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  • The expected molecular weight (MW) listed in the product datasheet is based solely on the size of the target protein’s amino acid sequence. It’s important to remember that many factors can affect the banding pattern of your western blot including: (1) the existence of a splice variant, (2) the quality of the loaded sample, (3) the protein extraction method, and (4) the protein transfer conditions. To achieve accurate results, you may need to systematically adjust the protein extraction method or the protein transfer conditions.

    • If the band’s MW is below the expected MW it could be due to a splice variant with a slightly different MW.
      • Heat the samples at 70°C for 10 min.
      • Increase the transfer time.
    • If the band’s MW is above the expected MW, it could be due to post-translational modifications.
    • In either event, you should use a blocking peptide as a negative control.

  • Some toxins and peptides are soluble in DMSO; you can find this information in the product specification section. For these products, prepare a concentrated stock solution first.

    1. Centrifuge vial (10,000 x g, 5 minutes) before adding solvent.
    2. Dissolve the lyophilized reagent in DMSO. We recommend keeping a concentrated stock solution at 1-10 mM (100-1000X higher than the final working concentration).
    3. Once the peptide is completely dissolved in DMSO, slowly dilute the peptide in pure water (or buffer) to the desired final working concentration.

    Note: We recommend that you maintain a DMSO concentration as low as possible. For cell assays, a final concentration of 0.1–0.5% DMSO (v/v) is considered acceptable. For other experiments, 5% DMSO (v/v) is recommended; adjust this according to your experimental requirements.

  • FAQ 3FAQ 3FAQ 3FAQ 3

2041 Results
  • Anti-VMAT2 Antibody
    Anti-VMAT2 Antibody
    A Rabbit Polyclonal Antibody to Vesicular Monoamine Transporter 2
    • Product Code: AMT-006
    • Short Description: A Rabbit Polyclonal Antibody to Vesicular Monoamine Transporter 2
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: Vesicular monoamine transporter 2, SLC18A2, SVAT, VAT2
  • Anti-Serotonin Transporter (SERT) (extracellular)-PE Antibody
    Anti-Serotonin Transporter (SERT) (extracellular)-PE Antibody
    A Rabbit Polyclonal Antibody to Serotonin Transporter (SERT)(extracellular) conjugated to the fluorescent dye Phycoerythrin (PE)
    • Product Code: AMT-004-PE
    • Short Description: A Rabbit Polyclonal Antibody to Serotonin Transporter (SERT)(extracellular) conjugated to the fluorescent dye Phycoerythrin (PE)
    • Host: Rabbit
    • Label: R-PE
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: Sodium-dependent serotonin transporter, 5-HT transporter, 5-HTT, 5-HTTLPR, SLC6A4
  • Anti-Serotonin Transporter (SERT) (extracellular)-FITC Antibody
    Anti-Serotonin Transporter (SERT) (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to Serotonin Transporter (SERT) Conjugated to the Fluorescent Dye FITC
    • Product Code: AMT-004-F
    • Short Description: A Rabbit Polyclonal Antibody to Serotonin Transporter (SERT) Conjugated to the Fluorescent Dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: Sodium-dependent serotonin transporter, 5-HT transporter, 5-HTT, 5-HTTLPR, SLC6A4
  • Anti-Serotonin Transporter (SERT) (extracellular) Antibody
    KO Validate'
    Anti-Serotonin Transporter (SERT) (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Serotonin Transporter (SERT)
    • Product Code: AMT-004
    • Short Description: A Rabbit Polyclonal Antibody to Serotonin Transporter (SERT)
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: Sodium-dependent serotonin transporter, 5-HT transporter, 5-HTT, 5-HTTLPR, SLC6A4
  • Guinea Pig Anti-Dopamine Transporter (DAT) (extracellular) Antibody
    Guinea Pig Anti-Dopamine Transporter (DAT) (extracellular) Antibody
    A Guinea Pig Polyclonal Antibody to Dopamine Transporter (DAT)
    • Product Code: AMT-003-GP
    • Short Description: A Guinea Pig Polyclonal Antibody to Dopamine Transporter (DAT)
    • Host: Guinea Pig
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Western blot, Immunohistochemistry
    • Target: Sodium-dependent dopamine transporter, DA transporter, Solute carrier family 6 member 3, SLC6A3
  • Anti-Dopamine Transporter (DAT) (extracellular)-FITC Antibody
    Anti-Dopamine Transporter (DAT) (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to Dopamine Transporter (DAT) Conjugated to the Fluorescent Dye FITC
    • Product Code: AMT-003-F
    • Short Description: A Rabbit Polyclonal Antibody to Dopamine Transporter (DAT) Conjugated to the Fluorescent Dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: Sodium-dependent dopamine transporter, DA transporter, Solute carrier family 6 member 3, SLC6A3
  • Anti-Dopamine Transporter (DAT) (extracellular) Antibody
    KO Validate'
    Anti-Dopamine Transporter (DAT) (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Dopamine Transporter (DAT)
    • Product Code: AMT-003
    • Short Description: A Rabbit Polyclonal Antibody to Dopamine Transporter (DAT)
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunocytochemistry
    • Target: Sodium-dependent dopamine transporter, DA transporter, SLC6A3
  • Anti-Noradrenaline Transporter (NET) (extracellular) Antibody
    Anti-Noradrenaline Transporter (NET) (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Noradrenaline Transporter (NET)
    • Product Code: AMT-002
    • Short Description: A Rabbit Polyclonal Antibody to Noradrenaline Transporter (NET)
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Live cell imaging, Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: Sodium-dependent noradrenaline transporter, Norepinephrine transporter, SLC6A2
  • Anti-MCHR1 (extracellular) Antibody
    Anti-MCHR1 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Melanin-Concentrating Hormone Receptor 1
    • Product Code: AMR-041
    • Short Description: A Rabbit Polyclonal Antibody to Melanin-Concentrating Hormone Receptor 1
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Western blot, Immunocytochemistry, Immunofluorescence
    • Target: Melanin-concentrating hormone receptor 1, MCH1, MCH1R, GPR24, Somatostatin receptor-like protein, SLC-1
  • Anti-Melatonin Receptor 1B (MTNR1B) Antibody
    Anti-Melatonin Receptor 1B (MTNR1B) Antibody
    A Rabbit Polyclonal Antibody to Melatonin Receptor Type 1B
    • Product Code: AMR-032
    • Short Description: A Rabbit Polyclonal Antibody to Melatonin Receptor Type 1B
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: Melatonin receptor type 2, MT2, Mel1b receptor, Mel-1B-R
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