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  • The expected molecular weight (MW) listed in the product datasheet is based solely on the size of the target protein’s amino acid sequence. It’s important to remember that many factors can affect the banding pattern of your western blot including: (1) the existence of a splice variant, (2) the quality of the loaded sample, (3) the protein extraction method, and (4) the protein transfer conditions. To achieve accurate results, you may need to systematically adjust the protein extraction method or the protein transfer conditions.

    • If the band’s MW is below the expected MW it could be due to a splice variant with a slightly different MW.
      • Heat the samples at 70°C for 10 min.
      • Increase the transfer time.
    • If the band’s MW is above the expected MW, it could be due to post-translational modifications.
    • In either event, you should use a blocking peptide as a negative control.

  • Some toxins and peptides are soluble in DMSO; you can find this information in the product specification section. For these products, prepare a concentrated stock solution first.

    1. Centrifuge vial (10,000 x g, 5 minutes) before adding solvent.
    2. Dissolve the lyophilized reagent in DMSO. We recommend keeping a concentrated stock solution at 1-10 mM (100-1000X higher than the final working concentration).
    3. Once the peptide is completely dissolved in DMSO, slowly dilute the peptide in pure water (or buffer) to the desired final working concentration.

    Note: We recommend that you maintain a DMSO concentration as low as possible. For cell assays, a final concentration of 0.1–0.5% DMSO (v/v) is considered acceptable. For other experiments, 5% DMSO (v/v) is recommended; adjust this according to your experimental requirements.

  • FAQ 3FAQ 3FAQ 3FAQ 3

2041 Results
  • Anti-Synapsin III (SYN3) Antibody
    Anti-Synapsin III (SYN3) Antibody
    A Rabbit Polyclonal Antibody to Synapsin III
    • Product Code: ANR-017
    • Short Description: A Rabbit Polyclonal Antibody to Synapsin III
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: Synapsin-3
  • Guinea Pig Anti-GAP43 Antibody
    Guinea Pig Anti-GAP43 Antibody
    A Guinea Pig Polyclonal Antibody to GAP43
    • Product Code: ANR-016-GP
    • Short Description: A Guinea Pig Polyclonal Antibody to GAP43
    • Host: Guinea Pig
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunohistochemistry
    • Target: Axonal membrane protein GAP-43, Growth-associated protein 43, Neuromodulin, Neural phosphoprotein B-50
  • Anti-GAP43 Antibody
    Anti-GAP43 Antibody
    A Rabbit Polyclonal Antibody to GAP43
    • Product Code: ANR-016
    • Short Description: A Rabbit Polyclonal Antibody to GAP43
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: Axonal membrane protein GAP-43, Growth-associated protein 43, Neuromodulin, Neural phosphoprotein B-50
  • Anti-Synapsin II (SYN2) Antibody
    Anti-Synapsin II (SYN2) Antibody
    A Rabbit Polyclonal Antibody to Synapsin II
    • Product Code: ANR-015
    • Short Description: A Rabbit Polyclonal Antibody to Synapsin II
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: Synapsin-2
  • Anti-Synapsin I (SYN1) Antibody
    Anti-Synapsin I (SYN1) Antibody
    A Rabbit Polyclonal Antibody to Synapsin I
    • Product Code: ANR-014
    • Short Description: A Rabbit Polyclonal Antibody to Synapsin I
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: Synapsin-1
  • Guinea Pig Anti-Synaptophysin Antibody
    Guinea Pig Anti-Synaptophysin Antibody
    A Guinea Pig Polyclonal Antibody to Synaptophysin
    • Product Code: ANR-013-GP
    • Short Description: A Guinea Pig Polyclonal Antibody to Synaptophysin
    • Host: Guinea Pig
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunohistochemistry
    • Target: Major synaptic vesicle protein p38, SYP
  • Anti-Synaptophysin Antibody
    Anti-Synaptophysin Antibody
    A Rabbit Polyclonal Antibody to Synaptophysin
    • Product Code: ANR-013
    • Short Description: A Rabbit Polyclonal Antibody to Synaptophysin
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: Major synaptic vesicle protein p38, SYP
  • Anti-CPLX1/2 Antibody
    Anti-CPLX1/2 Antibody
    A Rabbit Polyclonal Antibody to Complexin-1 and Complexin-2
    • Product Code: ANR-011
    • Short Description: A Rabbit Polyclonal Antibody to Complexin-1 and Complexin-2
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot
    • Target: Complexin 1, Complexin I, Synaphin-2, Complexin 2, Complexin II, Synaphin-1
  • Anti-Syntaxin 2 Antibody
    Anti-Syntaxin 2 Antibody
    A Rabbit Polyclonal Antibody to Syntaxin 2
    • Product Code: ANR-008
    • Short Description: A Rabbit Polyclonal Antibody to Syntaxin 2
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: STX2, Epimorphin, EPIM
  • Anti-VAMP-2 Antibody
    Anti-VAMP-2 Antibody
    A Rabbit Polyclonal Antibody to VAMP-2
    • Product Code: ANR-007
    • Short Description: A Rabbit Polyclonal Antibody to VAMP-2
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: Vesicle-associated membrane protein 2, Synaptobrevin 2, Syb2
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