNF/NT-3 growth factors receptor
  • Anti-TrkB (extracellular)-ATTO Fluor-488 Antibody
    A Rabbit Polyclonal Antibody to TrkB Conjugated to the Fluorescent Dye ATTO-488
    • Product Code: ANT-019-AG
    • Short Description: A Rabbit Polyclonal Antibody to TrkB Conjugated to the Fluorescent Dye ATTO-488
    • Host: Rabbit
    • Label: ATTO 488
    • Reactivity: Human, Rat, Mouse
    • Applications: Immunofluorescence, Flow cytometry, Immunohistochemistry
    • Target: Neurotrophic tyrosine kinase receptor type 2, NTRK2, BDNF/NT-3 growth factors receptor
  • Anti-TrkA (extracellular)-ATTO Fluor-633 Antibody
    A Rabbit Polyclonal Antibody to TrkA Conjugated to the Fluorescent Dye ATTO-633
    • Product Code: ANT-018-FR
    • Short Description: A Rabbit Polyclonal Antibody to TrkA Conjugated to the Fluorescent Dye ATTO-633
    • Host: Rabbit
    • Label: ATTO 633
    • Reactivity: Human, Rat, Mouse
    • Applications: Immunofluorescence, Immunohistochemistry
    • Target: High affinity nerve growth factor receptor, Neurotrophic tyrosine kinase receptor type 1, NTRK1
  • Anti-TrkA (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to TrkA Conjugated to the Fluorescent Dye FITC
    • Product Code: ANT-018-F
    • Short Description: A Rabbit Polyclonal Antibody to TrkA Conjugated to the Fluorescent Dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Human, Rat, Mouse
    • Applications: Flow cytometry
    • Target: High affinity nerve growth factor receptor, Neurotrophic tyrosine kinase receptor type 1, NTRK1
  • Anti-Neurotensin Receptor 1 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Neurotensin Receptor 1
    • Product Code: ANT-015
    • Short Description: A Rabbit Polyclonal Antibody to Neurotensin Receptor 1
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: NTR1, NTSR1, NTS1, High-affinity levocabastine-insensitive neurotensin receptor, NTRH, NTRR
  • Anti-GDNF Antibody
    A Rabbit Polyclonal Antibody to GDNF
    • Product Code: ANT-014
    • Short Description: A Rabbit Polyclonal Antibody to GDNF
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: Glial-derived neurotrophic factor, hGDNF, Astrocyte-derived trophic factor
  • Anti-proNT-3 Antibody
    A Rabbit Polyclonal Antibody to proNT-3
    • Product Code: ANT-012
    • Short Description: A Rabbit Polyclonal Antibody to proNT-3
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: pro-Neurotrophin 3, proNTF3
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