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  • The expected molecular weight (MW) listed in the product datasheet is based solely on the size of the target protein’s amino acid sequence. It’s important to remember that many factors can affect the banding pattern of your western blot including: (1) the existence of a splice variant, (2) the quality of the loaded sample, (3) the protein extraction method, and (4) the protein transfer conditions. To achieve accurate results, you may need to systematically adjust the protein extraction method or the protein transfer conditions.

    • If the band’s MW is below the expected MW it could be due to a splice variant with a slightly different MW.
      • Heat the samples at 70°C for 10 min.
      • Increase the transfer time.
    • If the band’s MW is above the expected MW, it could be due to post-translational modifications.
    • In either event, you should use a blocking peptide as a negative control.

  • Some toxins and peptides are soluble in DMSO; you can find this information in the product specification section. For these products, prepare a concentrated stock solution first.

    1. Centrifuge vial (10,000 x g, 5 minutes) before adding solvent.
    2. Dissolve the lyophilized reagent in DMSO. We recommend keeping a concentrated stock solution at 1-10 mM (100-1000X higher than the final working concentration).
    3. Once the peptide is completely dissolved in DMSO, slowly dilute the peptide in pure water (or buffer) to the desired final working concentration.

    Note: We recommend that you maintain a DMSO concentration as low as possible. For cell assays, a final concentration of 0.1–0.5% DMSO (v/v) is considered acceptable. For other experiments, 5% DMSO (v/v) is recommended; adjust this according to your experimental requirements.

  • FAQ 3FAQ 3FAQ 3FAQ 3

2045 Results
  • Anti-TMEM119 (extracellular)-FITC Antibody
    Anti-TMEM119 (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to TMEM119 (extracellular) conjugated to the fluorescent dye FITC
    • Product Code: ANR-175-F
    • Short Description: A Rabbit Polyclonal Antibody to TMEM119 (extracellular) conjugated to the fluorescent dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Rat, Mouse
    • Applications: Flow cytometry
    • Target: Transmembrane Protein 119, Osteoblast induction factor, OBIF
  • Anti-TMEM119 (extracellular)-APC Antibody
    Anti-TMEM119 (extracellular)-APC Antibody
    A Rabbit Polyclonal Antibody to TMEM119 (extracellular) conjugated to the fluorescent dye Allophycocyanin (APC)
    • Product Code: ANR-175-APC
    • Short Description: A Rabbit Polyclonal Antibody to TMEM119 (extracellular) conjugated to the fluorescent dye Allophycocyanin (APC)
    • Host: Rabbit
    • Label: APC
    • Reactivity: Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: Transmembrane Protein 119, Osteoblast induction factor, OBIF
  • Anti-TMEM119 (extracellular) Antibody
    Anti-TMEM119 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to TMEM119 (extracellular)
    • Product Code: ANR-175
    • Short Description: A Rabbit Polyclonal Antibody to TMEM119 (extracellular)
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Indirect flow cytometry, Western blot
    • Target: Transmembrane Protein 119, Osteoblast induction factor, OBIF
  • Anti-Contactin-2/CNTN2 (extracellular) Antibody
    Anti-Contactin-2/CNTN2 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Contactin-2 (extracellular)
    • Product Code: ANR-172
    • Short Description: A Rabbit Polyclonal Antibody to Contactin-2 (extracellular)
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunohistochemistry
    • Target: Axonal glycoprotein TAG-1, Axonin-1, Transient axonal glycoprotein 1, TAX-1
  • Anti-Contactin-1/CNTN1 (extracellular) Antibody
    Anti-Contactin-1/CNTN1 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Contactin-1 (extracellular)
    • Product Code: ANR-171
    • Short Description: A Rabbit Polyclonal Antibody to Contactin-1 (extracellular)
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: Neural cell surface protein F3, Glycoprotein GP135, CNTN1
  • Anti-ADAM10 (extracellular)-FITC Antibody
    Anti-ADAM10 (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to ADAM10 (extracellular) conjugated to the fluorescent dye FITC
    • Product Code: ANR-156-F
    • Short Description: A Rabbit Polyclonal Antibody to ADAM10 (extracellular) conjugated to the fluorescent dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: Disintegrin And Metalloproteinase Domain-Containing Protein 10, ADAM Metallopeptidase Domain 10, CD156c
  • Anti-ADAM10 (extracellular) Antibody
    Anti-ADAM10 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to ADAM10 (extracellular)
    • Product Code: ANR-156
    • Short Description: A Rabbit Polyclonal Antibody to ADAM10 (extracellular)
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunohistochemistry
    • Target: Disintegrin And Metalloproteinase Domain-Containing Protein 10, ADAM Metallopeptidase Domain 10, CD156c
  • Anti-NMNAT2 Antibody
    Anti-NMNAT2 Antibody
    A Rabbit Polyclonal Antibody to NMNAT2
    • Product Code: ANR-139
    • Short Description: A Rabbit Polyclonal Antibody to NMNAT2
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunohistochemistry
    • Target: Nicotinamide mononucleotide adenylyltransferase 2, Nicotinamide Nucleotide Adenylyltransferase 2, Nicotinamide/Nicotinic Acid Mononucleotide Adenylyltransferase 2, NMN adenylyltransferase 2
  • Anti-SARM1 Antibody
    Anti-SARM1 Antibody
    A Rabbit Polyclonal Antibody to SARM1
    • Product Code: ANR-138
    • Short Description: A Rabbit Polyclonal Antibody to SARM1
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunohistochemistry
    • Target: Sterile Alpha And TIR Motif-Containing Protein 1, NAD(+) Hydrolase SARM1, Sterile Alpha And Armadillo Repeat Protein
  • Anti-SORL1 (extracellular) Antibody
    Anti-SORL1 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to SORL1 Receptor
    • Product Code: ANR-132
    • Short Description: A Rabbit Polyclonal Antibody to SORL1 Receptor
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Western blot, Immunohistochemistry
    • Target: Sortilin Related Receptor 1, Low-Density Lipoprotein Receptor relative with 11 Ligand-Binding Repeats, LR11, Sorting Protein-Related Receptor Containing LDLR Class A Repeats, SORLA
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