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  • The expected molecular weight (MW) listed in the product datasheet is based solely on the size of the target protein’s amino acid sequence. It’s important to remember that many factors can affect the banding pattern of your western blot including: (1) the existence of a splice variant, (2) the quality of the loaded sample, (3) the protein extraction method, and (4) the protein transfer conditions. To achieve accurate results, you may need to systematically adjust the protein extraction method or the protein transfer conditions.

    • If the band’s MW is below the expected MW it could be due to a splice variant with a slightly different MW.
      • Heat the samples at 70°C for 10 min.
      • Increase the transfer time.
    • If the band’s MW is above the expected MW, it could be due to post-translational modifications.
    • In either event, you should use a blocking peptide as a negative control.

  • Some toxins and peptides are soluble in DMSO; you can find this information in the product specification section. For these products, prepare a concentrated stock solution first.

    1. Centrifuge vial (10,000 x g, 5 minutes) before adding solvent.
    2. Dissolve the lyophilized reagent in DMSO. We recommend keeping a concentrated stock solution at 1-10 mM (100-1000X higher than the final working concentration).
    3. Once the peptide is completely dissolved in DMSO, slowly dilute the peptide in pure water (or buffer) to the desired final working concentration.

    Note: We recommend that you maintain a DMSO concentration as low as possible. For cell assays, a final concentration of 0.1–0.5% DMSO (v/v) is considered acceptable. For other experiments, 5% DMSO (v/v) is recommended; adjust this according to your experimental requirements.

  • FAQ 3FAQ 3FAQ 3FAQ 3

2041 Results
  • Recombinant mouse proNGF (cleavage resistant) protein
    Bioassay Tested
    Recombinant mouse proNGF (cleavage resistant) protein
    A cleavage-resistant recombinant mouse proNGF that binds TrkA, p75NTR, and SORT1 receptors involved in both cell survival and apoptosis
    • Product Code: N-255
    • Short Description: A cleavage-resistant recombinant mouse proNGF that binds TrkA, p75NTR, and SORT1 receptors involved in both cell survival and apoptosis
    • Applications: Cell Culture, In vivo, Survival assay, Neurite outgrowth assay
    • MW: 50.2 kDa (dimer)
  • Recombinant mouse proNGF protein
    Bioassay Tested
    Recombinant mouse proNGF protein
    A recombinant mouse proNGF that binds TrkA, p75NTR, and SORT1 receptors involved in both cell survival and apoptosis
    • Product Code: N-250
    • Short Description: A recombinant mouse proNGF that binds TrkA, p75NTR, and SORT1 receptors involved in both cell survival and apoptosis
    • Applications: Cell Culture, proNGF digestion assay, Survival assay, Neurite outgrowth assay
    • MW: 50.4 kDa (dimer)
  • Recombinant human beta-NGF protein
    Bioassay Tested
    Recombinant human beta-NGF protein
    A recombinant human neurotrophin that promotes neuronal survival and neurite outgrowth via TrkA and p75NTR receptors
    • Product Code: N-245
    • Short Description: A recombinant human neurotrophin that promotes neuronal survival and neurite outgrowth via TrkA and p75NTR receptors
    • Applications: Cell Culture, Survival assay, Neurite outgrowth assay
    • MW: 27 kDa (dimer)
  • mouse NGF 2.5S-Biotin
    Bioassay Tested
    mouse NGF 2.5S-Biotin
    A biotinylated mouse NGF 2.5S for imaging and sensitive detection of TrkA and p75NTR receptors
    • Product Code: N-240-B
    • Short Description: A biotinylated mouse NGF 2.5S for imaging and sensitive detection of TrkA and p75NTR receptors
    • Label: Biotin
    • Applications: Cell Culture, Survival assay, Fluorescence staining, Live cell imaging, Immunofluorescence, Neurite outgrowth assay
    • MW: ~26 kDa
  • Native mouse NGF 2.5S protein (99%)
    Bioassay Tested
    Native mouse NGF 2.5S protein (99%)
    A native mouse neurotrophin purified from submaxillary glands that promotes neuronal survival and neurite outgrowth via TrkA and p75NTR receptors
    • Product Code: N-240
    • Short Description: A native mouse neurotrophin purified from submaxillary glands that promotes neuronal survival and neurite outgrowth via TrkA and p75NTR receptors
    • Applications: Cell Culture, Survival assay, Neurite outgrowth assay
    • CAS No.: 93928-24-6
    • MW: 26 kDa
  • Native mouse NGF 7S protein
    Bioassay Tested
    Native mouse NGF 7S protein
    A native mouse nerve growth factor (NGF) 7S protein complex that promotes neuronal survival and neurite outgrowth via TrkA and p75NTR receptors
    • Product Code: N-130
    • Short Description: A native mouse nerve growth factor (NGF) 7S protein complex that promotes neuronal survival and neurite outgrowth via TrkA and p75NTR receptors
    • Applications: Cell Culture, Survival assay, Neurite outgrowth assay
    • CAS No.: 93928-24-6
    • MW: 130 kDa
  • Native mouse NGF 2.5S protein (>95%)
    Bioassay Tested
    Native mouse NGF 2.5S protein (>95%)
    A native mouse neurotrophin purified from submaxillary glands that promotes neuronal survival and neurite outgrowth via TrkA and p75NTR receptors
    • Product Code: N-100
    • Short Description: A native mouse neurotrophin purified from submaxillary glands that promotes neuronal survival and neurite outgrowth via TrkA and p75NTR receptors
    • Applications: Cell Culture, ELISA control, Survival assay, Neurite outgrowth assay
    • CAS No.: 93928-24-6
    • MW: 26 kDa
  • Recombinant human LIF protein
    Bioassay Tested
    Recombinant human LIF protein
    A recombinant human Leukemia Inhibitory Factor (LIF) cytokine that promotes stem cell self-renewal and inhibits differentiation by signaling through the LIFR/gp130 receptor complex
    • Product Code: L-200
    • Short Description: A recombinant human Leukemia Inhibitory Factor (LIF) cytokine that promotes stem cell self-renewal and inhibits differentiation by signaling through the LIFR/gp130 receptor complex
    • Applications: Cell Culture, Proliferation assay, Western blot
    • MW: 19.7 kDa
  • Recombinant human GDNF protein
    Bioassay Tested
    Recombinant human GDNF protein
    A recombinant human glial cell line-derived neurotrophic factor (GDNF) that enhances the survival and differentiation of dopaminergic and motor neurons via GFRα1/RET receptor complex
    • Product Code: G-240
    • Short Description: A recombinant human glial cell line-derived neurotrophic factor (GDNF) that enhances the survival and differentiation of dopaminergic and motor neurons via GFRα1/RET receptor complex
    • Applications: Axon growth, Cell Culture, Western blot
    • MW: 30.1 kDa (dimer)
  • Recombinant human FGF-b protein
    Bioassay Tested
    Recombinant human FGF-b protein
    A recombinant human growth factor (FGF-2/bFGF) that promotes cell proliferation, angiogenesis, and neuronal survival via FGFR receptors
    • Product Code: F-170
    • Short Description: A recombinant human growth factor (FGF-2/bFGF) that promotes cell proliferation, angiogenesis, and neuronal survival via FGFR receptors
    • Applications: Cell Culture, Neurosphere formation, Proliferation assay
    • MW: 16.5 kDa
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