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  • The expected molecular weight (MW) listed in the product datasheet is based solely on the size of the target protein’s amino acid sequence. It’s important to remember that many factors can affect the banding pattern of your western blot including: (1) the existence of a splice variant, (2) the quality of the loaded sample, (3) the protein extraction method, and (4) the protein transfer conditions. To achieve accurate results, you may need to systematically adjust the protein extraction method or the protein transfer conditions.

    • If the band’s MW is below the expected MW it could be due to a splice variant with a slightly different MW.
      • Heat the samples at 70°C for 10 min.
      • Increase the transfer time.
    • If the band’s MW is above the expected MW, it could be due to post-translational modifications.
    • In either event, you should use a blocking peptide as a negative control.

  • Some toxins and peptides are soluble in DMSO; you can find this information in the product specification section. For these products, prepare a concentrated stock solution first.

    1. Centrifuge vial (10,000 x g, 5 minutes) before adding solvent.
    2. Dissolve the lyophilized reagent in DMSO. We recommend keeping a concentrated stock solution at 1-10 mM (100-1000X higher than the final working concentration).
    3. Once the peptide is completely dissolved in DMSO, slowly dilute the peptide in pure water (or buffer) to the desired final working concentration.

    Note: We recommend that you maintain a DMSO concentration as low as possible. For cell assays, a final concentration of 0.1–0.5% DMSO (v/v) is considered acceptable. For other experiments, 5% DMSO (v/v) is recommended; adjust this according to your experimental requirements.

  • FAQ 3FAQ 3FAQ 3FAQ 3

2041 Results
  • human proBDNF-Biotin
    Bioassay Tested
    human proBDNF-Biotin
    A biotinylated human proBDNF for sensitive detection of p75NTR, sortilin receptors, and the study of neuronal apoptosis and long-term depression (LTD)
    • Product Code: B-257-B
    • Short Description: A biotinylated human proBDNF for sensitive detection of p75NTR, sortilin receptors, and the study of neuronal apoptosis and long-term depression (LTD)
    • Label: Biotin
    • Applications: Cell Culture, In vitro binding assay, Western blot, Live imaging
    • MW: ~52.5 kDa (dimer)
  • human proBDNF-Biotin
    Bioassay Tested
    human proBDNF-Biotin
    A biotinylated human proBDNF for sensitive detection of p75NTR, sortilin receptors, and the study of neuronal apoptosis and long-term depression (LTD)
    • Product Code: B-256-B
    • Short Description: A biotinylated human proBDNF for sensitive detection of p75NTR, sortilin receptors, and the study of neuronal apoptosis and long-term depression (LTD)
    • Label: Biotin
    • Applications: Cell Culture, Western blot, Live imaging
    • MW: ~52 kDa (dimer)
  • α-Bungarotoxin-FITC
    Bioassay Tested
    α-Bungarotoxin-FITC
    A fluorescent probe for imaging and sensitive detection of neuromuscular junctions, GABA(A) receptors, nAChRs, and α-Bungarotoxin binding sites
    • Product Code: B-100-F
    • Short Description: A fluorescent probe for imaging and sensitive detection of neuromuscular junctions, GABA(A) receptors, nAChRs, and α-Bungarotoxin binding sites
    • Label: FITC
    • Applications: Electrophysiology, Fluorescence staining
    • MW: ~8406 Da
  • α-Bungarotoxin-Biotin
    Bioassay Tested
    α-Bungarotoxin-Biotin
    A biotinylated probe for imaging and sensitive detection of neuromuscular junctions, GABA(A) receptors, nAChRs, and α-Bungarotoxin binding sites
    • Product Code: B-100-B
    • Short Description: A biotinylated probe for imaging and sensitive detection of neuromuscular junctions, GABA(A) receptors, nAChRs, and α-Bungarotoxin binding sites
    • Label: Biotin
    • Applications: Electrophysiology, Immunofluorescence, Indirect flow cytometry
    • MW: ~9030 Da
  • ZINC9315614
    Bioassay Tested
    ZINC9315614
    An Antagonist of P2X7 Receptors
    • Product Code: Z-145
    • Short Description: An Antagonist of P2X7 Receptors
    • CAS No.: NA
    • MW: 328.74
  • ZINC58368839
    Bioassay Tested
    ZINC58368839
    An Antagonist of P2X7 Receptors
    • Product Code: Z-135
    • Short Description: An Antagonist of P2X7 Receptors
    • CAS No.: NA
    • MW: 329.39
  • Zibotentan
    Bioassay Tested
    Zibotentan
    A Selective Antagonist of Endothelin A Receptors
    • Product Code: Z-130
    • Short Description: A Selective Antagonist of Endothelin A Receptors
    • CAS No.: 186497-07-4
    • MW: 424.43
  • Zacopride (hydrochloride)
    Bioassay Tested
    Zacopride (hydrochloride)
    A Potent Antagonist of 5-HT3 Receptors and an Agonist of 5-HT4 Receptors
    • Product Code: Z-110
    • Short Description: A Potent Antagonist of 5-HT3 Receptors and an Agonist of 5-HT4 Receptors
    • CAS No.: 101303-98-4
    • MW: 346.25
  • XEN907
    Bioassay Tested
    XEN907
    A Specific Blocker of NaV1.7 Channels
    • Product Code: X-105
    • Short Description: A Specific Blocker of NaV1.7 Channels
    • CAS No.: 912656-34-9
    • MW: 351.1
  • Wortmannin
    Bioassay Tested
    Wortmannin
    A Selective, Potent Inhibitor of PI3-Kinase
    • Product Code: W-400
    • Short Description: A Selective, Potent Inhibitor of PI3-Kinase
    • CAS No.: 19545-26-7
    • MW: 428.4
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