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  • The expected molecular weight (MW) listed in the product datasheet is based solely on the size of the target protein’s amino acid sequence. It’s important to remember that many factors can affect the banding pattern of your western blot including: (1) the existence of a splice variant, (2) the quality of the loaded sample, (3) the protein extraction method, and (4) the protein transfer conditions. To achieve accurate results, you may need to systematically adjust the protein extraction method or the protein transfer conditions.

    • If the band’s MW is below the expected MW it could be due to a splice variant with a slightly different MW.
      • Heat the samples at 70°C for 10 min.
      • Increase the transfer time.
    • If the band’s MW is above the expected MW, it could be due to post-translational modifications.
    • In either event, you should use a blocking peptide as a negative control.

  • Some toxins and peptides are soluble in DMSO; you can find this information in the product specification section. For these products, prepare a concentrated stock solution first.

    1. Centrifuge vial (10,000 x g, 5 minutes) before adding solvent.
    2. Dissolve the lyophilized reagent in DMSO. We recommend keeping a concentrated stock solution at 1-10 mM (100-1000X higher than the final working concentration).
    3. Once the peptide is completely dissolved in DMSO, slowly dilute the peptide in pure water (or buffer) to the desired final working concentration.

    Note: We recommend that you maintain a DMSO concentration as low as possible. For cell assays, a final concentration of 0.1–0.5% DMSO (v/v) is considered acceptable. For other experiments, 5% DMSO (v/v) is recommended; adjust this according to your experimental requirements.

  • FAQ 3FAQ 3FAQ 3FAQ 3

2044 Results
  • Anti-Nicotinic Acetylcholine Receptor α3 (CHRNA3) (extracellular) Antibody
    Anti-Nicotinic Acetylcholine Receptor α3 (CHRNA3) (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Nicotinic Acetylcholine Receptor α3
    • Product Code: ANC-003
    • Short Description: A Rabbit Polyclonal Antibody to Nicotinic Acetylcholine Receptor α3
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: nAChR α3, Neuronal acetylcholine receptor subunit alpha-3, ACHA3, PAOD2
  • Anti-GABA(A) α1 Receptor (extracellular)-FITC Antibody
    Anti-GABA(A) α1 Receptor (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to GABRA1 (extracellular) conjugated to the fluorescent dye FITC
    • Product Code: AGA-001-F
    • Short Description: A Rabbit Polyclonal Antibody to GABRA1 (extracellular) conjugated to the fluorescent dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: γ-Aminobutyric acid receptor type A subunit α1, GABRA1
  • α-Dendrotoxin-ATTO Fluor-647N
    Bioassay Tested
    α-Dendrotoxin-ATTO Fluor-647N
    A fluorescent probe for imaging and sensitive detection of Kv1.1, Kv1.2, and Kv1.6 channels.
    • Product Code: D-350-FRN
    • Short Description: A fluorescent probe for imaging and sensitive detection of Kv1.1, Kv1.2, and Kv1.6 channels.
    • Label: ATTO 647N
    • Applications: Electrophysiology, Direct flow cytometry, Fluorescence staining, Live cell imaging, Immunofluorescence
    • MW: ~7990 Da
  • OXGR1/GPR99 (extracellular) Blocking Peptide
    OXGR1/GPR99 (extracellular) Blocking Peptide
    A Blocking Peptide for Ant-OXGR1/GPR99 (extracellular) Antibody
    • Product Code: BLP-OR017
    • Short Description: A Blocking Peptide for Ant-OXGR1/GPR99 (extracellular) Antibody
    • Applications: Western blot, Immunohistochemistry
    • Target: GPR99
  • CHRM3 (extracellular) Blocking Peptide
    CHRM3 (extracellular) Blocking Peptide
    A Blocking Peptide for Anti-CHRM3 (extracellular) Antibody
    • Product Code: BLP-MR013
    • Short Description: A Blocking Peptide for Anti-CHRM3 (extracellular) Antibody
    • Applications: Western blot, Immunohistochemistry
    • Target: CHRM3
  • α-Bungarotoxin-ATTO Fluor-647N
    Bioassay Tested
    α-Bungarotoxin-ATTO Fluor-647N
    A fluorescent probe for imaging and sensitive detection of neuromuscular junctions, GABA(A) receptors, nAChRs, and α-Bungarotoxin binding sites
    • Product Code: B-100-FRN
    • Short Description: A fluorescent probe for imaging and sensitive detection of neuromuscular junctions, GABA(A) receptors, nAChRs, and α-Bungarotoxin binding sites
    • Label: ATTO 647N
    • Applications: Electrophysiology, Direct flow cytometry, Fluorescence staining, Live cell imaging, Immunofluorescence
    • MW: ~8925 Da
  • Anti-OXGR1/GPR99 (extracellular) Antibody
    Anti-OXGR1/GPR99 (extracellular) Antibody
    A rabbit polyclonal antibody to OXGR1/GPR99 (extracellular)
    • Product Code: AOR-017
    • Short Description: A rabbit polyclonal antibody to OXGR1/GPR99 (extracellular)
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Western blot, Immunohistochemistry
    • Target: Oxoglutarate receptor 1, 2-Oxoglutarate receptor 1, Alpha-ketoglutarate receptor 1, AKGR, GPR80, P2RY15, P2Y15, CysLTR3
  • Anti-C3aR1 (extracellular)-mFluor™ Violet 450 Antibody
    Anti-C3aR1 (extracellular)-mFluor™ Violet 450 Antibody
    A Rabbit Polyclonal Antibody to C3aR1 (extracellular) conjugated to the fluorescent dye mFluor™ Violet 450 fluorophore.
    • Product Code: AAR-036-V
    • Short Description: A Rabbit Polyclonal Antibody to C3aR1 (extracellular) conjugated to the fluorescent dye mFluor™ Violet 450 fluorophore.
    • Host: Rabbit
    • Label: mFluor™ Violet 450
    • Reactivity: Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: Complement C3a Receptor 1, C3AR, C3a Anaphylatoxin Chemotactic Receptor
  • Tityustoxin-Kα-ATTO Fluor-594
    Bioassay Tested
    Tityustoxin-Kα-ATTO Fluor-594
    A fluorescent probe for imaging and sensitive detection of KV1.2, KV1.3, KV1.1, and KV1.6 potassium channels
    • Product Code: STT-360-AR
    • Short Description: A fluorescent probe for imaging and sensitive detection of KV1.2, KV1.3, KV1.1, and KV1.6 potassium channels
    • Label: ATTO 594
    • Applications: Electrophysiology, Direct flow cytometry, Live cell imaging, Immunofluorescence
    • MW: ~4900 Da
  • Tertiapin-Q-ATTO Fluor-633
    Bioassay Tested
    Tertiapin-Q-ATTO Fluor-633
    A fluorescent probe for imaging and sensitive detection of Kir1.1 and GIRK potassium channels
    • Product Code: STT-170-FR
    • Short Description: A fluorescent probe for imaging and sensitive detection of Kir1.1 and GIRK potassium channels
    • Label: ATTO 633
    • Applications: Electrophysiology, Fluorescence staining, Live cell imaging, Immunofluorescence
    • MW: 3159.6 Da
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