Inflammation

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  • The expected molecular weight (MW) listed in the product datasheet is based solely on the size of the target protein’s amino acid sequence. It’s important to remember that many factors can affect the banding pattern of your western blot including: (1) the existence of a splice variant, (2) the quality of the loaded sample, (3) the protein extraction method, and (4) the protein transfer conditions. To achieve accurate results, you may need to systematically adjust the protein extraction method or the protein transfer conditions.

    • If the band’s MW is below the expected MW it could be due to a splice variant with a slightly different MW.
      • Heat the samples at 70°C for 10 min.
      • Increase the transfer time.
    • If the band’s MW is above the expected MW, it could be due to post-translational modifications.
    • In either event, you should use a blocking peptide as a negative control.

  • Some toxins and peptides are soluble in DMSO; you can find this information in the product specification section. For these products, prepare a concentrated stock solution first.

    1. Centrifuge vial (10,000 x g, 5 minutes) before adding solvent.
    2. Dissolve the lyophilized reagent in DMSO. We recommend keeping a concentrated stock solution at 1-10 mM (100-1000X higher than the final working concentration).
    3. Once the peptide is completely dissolved in DMSO, slowly dilute the peptide in pure water (or buffer) to the desired final working concentration.

    Note: We recommend that you maintain a DMSO concentration as low as possible. For cell assays, a final concentration of 0.1–0.5% DMSO (v/v) is considered acceptable. For other experiments, 5% DMSO (v/v) is recommended; adjust this according to your experimental requirements.

  • FAQ 3FAQ 3FAQ 3FAQ 3

2041 Results
  • Anti-p75 NGF Receptor (intracellular) Antibody
    Anti-p75 NGF Receptor (intracellular) Antibody
    A Rabbit Polyclonal Antibody to Human Neurotrophin Receptor p75NTR
    • Product Code: ANT-011
    • Short Description: A Rabbit Polyclonal Antibody to Human Neurotrophin Receptor p75NTR
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: p75NTR, p75 Neurotrophin receptor, Low affinity NGF receptor, TNFR superfamily member 16, TNFRSF16, CD271
  • Guinea pig Anti-BDNF Antibody
    Guinea pig Anti-BDNF Antibody
    A Guinea Pig Polyclonal Antibody to BDNF
    • Product Code: ANT-010-GP
    • Short Description: A Guinea Pig Polyclonal Antibody to BDNF
    • Host: Guinea Pig
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: Brain-derived neurotrophic factor
  • Anti-BDNF Antibody
    KO Validate'
    Anti-BDNF Antibody
    A Rabbit Polyclonal Antibody to BDNF
    • Product Code: ANT-010
    • Short Description: A Rabbit Polyclonal Antibody to BDNF
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: Brain-derived neurotrophic factor
  • Anti-Sortilin (extracellular)-FITC Antibody
    Anti-Sortilin (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to Sortilin Conjugated to the Fluorescent Dye FITC
    • Product Code: ANT-009-F
    • Short Description: A Rabbit Polyclonal Antibody to Sortilin Conjugated to the Fluorescent Dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Human, Rat, Mouse
    • Applications: Flow cytometry
    • Target: Sortilin 1, SORT1, Neurotensin receptor 3, NTR3, NT3, Glycoprotein 95, Gp95
  • Anti-Sortilin (extracellular) Antibody
    KO Validate'
    Anti-Sortilin (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Sortilin
    • Product Code: ANT-009
    • Short Description: A Rabbit Polyclonal Antibody to Sortilin
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunofluorescence, Immunohistochemistry
    • Target: SORT1, Neurotensin receptor 3, NTR3, NT3, Glycoprotein 95, Gp95
  • Anti-p75 NGF Receptor (extracellular)-FITC Antibody
    Anti-p75 NGF Receptor (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to Human Neurotrophin Receptor p75NTR Conjugated to the Fluorescent Dye FITC
    • Product Code: ANT-007-F
    • Short Description: A Rabbit Polyclonal Antibody to Human Neurotrophin Receptor p75NTR Conjugated to the Fluorescent Dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: p75NTR, p75 Neurotrophin receptor, Low affinity NGF receptor, TNFR superfamily member 16, TNFRSF16, CD271
  • Anti-p75 NGF Receptor (extracellular)-ATTO Fluor-550 Antibody
    Anti-p75 NGF Receptor (extracellular)-ATTO Fluor-550 Antibody
    A Rabbit Polyclonal Antibody to Human Neurotrophin Receptor p75NTR Conjugated to the Fluorescent Dye ATTO-550
    • Product Code: ANT-007-AO
    • Short Description: A Rabbit Polyclonal Antibody to Human Neurotrophin Receptor p75NTR Conjugated to the Fluorescent Dye ATTO-550
    • Host: Rabbit
    • Label: ATTO 550
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: p75NTR, p75 Neurotrophin receptor, Low affinity NGF receptor, TNFR superfamily member 16, TNFRSF16, CD271
  • Anti-p75 NGF Receptor (extracellular)-ATTO Fluor-488 Antibody
    Anti-p75 NGF Receptor (extracellular)-ATTO Fluor-488 Antibody
    A Rabbit Polyclonal Antibody to Human Neurotrophin Receptor p75NTR Conjugated to the Fluorescent Dye ATTO-488
    • Product Code: ANT-007-AG
    • Short Description: A Rabbit Polyclonal Antibody to Human Neurotrophin Receptor p75NTR Conjugated to the Fluorescent Dye ATTO-488
    • Host: Rabbit
    • Label: ATTO 488
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Immunocytochemistry, Immunofluorescence, Flow cytometry, Immunohistochemistry
    • Target: p75NTR, p75 Neurotrophin receptor, Low affinity NGF receptor, TNFR superfamily member 16, TNFRSF16, CD271
  • Guinea pig Anti-proBDNF Antibody
    Guinea pig Anti-proBDNF Antibody
    A Guinea Pig Polyclonal Antibody to proBDNF
    • Product Code: ANT-006-GP
    • Short Description: A Guinea Pig Polyclonal Antibody to proBDNF
    • Host: Guinea Pig
    • Label: Unconjugated
    • Reactivity: Human, Rat
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: Brain-derived neurotrophic factor precursor
  • Anti-proBDNF-ATTO Fluor-488 Antibody
    Anti-proBDNF-ATTO Fluor-488 Antibody
    A Rabbit Polyclonal Antibody to proBDNF Conjugated to the Fluorescent Dye ATTO-488
    • Product Code: ANT-006-AG
    • Short Description: A Rabbit Polyclonal Antibody to proBDNF Conjugated to the Fluorescent Dye ATTO-488
    • Host: Rabbit
    • Label: ATTO 488
    • Reactivity: Human, Rat, Mouse
    • Applications: Immunofluorescence, Flow cytometry, Immunohistochemistry
    • Target: Brain-derived neurotrophic factor precursor
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