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  • The expected molecular weight (MW) listed in the product datasheet is based solely on the size of the target protein’s amino acid sequence. It’s important to remember that many factors can affect the banding pattern of your western blot including: (1) the existence of a splice variant, (2) the quality of the loaded sample, (3) the protein extraction method, and (4) the protein transfer conditions. To achieve accurate results, you may need to systematically adjust the protein extraction method or the protein transfer conditions.

    • If the band’s MW is below the expected MW it could be due to a splice variant with a slightly different MW.
      • Heat the samples at 70°C for 10 min.
      • Increase the transfer time.
    • If the band’s MW is above the expected MW, it could be due to post-translational modifications.
    • In either event, you should use a blocking peptide as a negative control.

  • Some toxins and peptides are soluble in DMSO; you can find this information in the product specification section. For these products, prepare a concentrated stock solution first.

    1. Centrifuge vial (10,000 x g, 5 minutes) before adding solvent.
    2. Dissolve the lyophilized reagent in DMSO. We recommend keeping a concentrated stock solution at 1-10 mM (100-1000X higher than the final working concentration).
    3. Once the peptide is completely dissolved in DMSO, slowly dilute the peptide in pure water (or buffer) to the desired final working concentration.

    Note: We recommend that you maintain a DMSO concentration as low as possible. For cell assays, a final concentration of 0.1–0.5% DMSO (v/v) is considered acceptable. For other experiments, 5% DMSO (v/v) is recommended; adjust this according to your experimental requirements.

  • FAQ 3FAQ 3FAQ 3FAQ 3

2041 Results
  • Anti-NRG1 Type III (extracellular) Antibody
    Anti-NRG1 Type III (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Neuregulin-1 Type III
    • Product Code: ANR-113
    • Short Description: A Rabbit Polyclonal Antibody to Neuregulin-1 Type III
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunohistochemistry
    • Target: Neuregulin-1 isoform 10, CRD-NRG, Sensory and motor neuron derived factor, SMDF
  • Anti-NRG1 (Neuregulin-1) (extracellular)-ATTO Fluor-488 Antibody
    Anti-NRG1 (Neuregulin-1) (extracellular)-ATTO Fluor-488 Antibody
    A Rabbit Polyclonal Antibody to Neuregulin-1 Conjugated to the Fluorescent Dye ATTO-488
    • Product Code: ANR-111-AG
    • Short Description: A Rabbit Polyclonal Antibody to Neuregulin-1 Conjugated to the Fluorescent Dye ATTO-488
    • Host: Rabbit
    • Label: ATTO 488
    • Reactivity: Mouse
    • Applications: Immunofluorescence, Immunohistochemistry
    • Target: Glial growth factor, GGF, Heregulin, HRG, Sensory and motor neuron derived factor, SMDF, Neu differentiation factor, NDF
  • Anti-NRG1 (Neuregulin-1) (extracellular) Antibody
    Anti-NRG1 (Neuregulin-1) (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Neuregulin-1
    • Product Code: ANR-111
    • Short Description: A Rabbit Polyclonal Antibody to Neuregulin-1
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: Glial growth factor, GGF, Heregulin, HRG, Sensory and motor neuron derived factor, SMDF, Neu differentiation factor, NDF
  • Anti-LRRK2 Antibody
    Anti-LRRK2 Antibody
    A Rabbit Polyclonal Antibody to LRRK2
    • Product Code: ANR-102
    • Short Description: A Rabbit Polyclonal Antibody to LRRK2
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: Leucine-rich repeat kinase 2, Parkinson disease (Autosomal dominant) 8, PARK8, Roco2, RIPK7, Dardarin
  • Guinea pig Anti-Periaxin Antibody
    Guinea pig Anti-Periaxin Antibody
    A Guinea Pig Polyclonal Antibody to Periaxin
    • Product Code: ANR-091-GP
    • Short Description: A Guinea Pig Polyclonal Antibody to Periaxin
    • Host: Guinea Pig
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunohistochemistry
    • Target: PRX, CMT4F
  • Anti-Periaxin Antibody
    Anti-Periaxin Antibody
    A Rabbit Polyclonal Antibody to Periaxin
    • Product Code: ANR-091
    • Short Description: A Rabbit Polyclonal Antibody to Periaxin
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: PRX, CMT4F
  • Anti-N-Cadherin-ATTO Fluor-594 Antibody
    Anti-N-Cadherin-ATTO Fluor-594 Antibody
    A Rabbit Polyclonal Antibody to N-Cadherin Conjugated to the Fluorescent Dye ATTO-594
    • Product Code: ANR-082-AR
    • Short Description: A Rabbit Polyclonal Antibody to N-Cadherin Conjugated to the Fluorescent Dye ATTO-594
    • Host: Rabbit
    • Label: ATTO 594
    • Reactivity: Human, Rat, Mouse
    • Applications: Immunofluorescence, Immunohistochemistry
    • Target: Cadherin 2, Neural cadherin, CDH2, NCAD, CDHN, CD325, Calcium-dependent adhesion protein neuronal
  • Anti-N-Cadherin Antibody
    Anti-N-Cadherin Antibody
    A Rabbit Polyclonal Antibody to N-Cadherin
    • Product Code: ANR-082
    • Short Description: A Rabbit Polyclonal Antibody to N-Cadherin
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunohistochemistry
    • Target: Cadherin 2, Neural cadherin, CDH2, NCAD, CDHN, CD325, Calcium-dependent adhesion protein neuronal
  • Anti-Neuropilin-1 (NRP1) (extracellular)-FITC Antibody
    Anti-Neuropilin-1 (NRP1) (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to Neuropilin-1 (NRP1) (extracellular) conjugated to the fluorescent dye FITC.
    • Product Code: ANR-063-F
    • Short Description: A Rabbit Polyclonal Antibody to Neuropilin-1 (NRP1) (extracellular) conjugated to the fluorescent dye FITC.
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Human, Rat, Mouse
    • Applications: Flow cytometry
    • Target: Vascular endothelial cell growth factor 165 receptor, VEGF165R, CD304
  • Anti-Neuropilin-1 (NRP1) (extracellular) Antibody
    Anti-Neuropilin-1 (NRP1) (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Neuropilin-1
    • Product Code: ANR-063
    • Short Description: A Rabbit Polyclonal Antibody to Neuropilin-1
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunocytochemistry, Immunofluorescence
    • Target: Vascular endothelial cell growth factor 165 receptor, VEGF165R, CD304
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