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  • The expected molecular weight (MW) listed in the product datasheet is based solely on the size of the target protein’s amino acid sequence. It’s important to remember that many factors can affect the banding pattern of your western blot including: (1) the existence of a splice variant, (2) the quality of the loaded sample, (3) the protein extraction method, and (4) the protein transfer conditions. To achieve accurate results, you may need to systematically adjust the protein extraction method or the protein transfer conditions.

    • If the band’s MW is below the expected MW it could be due to a splice variant with a slightly different MW.
      • Heat the samples at 70°C for 10 min.
      • Increase the transfer time.
    • If the band’s MW is above the expected MW, it could be due to post-translational modifications.
    • In either event, you should use a blocking peptide as a negative control.

  • Some toxins and peptides are soluble in DMSO; you can find this information in the product specification section. For these products, prepare a concentrated stock solution first.

    1. Centrifuge vial (10,000 x g, 5 minutes) before adding solvent.
    2. Dissolve the lyophilized reagent in DMSO. We recommend keeping a concentrated stock solution at 1-10 mM (100-1000X higher than the final working concentration).
    3. Once the peptide is completely dissolved in DMSO, slowly dilute the peptide in pure water (or buffer) to the desired final working concentration.

    Note: We recommend that you maintain a DMSO concentration as low as possible. For cell assays, a final concentration of 0.1–0.5% DMSO (v/v) is considered acceptable. For other experiments, 5% DMSO (v/v) is recommended; adjust this according to your experimental requirements.

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2044 Results
  • Recombinant human proBDNF protein
    Bioassay Tested
    Recombinant human proBDNF protein
    A recombinant human proBDNF protein produced in E. coli that functions exclusively as a ligand for the p75NTR/sortilin receptor complex
    • Product Code: B-257
    • Short Description: A recombinant human proBDNF protein produced in E. coli that functions exclusively as a ligand for the p75NTR/sortilin receptor complex
    • Applications: Cell Culture, In vitro binding assay, Western blot
    • MW: 51.78 kDa (dimer)
  • Recombinant human proBDNF (cleavage resistant) protein
    Bioassay Tested
    Recombinant human proBDNF (cleavage resistant) protein
    A recombinant human, cleavage-resistant proBDNF protein produced in E. coli that functions exclusively as a ligand for the p75NTR/sortilin receptor complex
    • Product Code: B-256
    • Short Description: A recombinant human, cleavage-resistant proBDNF protein produced in E. coli that functions exclusively as a ligand for the p75NTR/sortilin receptor complex
    • Applications: Cell Culture, Western blot
    • MW: 51.41 kDa (dimer)
  • human BDNF-Biotin
    Bioassay Tested
    human BDNF-Biotin
    A biotinylated human BDNF for sensitive detection of TrkB, p75NTR receptors, and the study of neuronal survival and synaptic plasticity
    • Product Code: B-250-B
    • Short Description: A biotinylated human BDNF for sensitive detection of TrkB, p75NTR receptors, and the study of neuronal survival and synaptic plasticity
    • Label: Biotin
    • Applications: Apoptosis assay, Cell Culture, Differentiation assay, Ex vivo, In vivo, Survival assay, Fluorescence staining, Live cell imaging, Immunofluorescence, Western blot
    • MW: ~28 kDa (dimer)
  • Recombinant human BDNF protein
    Bioassay Tested
    Recombinant human BDNF protein
    A recombinant human mature BDNF protein produced in E. coli that functions as a ligand for the TrkB receptors
    • Product Code: B-250
    • Short Description: A recombinant human mature BDNF protein produced in E. coli that functions as a ligand for the TrkB receptors
    • Applications: Apoptosis assay, Cell Culture, Differentiation assay, Ex vivo, In vivo, Survival assay, Western blot
    • MW: 27 kDa (dimer)
  • Recombinant human BDNF proDomain protein
    Bioassay Tested
    Recombinant human BDNF proDomain protein
    A recombinant human BDNF prodomain protein produced in E. coli that functions as a ligand for the sortilin receptor and potentially GIRK channels
    • Product Code: B-245
    • Short Description: A recombinant human BDNF prodomain protein produced in E. coli that functions as a ligand for the sortilin receptor and potentially GIRK channels
    • Applications: Plasmin digestion assay, Western blot
    • MW: 12.4 kDa
  • Recombinant mouse proBDNF (cleavage resistant) protein
    Bioassay Tested
    Recombinant mouse proBDNF (cleavage resistant) protein
    A recombinant mouse, cleavage-resistant proBDNF protein produced in E. coli that functions as a ligand for the p75NTR/sortilin receptor complex
    • Product Code: B-243
    • Short Description: A recombinant mouse, cleavage-resistant proBDNF protein produced in E. coli that functions as a ligand for the p75NTR/sortilin receptor complex
    • Applications: Cell Culture, In vivo, Electrophysiology, Western blot
    • MW: 52 kDa (dimer)
  • Recombinant mouse proBDNF protein
    Bioassay Tested
    Recombinant mouse proBDNF protein
    A recombinant mouse proBDNF protein produced in E. coli that functions as a ligand for the p75NTR/sortilin receptor complex
    • Product Code: B-240
    • Short Description: A recombinant mouse proBDNF protein produced in E. coli that functions as a ligand for the p75NTR/sortilin receptor complex
    • Applications: In vitro, In vivo, Electrophysiology, Western blot
    • MW: 52.4 kDa (dimer)
  • α-Bungarotoxin
    Bioassay Tested
    α-Bungarotoxin
    A potent antagonist of muscle nAChRs, α7 nAChRs, and GABA(A) receptor subtypes
    • Product Code: B-100
    • Short Description: A potent antagonist of muscle nAChRs, α7 nAChRs, and GABA(A) receptor subtypes
    • Applications: Electrophysiology
    • CAS No.: 11032-79-4
    • MW: 7984 Da
  • Anti-Na<sub>V</sub>1.7 (SCN9A) (extracellular) Antibody
    Anti-NaV1.7 (SCN9A) (extracellular) Antibody
    A Rabbit Polyclonal Antibody to NaV1.7 (SCN9A) Channel
    • Product Code: ASC-027
    • Short Description: A Rabbit Polyclonal Antibody to NaV1.7 (SCN9A) Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat
    • Applications: Live cell imaging, Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: Voltage-gated sodium channel type IX subunit alpha, PN1, NENA, NE, ETHA
  • Anti-Na<sub>V</sub>1.7 (SCN9A) Antibody
    KO Validate'
    Anti-NaV1.7 (SCN9A) Antibody
    A Rabbit Polyclonal Antibody to NaV1.7 (SCN9A) Channel
    • Product Code: ASC-008
    • Short Description: A Rabbit Polyclonal Antibody to NaV1.7 (SCN9A) Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: Voltage-gated sodium channel type IX subunit alpha, PN1, NENA, NE, ETHA
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